Name: Anti-SIX2 Antibody (CAB8727)
Brand: Cell Biology Antibodies 12
SKU: CAB8727
Availability: OutOfStock

Antibody Name:	Anti-SIX2 Antibody
Antibody SKU:	CAB8727
Antibody Size:	20uL, 50uL, 100uL
Application:	WB
Reactivity:	Human, Rat
Host Species:	Rabbit
Immunogen:	A synthetic peptide corresponding to a sequence within amino acids 200 to the C-terminus of human SIX2 (NP_058628.3).

Application:	WB
Recommended Dilution:	WB 1:200 - 1:2000
Reactivity:	Human, Rat
Positive Samples:	Rat kidney

Immunogen:	A synthetic peptide corresponding to a sequence within amino acids 200 to the C-terminus of human SIX2 (NP_058628.3).
Purification Method:	Affinity purification
Storage Buffer:	Store at -20'C. Avoid freeze / thaw cycles. Buffer: PBS with 0.02% sodium azide, 50% glycerol, pH7.3.
Isotype:	IgG
Sequence:	PLNG SGKS VLGS SEDE KTPS GTPD HSSS SPAL LLSP PPPG LPSL HSLG HPPG PSAV PVPV PGGG GADP LQHH HGLQ DSIL NPMS ANLV DLGS
Gene ID:	10736
Uniprot:	Q9NPC8
Cellular Location:	Nucleus
Calculated MW:	32kDa
Observed MW:	32kDa

Synonyms:	SIX2
Background:	This gene is a member of the vertebrate gene family which encode proteins homologous to the Drosophila 'sine oculis' homeobox protein. The encoded protein is a transcription factor which, like other members of this gene family, may be involved in limb or eye development.

UniProt Protein Function:	SIX2: Transcription factor that plays an important role in the development of several organs, including kidney, skull and stomach. During kidney development, maintains cap mesenchyme multipotent nephron progenitor cells in an undifferentiated state by opposing the inductive signals emanating from the ureteric bud and cooperates with WNT9B to promote renewing progenitor cells proliferation. Acts through its interaction with TCF7L2 and OSR1 in a canonical Wnt signaling independent manner preventing transcription of differentiation genes in cap mesenchyme such as WNT4. Also acts independently of OSR1 to activate expression of many cap mesenchyme genes, including itself, GDNF and OSR1. During craniofacial development plays a role in growth and elongation of the cranial base through regulation of chondrocyte differentiation. During stomach organogenesis, controls pyloric sphincter formation and mucosal growth through regulation of a gene network including NKX2-5, BMPR1B, BMP4, SOX9 and GREM1. During branchial arch development, acts to mediate HOXA2 control over the insulin-like growth factor pathway. Also may be involved in limb tendon and ligament development. Plays a role in cell proliferation and migration. {ECO:0000250\|UniProtKB:Q62232, ECO:0000269\|PubMed:22995329}. Belongs to the SIX/Sine oculis homeobox family. {ECO:0000305}. Interacts with TCF7L2; in a canonical Wnt signaling independent manner; prevents transcription of differentiation genes in cap mesenchyme. Interacts with OSR1; form a strong repressor complex with TCF7L2, TLE2 and TLE3 to prevent the activation of Wnt/beta-catenin target genes in the cap mesenchyme. Interacts with HOXA11, EYA1 and EYA3. {ECO:0000250\|UniProtKB:Q62232}
UniProt Protein Details:	Protein type:DNA-binding Chromosomal Location of Human Ortholog: 2p21 Cellular Component: cytoplasm; nuclear membrane; nucleoplasm; nucleus; plasma membrane Biological Process: anatomical structure morphogenesis; anterior/posterior axis specification; cell migration; cell proliferation; embryonic digestive tract morphogenesis; kidney development; mesodermal cell fate specification
NCBI Summary:	This gene is a member of the vertebrate gene family which encode proteins homologous to the Drosophila 'sine oculis' homeobox protein. The encoded protein is a transcription factor which, like other members of this gene family, may be involved in limb or eye development. [provided by RefSeq, Dec 2008]
UniProt Code:	Q9NPC8
NCBI GenInfo Identifier:	12230598
NCBI Gene ID:	10736
NCBI Accession:	Q9NPC8.1
UniProt Secondary Accession:	Q9NPC8,Q9BXH7,
UniProt Related Accession:	Q9NPC8
Molecular Weight:	32,286 Da
NCBI Full Name:	Homeobox protein SIX2
NCBI Synonym Full Names:	SIX homeobox 2
NCBI Official Symbol:	SIX2
NCBI Protein Information:	homeobox protein SIX2
UniProt Protein Name:	Homeobox protein SIX2
UniProt Synonym Protein Names:	Sine oculis homeobox homolog 2
Protein Family:	Homeobox protein
UniProt Gene Name:	SIX2
UniProt Entry Name:	SIX2_HUMAN

Western blot analysis of extracts of Rat kidney, using SIX2 antibody (CAB8727) at 1:1000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (CABS014) at 1:10000 dilution. Lysates/proteins: 25ug per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Enhanced Kit (RM00021). Exposure time: 10s.

Antibodies

SIX2 Antibody (PACO03366)

SIX2 Antibody (PACO12226)

Secondary Antibody

Anti-HRP Goat Anti-Rabbit IgG (H+L) Antibody (CABS014)