Epigenetics & Nuclear Signaling Antibodies 2

Anti-SIX5 Antibody (CAB15960)

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SKU:
CAB15960
Product Type:
Antibody
Reactivity:
Mouse
Reactivity:
Rat
Host Species:
Rabbit
Isotype:
IgG
Antibody Type:
Polyclonal Antibody
Research Area:
Epigenetics and Nuclear Signaling

Description

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Antibody Name:Anti-SIX5 Antibody
Antibody SKU:CAB15960
Antibody Size:20uL, 50uL, 100uL
Application:WB
Reactivity:Mouse, Rat
Host Species:Rabbit
Immunogen:Recombinant fusion protein containing a sequence corresponding to amino acids 1-170 of human SIX5 (NP_787071.2).
Application:WB
Recommended Dilution:WB 1:500 - 1:2000
Reactivity:Mouse, Rat
Positive Samples:Mouse eye, Rat eye
Immunogen:Recombinant fusion protein containing a sequence corresponding to amino acids 1-170 of human SIX5 (NP_787071.2).
Purification Method:Affinity purification
Storage Buffer:Store at -20'C. Avoid freeze / thaw cycles. Buffer: PBS with 0.02% sodium azide, 50% glycerol, pH7.3.
Isotype:IgG
Sequence:MATL PAEP SAGP AAGG EAVA AAAA TEEE EEEA RQLL QTLQ AAEG EAAA AAGA GAGA AAAG AEGP GSPG VPGS PPEA ASEP PTGL RFSP EQVA CVCE ALLQ AGHA GRLS RFLG ALPP AERL RGSD PVLR ARAL VAFQ RGEY AELY RLLE SRPF PAAH HAFL QDLY LRAR YH
Gene ID:147912
Uniprot:Q8N196
Cellular Location:Cytoplasm, Nucleus
Calculated MW:74kDa
Observed MW:74kDa
Synonyms:SIX5, BOR2, DMAHP
Background:The protein encoded by this gene is a homeodomain-containing transcription factor that appears to function in the regulation of organogenesis. This gene is located downstream of the dystrophia myotonica-protein kinase gene. Mutations in this gene are a cause of branchiootorenal syndrome type 2.
UniProt Protein Function:SIX5: Transcription factor that is thought to be involved in regulation of organogenesis. May be involved in determination and maintenance of retina formation. Binds a 5'-GGTGTCAG-3' motif present in the ARE regulatory element of ATP1A1. Binds a 5'- TCA[AG][AG]TTNC-3' motif present in the MEF3 element in the myogenin promoter, and in the IGFBP5 promoter. Thought to be regulated by association with Dach and Eya proteins, and seems to be coactivated by EYA1, EYA2 and EYA3. Defects in SIX5 are the cause of branchiootorenal syndrome type 2 (BOR2). BOR is an autosomal dominant disorder manifested by various combinations of preauricular pits, branchial fistulae or cysts, lacrimal duct stenosis, hearing loss, structural defects of the outer, middle, or inner ear, and renal dysplasia. Associated defects include asthenic habitus, long narrow facies, constricted palate, deep overbite, and myopia. Hearing loss may be due to mondini type cochlear defect and stapes fixation. Penetrance of BOR syndrome is high, although expressivity can be extremely variable. Belongs to the SIX/Sine oculis homeobox family.
UniProt Protein Details:

Protein type:Transcription factor; DNA-binding

Chromosomal Location of Human Ortholog: 19q13.32

Cellular Component: cytoplasm; Golgi apparatus; intracellular membrane-bound organelle; nucleoplasm

Molecular Function:protein binding

Disease: Branchiootorenal Syndrome 2

NCBI Summary:The protein encoded by this gene is a homeodomain-containing transcription factor that appears to function in the regulation of organogenesis. This gene is located downstream of the dystrophia myotonica-protein kinase gene. Mutations in this gene are a cause of branchiootorenal syndrome type 2. [provided by RefSeq, Jul 2009]
UniProt Code:Q8N196
NCBI GenInfo Identifier:150421671
NCBI Gene ID:147912
NCBI Accession:Q8N196.3
UniProt Related Accession:Q8N196
Molecular Weight:74,562 Da
NCBI Full Name:Homeobox protein SIX5
NCBI Synonym Full Names:SIX homeobox 5
NCBI Official Symbol:SIX5  
NCBI Official Synonym Symbols:BOR2; DMAHP  
NCBI Protein Information:homeobox protein SIX5
UniProt Protein Name:Homeobox protein SIX5
UniProt Synonym Protein Names:DM locus-associated homeodomain protein; Sine oculis homeobox homolog 5
Protein Family:Homeobox protein
UniProt Gene Name:SIX5  
UniProt Entry Name:SIX5_HUMAN
Anti-SIX5 Antibody (CAB15960)Western blot analysis of extracts of various cell lines, using SIX5 antibody (CAB15960) at 1000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (CABS014) at 1:10000 dilution. Lysates/proteins: 25ug per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit (RM00020). Exposure time: 1s.
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