Immunology Antibodies 2
Anti-SKP2 Antibody (CAB7728)
- SKU:
- CAB7728
- Product Type:
- Antibody
- Reactivity:
- Human
- Reactivity:
- Mouse
- Reactivity:
- Rat
- Host Species:
- Rabbit
- Isotype:
- IgG
- Antibody Type:
- Polyclonal Antibody
- Research Area:
- Immunology
Description
| Antibody Name: | Anti-SKP2 Antibody |
| Antibody SKU: | CAB7728 |
| Antibody Size: | 20uL, 50uL, 100uL |
| Application: | WB IF |
| Reactivity: | Human, Mouse, Rat |
| Host Species: | Rabbit |
| Immunogen: | A synthetic peptide corresponding to a sequence within amino acids 1-100 of human SKP2 (NP_005974.2). |
| Application: | WB IF |
| Recommended Dilution: | WB 1:500 - 1:2000 IF 1:50 - 1:200 |
| Reactivity: | Human, Mouse, Rat |
| Positive Samples: | LO2, HeLa, DU145, Mouse thymus, Mouse spleen, Rat thymus, Rat spleen |
| Immunogen: | A synthetic peptide corresponding to a sequence within amino acids 1-100 of human SKP2 (NP_005974.2). |
| Purification Method: | Affinity purification |
| Storage Buffer: | Store at -20'C. Avoid freeze / thaw cycles. Buffer: PBS with 0.02% sodium azide, 50% glycerol, pH7.3. |
| Isotype: | IgG |
| Sequence: | MHRK HLQE IPDL SSNV ATSF TWGW DSSK TSEL LSGM GVSA LEKE EPDS ENIP QELL SNLG HPES PPRK RLKS KGSD KDFV IVRR PKLN RENF PGVS WDSL |
| Gene ID: | 6502 |
| Uniprot: | Q13309 |
| Cellular Location: | Cytoplasm, Nucleus |
| Calculated MW: | 23kDa/46kDa/47kDa |
| Observed MW: | 45kDa |
| Synonyms: | SKP2, FBL1, FBXL1, FLB1, p45 |
| Background: | This gene encodes a member of the F-box protein family which is characterized by an approximately 40 amino acid motif, the F-box. The F-box proteins constitute one of the four subunits of ubiquitin protein ligase complex called SCFs (SKP1-cullin-F-box), which function in phosphorylation-dependent ubiquitination. The F-box proteins are divided into 3 classes: Fbws containing WD-40 domains, Fbls containing leucine-rich repeats, and Fbxs containing either different protein-protein interaction modules or no recognizable motifs. The protein encoded by this gene belongs to the Fbls class; in addition to an F-box, this protein contains 10 tandem leucine-rich repeats. This protein is an essential element of the cyclin A-CDK2 S-phase kinase. It specifically recognizes phosphorylated cyclin-dependent kinase inhibitor 1B (CDKN1B, also referred to as p27 or KIP1) predominantly in S phase and interacts with S-phase kinase-associated protein 1 (SKP1 or p19). In addition, this gene is established as a protooncogene causally involved in the pathogenesis of lymphomas. Alternative splicing of this gene generates three transcript variants encoding different isoforms. |
| UniProt Protein Function: | SKP2: an F-box protein of the SCF (SKP1-CUL1-F- box protein) E3 ubiquitin ligase system. The F-box component of the SCF complex is responsible for recognizing different substrates for ubiquitination. The SCF E3 complex mediates the ubiquitination and subsequent proteasomal degradation of target proteins involved in cell cycle progression, signal transduction and transcription. Specifically recognizes phosphorylated CDKN1B/p27kip and is involved in regulation of G1/S transition. Degradation of CDKN1B/p27kip also requires CKS1. Three alternatively-spliced isoforms have been described. |
