Antibody Name:	Anti-SKIL Antibody
Antibody SKU:	CAB5844
Antibody Size:	20uL, 50uL, 100uL
Application:	WB IHC
Reactivity:	Human, Mouse, Rat
Host Species:	Rabbit
Immunogen:	Recombinant fusion protein containing a sequence corresponding to amino acids 420-684 of human SKIL (NP_001234937.1).

Application:	WB IHC
Recommended Dilution:	WB 1:500 - 1:2000 IHC 1:50 - 1:200
Reactivity:	Human, Mouse, Rat
Positive Samples:	Mouse skeletal muscle, Mouse heart, Rat skeletal muscle, Rat heart

Immunogen:	Recombinant fusion protein containing a sequence corresponding to amino acids 420-684 of human SKIL (NP_001234937.1).
Purification Method:	Affinity purification
Storage Buffer:	Store at -20'C. Avoid freeze / thaw cycles. Buffer: PBS with 0.02% sodium azide, 50% glycerol, pH7.3.
Isotype:	IgG
Sequence:	SQSK ELTK TEAS KSIS RQSE KAHS SGKL QKTV SYPD VSLE EQEK MDLK TSRE LCSR LDAS ISNN STSK RKSE SATC NLVR DINK VGIG LVAA ASSP LLVK DVIC EDDK GKIM EEVM RTYL KQQE KLNL ILQK KQQL QMEV KMLS SSKS MKEL TEEQ QNLQ KELE SLQN EHAQ RMEE FYVE QKDL EKKL EQIM KQKC TCDS NLEK DKEA EYAG QLAE LRQR LDHA EADR QELQ DELR QERE ARQK LEMM IKEL KLQI LKSS KTAK E
Gene ID:	6498
Uniprot:	P12757
Cellular Location:
Calculated MW:	44kDa/46kDa/71kDa/74kDa/76kDa
Observed MW:	77kDa

Synonyms:

SKIL, SNO, SnoA, SnoI, SnoN

Background:

The protein encoded by this gene is a component of the SMAD pathway, which regulates cell growth and differentiation through transforming growth factor-beta (TGFB). In the absence of ligand, the encoded protein binds to the promoter region of TGFB-responsive genes and recruits a nuclear repressor complex. TGFB signaling causes SMAD3 to enter the nucleus and degrade this protein, allowing these genes to be activated. Four transcript variants encoding three different isoforms have been found for this gene.

UniProt Protein Function:	SKIL: May have regulatory role in cell division or differentiation in response to extracellular signals. Interacts with SMAD2, SMAD3 and RNF111. Isoform 1 interacts with WWP1. Isoform SNON and isoform SNOA are widely expressed. Highest expression is found in skeletal muscle, followed by placenta and lung. Lowest expression in heart, brain and pancreas. Isoform SNOI expression is restricted to skeletal muscle. Belongs to the SKI family. 5 isoforms of the human protein are produced by alternative splicing.
UniProt Protein Details:	Chromosomal Location of Human Ortholog: 3q26 Cellular Component: acrosome; cytoplasm; nucleoplasm; nucleus; PML body; protein complex Molecular Function:chromatin binding; DNA binding; protein binding; protein complex binding; protein domain specific binding; SMAD binding; transcription corepressor activity Biological Process: blastocyst formation; cell cycle arrest; gene expression; lymphocyte homeostasis; negative regulation of cell differentiation; negative regulation of transcription from RNA polymerase II promoter; negative regulation of transforming growth factor beta receptor signaling pathway; positive regulation of axonogenesis; regulation of apoptosis; response to antibiotic; response to cytokine stimulus; skeletal muscle development; spermatogenesis; transcription initiation from RNA polymerase II promoter; transcription, DNA-dependent; transforming growth factor beta receptor signaling pathway
NCBI Summary:	The protein encoded by this gene is a component of the SMAD pathway, which regulates cell growth and differentiation through transforming growth factor-beta (TGFB). In the absence of ligand, the encoded protein binds to the promoter region of TGFB-responsive genes and recruits a nuclear repressor complex. TGFB signaling causes SMAD3 to enter the nucleus and degrade this protein, allowing these genes to be activated. Four transcript variants encoding three different isoforms have been found for this gene. [provided by RefSeq, Oct 2011]
UniProt Code:	P12757
NCBI GenInfo Identifier:	313104010
NCBI Gene ID:	6498
NCBI Accession:	P12757.2
UniProt Secondary Accession:	P12757,O00464, P12756, Q07501, A6NGT1, B4DT50,
UniProt Related Accession:	P12757
Molecular Weight:	74,784 Da
NCBI Full Name:	Ski-like protein
NCBI Synonym Full Names:	SKI-like proto-oncogene
NCBI Official Symbol:	SKIL
NCBI Official Synonym Symbols:	SNO; SnoA; SnoI; SnoN
NCBI Protein Information:	ski-like protein
UniProt Protein Name:	Ski-like protein
UniProt Synonym Protein Names:	Ski-related oncogene; Ski-related protein
Protein Family:	Ski-like protein
UniProt Gene Name:	SKIL
UniProt Entry Name:	SKIL_HUMAN

	Western blot analysis of extracts of various cell lines, using SKIL antibody (CAB5844) at 1:3000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (CABS014) at 1:10000 dilution. Lysates/proteins: 25ug per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit (RM00020). Exposure time: 3s.
	Immunohistochemistry of paraffin-embedded human mammary cancer using SKIL antibody (CAB5844) at dilution of 1:100 (40x lens).