Immunology Antibodies 2

Anti-SKAP1 Antibody (CAB3345)

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SKU:
CAB3345
Product Type:
Antibody
Reactivity:
Mouse
Reactivity:
Rat
Host Species:
Rabbit
Isotype:
IgG
Research Area:
Immunology

Description

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Antibody Name:Anti-SKAP1 Antibody
Antibody SKU:CAB3345
Antibody Size:20uL, 50uL, 100uL
Application:WB
Reactivity:Mouse, Rat
Host Species:Rabbit
Immunogen:Recombinant fusion protein containing a sequence corresponding to amino acids 1-358 of human SKAP1 (NP_001068567.1).
Application:WB
Recommended Dilution:WB 1:500 - 1:2000
Reactivity:Mouse, Rat
Positive Samples:Mouse thymus, Rat thymus
Immunogen:Recombinant fusion protein containing a sequence corresponding to amino acids 1-358 of human SKAP1 (NP_001068567.1).
Purification Method:Affinity purification
Storage Buffer:Store at -20'C. Avoid freeze / thaw cycles. Buffer: PBS with 0.02% sodium azide, 50% glycerol, pH7.3.
Isotype:IgG
Sequence:MQAA ALPE EIRW LLED AEEF LAEG LRNE NLSA VARD HRDH ILRG FQQI KARY YWDF QPQG GDIG QDSS DDNH SGTL GLSL TSDA PFLS DYQD EGME DIVK GAQE LDNV IKQG YLEK KSKD HSFF GSEW QKRW CVVS RGLF YYYA NEKS KQPK GTFL IKGY GVRM APHL RRDS KKES CFEL TSQD RRSY EFTA TSPA EARD WVDQ ISFL LKDL SSLT IPYE EDEE EEEK EETY DDID GFDS PSCG SQCR PTIL PGSV GIKE PTEE KEEE DIYE VLPD EEHD LEED ESGT RRKG DYAS YYQG LWDC HGDQ PDEL SFQR GDLI RILS KEYN MYGW WVGE LNSL VGIV PKEY LTTA FEVE ER
Gene ID:8631
Uniprot:Q86WV1
Cellular Location:Cell membrane, Cytoplasm, Nucleus
Calculated MW:41kDa
Observed MW:55kDa
Synonyms:SKAP1, HEL-S-81p, SCAP1, SKAP55
Background:This gene encodes a T cell adaptor protein, a class of intracellular molecules with modular domains capable of recruiting additional proteins but that exhibit no intrinsic enzymatic activity. The encoded protein contains a unique N-terminal region followed by a PH domain and C-terminal SH3 domain. Along with the adhesion and degranulation-promoting adaptor protein, the encoded protein plays a critical role in inside-out signaling by coupling T-cell antigen receptor stimulation to the activation of integrins.
UniProt Protein Function:SKAP55: Positively regulates T-cell receptor signaling by enhancing the MAP kinase pathway. Required for optimal conjugation between T-cells and antigen-presenting cells by promoting the clustering of integrin ITGAL on the surface of T-cells. May be involved in high affinity immunoglobulin epsilon receptor signaling in mast cells. Belongs to the SKAP family. 2 isoforms of the human protein are produced by alternative splicing.
UniProt Protein Details:

Protein type:Adaptor/scaffold; Motility/polarity/chemotaxis

Chromosomal Location of Human Ortholog: 17q21.32

Cellular Component: cytoplasm; plasma membrane; intercellular junction; nucleus

Molecular Function:protein binding; SH3/SH2 adaptor activity; protein complex binding; SH2 domain binding; SH3 domain binding; protein phosphatase binding; protein kinase binding

Biological Process: positive regulation of signal transduction; positive regulation of transcription, DNA-dependent; positive regulation of transcription from RNA polymerase II promoter; T cell receptor signaling pathway

NCBI Summary:This gene encodes a T cell adaptor protein, a class of intracellular molecules with modular domains capable of recruiting additional proteins but that exhibit no intrinsic enzymatic activity. The encoded protein contains a unique N-terminal region followed by a PH domain and C-terminal SH3 domain. Along with the adhesion and degranulation-promoting adaptor protein, the encoded protein plays a critical role in inside-out signaling by coupling T-cell antigen receptor stimulation to the activation of integrins. [provided by RefSeq, Jul 2008]
UniProt Code:Q86WV1
NCBI GenInfo Identifier:269849660
NCBI Gene ID:8631
NCBI Accession:Q86WV1.3
UniProt Related Accession:Q86WV1
Molecular Weight:
NCBI Full Name:Src kinase-associated phosphoprotein 1
NCBI Synonym Full Names:src kinase associated phosphoprotein 1
NCBI Official Symbol:SKAP1  
NCBI Official Synonym Symbols:SCAP1; SKAP55; HEL-S-81p  
NCBI Protein Information:src kinase-associated phosphoprotein 1
UniProt Protein Name:Src kinase-associated phosphoprotein 1
UniProt Synonym Protein Names:Src family-associated phosphoprotein 1; Src kinase-associated phosphoprotein of 55 kDa; SKAP-55; pp55
Protein Family:Src kinase-associated phosphoprotein
UniProt Gene Name:SKAP1  
UniProt Entry Name:SKAP1_HUMAN
Anti-SKAP1 Antibody (CAB3345)Western blot analysis of extracts of various cell lines, using SKAP1 antibody (CAB3345) at 1:3000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (CABS014) at 1:10000 dilution. Lysates/proteins: 25ug per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit (RM00020). Exposure time: 90s.
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