| UniProt Protein Details: | Protein type:Ubiquitin conjugating system; Cell cycle regulation Chromosomal Location of Human Ortholog: 5p13 Cellular Component: nucleoplasm; cytoplasm; nucleolus; SCF ubiquitin ligase complex; nucleus; cytosol Molecular Function:identical protein binding; protein binding; ubiquitin-protein ligase activity Biological Process: regulation of apoptosis; cell proliferation; protein polyubiquitination; regulation of cell cycle; anaphase-promoting complex-dependent proteasomal ubiquitin-dependent protein catabolic process; positive regulation of smooth muscle cell proliferation; positive regulation of estrogen receptor signaling pathway; mitotic cell cycle; G2/M transition of mitotic cell cycle; G1/S transition of mitotic cell cycle |
| NCBI Summary: | This gene encodes a member of the F-box protein family which is characterized by an approximately 40 amino acid motif, the F-box. The F-box proteins constitute one of the four subunits of ubiquitin protein ligase complex called SCFs (SKP1-cullin-F-box), which function in phosphorylation-dependent ubiquitination. The F-box proteins are divided into 3 classes: Fbws containing WD-40 domains, Fbls containing leucine-rich repeats, and Fbxs containing either different protein-protein interaction modules or no recognizable motifs. The protein encoded by this gene belongs to the Fbls class; in addition to an F-box, this protein contains 10 tandem leucine-rich repeats. This protein is an essential element of the cyclin A-CDK2 S-phase kinase. It specifically recognizes phosphorylated cyclin-dependent kinase inhibitor 1B (CDKN1B, also referred to as p27 or KIP1) predominantly in S phase and interacts with S-phase kinase-associated protein 1 (SKP1 or p19). In addition, this gene is established as a protooncogene causally involved in the pathogenesis of lymphomas. Alternative splicing of this gene generates three transcript variants encoding different isoforms. [provided by RefSeq, Jul 2011] |
| UniProt Code: | Q13309 |
| NCBI GenInfo Identifier: | 37537922 |
| NCBI Gene ID: | 6502 |
| NCBI Accession: | Q13309.2 |
| UniProt Secondary Accession: | Q13309,Q8TDZ0, Q8TDZ1, Q9BV69, A8K5E0, B4DJT4, |
| UniProt Related Accession: | Q13309 |
| Molecular Weight: | 23,763 Da |
| NCBI Full Name: | S-phase kinase-associated protein 2 |
| NCBI Synonym Full Names: | S-phase kinase-associated protein 2, E3 ubiquitin protein ligase |
| NCBI Official Symbol: | SKP2 |
| NCBI Official Synonym Symbols: | p45; FBL1; FLB1; FBXL1 |
| NCBI Protein Information: | S-phase kinase-associated protein 2; p45skp2; F-box/LRR-repeat protein 1; CDK2/cyclin A-associated protein p45; S-phase kinase-associated protein 2 (p45) |
| UniProt Protein Name: | S-phase kinase-associated protein 2 |
| UniProt Synonym Protein Names: | Cyclin-A/CDK2-associated protein p45; F-box protein Skp2; F-box/LRR-repeat protein 1; p45skp2 |
| Protein Family: | S-phase kinase-associated protein |
| UniProt Gene Name: | SKP2 |
| UniProt Entry Name: | SKP2_HUMAN |
 | Western blot analysis of extracts of various cell lines, using SKP2 antibody (CAB7728) at 1:1000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (CABS014) at 1:10000 dilution. Lysates/proteins: 25ug per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit (RM00020). Exposure time: 30s. |
 | Immunofluorescence analysis of U2OS cells using SKP2 Rabbit pAb (CAB7728) at dilution of 1:100. Blue: DAPI for nuclear staining. |
 | Immunofluorescence analysis of L929 cells using SKP2 Rabbit pAb (CAB7728) at dilution of 1:100. Blue: DAPI for nuclear staining. |
 | Immunofluorescence analysis of C6 cells using SKP2 Rabbit pAb (CAB7728) at dilution of 1:100. Blue: DAPI for nuclear staining. |
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