Antibody Name:	Anti-SIRT4 Antibody
Antibody SKU:	CAB7585
Antibody Size:	20uL, 50uL, 100uL
Application:	WB IHC
Reactivity:	Human, Mouse, Rat
Host Species:	Rabbit
Immunogen:	Recombinant fusion protein containing a sequence corresponding to amino acids 29-260 of human SIRT4 (NP_036372.1).

Application:	WB IHC
Recommended Dilution:	WB 1:500 - 1:2000 IHC 1:50 - 1:200
Reactivity:	Human, Mouse, Rat
Positive Samples:	Mouse testis, Rat testis

Immunogen:	Recombinant fusion protein containing a sequence corresponding to amino acids 29-260 of human SIRT4 (NP_036372.1).
Purification Method:	Affinity purification
Storage Buffer:	Store at -20'C. Avoid freeze / thaw cycles. Buffer: PBS with 0.02% sodium azide, 50% glycerol, pH7.3.
Isotype:	IgG
Sequence:	IGLF VPAS PPLD PEKV KELQ RFIT LSKR LLVM TGAG ISTE SGIP DYRS EKVG LYAR TDRR PIQH GDFV RSAP IRQR YWAR NFVG WPQF SSHQ PNPA HWAL STWE KLGK LYWL VTQN VDAL HTKA GSRR LTEL HGCM DRVL CLDC GEQT PRGV LQER FQVL NPTW SAEA HGLA PDGD VFLS EEQV RSFQ VPTC VQCG GHLK PDVV FFGD TVNP DKVD FVHK RVKE ADSL LVVG
Gene ID:	23409
Uniprot:	Q9Y6E7
Cellular Location:	Mitochondrion matrix
Calculated MW:	35kDa
Observed MW:	32KDa

Synonyms:

SIRT4, SIR2L4, sirtuin 4

Background:

This gene encodes a member of the sirtuin family of proteins, homologs to the yeast Sir2 protein. Members of the sirtuin family are characterized by a sirtuin core domain and grouped into four classes. The functions of human sirtuins have not yet been determined; however, yeast sirtuin proteins are known to regulate epigenetic gene silencing and suppress recombination of rDNA. Studies suggest that the human sirtuins may function as intracellular regulatory proteins with mono-ADP-ribosyltransferase activity. The protein encoded by this gene is included in class IV of the sirtuin family.

UniProt Protein Function:	SIRT4: NAD-dependent protein ADP-ribosyl transferase. Catalyzes the transfer of ADP-ribosyl groups onto target proteins, including mitochondrial GLUD1. Inhibits GLUD1 enzyme activity. Down- regulates insulin secretion. Has no detectable protein deacetylase activity. Belongs to the sirtuin family. Class II subfamily.
UniProt Protein Details:	Protein type:Tumor suppressor; Mitochondrial; Deacetylase; EC 2.4.2.-; EC 3.5.1.- Chromosomal Location of Human Ortholog: 12q Cellular Component: mitochondrial matrix; mitochondrion Molecular Function:biotinidase activity; NAD+ ADP-ribosyltransferase activity; protein binding Biological Process: chromatin silencing; glutamine metabolic process; negative regulation of fatty acid oxidation; negative regulation of insulin secretion; positive regulation of lipid biosynthetic process; protein amino acid ADP-ribosylation; response to DNA damage stimulus
NCBI Summary:	This gene encodes a member of the sirtuin family of proteins, homologs to the yeast Sir2 protein. Members of the sirtuin family are characterized by a sirtuin core domain and grouped into four classes. The functions of human sirtuins have not yet been determined; however, yeast sirtuin proteins are known to regulate epigenetic gene silencing and suppress recombination of rDNA. Studies suggest that the human sirtuins may function as intracellular regulatory proteins with mono-ADP-ribosyltransferase activity. The protein encoded by this gene is included in class IV of the sirtuin family. [provided by RefSeq, Jul 2008]
UniProt Code:	Q9Y6E7
NCBI GenInfo Identifier:	38258657
NCBI Gene ID:	23409
NCBI Accession:	Q9Y6E7.1
UniProt Secondary Accession:	Q9Y6E7,O43346, Q32M33,
UniProt Related Accession:	Q9Y6E7
Molecular Weight:	35,188 Da
NCBI Full Name:	NAD-dependent protein lipoamidase sirtuin-4, mitochondrial
NCBI Synonym Full Names:	sirtuin 4
NCBI Official Symbol:	SIRT4
NCBI Official Synonym Symbols:	SIR2L4
NCBI Protein Information:	NAD-dependent protein lipoamidase sirtuin-4, mitochondrial
UniProt Protein Name:	NAD-dependent protein lipoamidase sirtuin-4, mitochondrial
UniProt Synonym Protein Names:	NAD-dependent ADP-ribosyltransferase sirtuin-4
Protein Family:	NAD-dependent protein deacylase
UniProt Gene Name:	SIRT4
UniProt Entry Name:	SIR4_HUMAN

	Western blot analysis of extracts of various cell lines, using SIRT4 Rabbit pAb (CAB7585) at 1:1000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (CABS014) at 1:10000 dilution. Lysates/proteins: 25ug per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit (RM00020). Exposure time: 180s.
	Immunohistochemistry of paraffin-embedded human colon using SIRT4 Rabbit pAb (CAB7585) at dilution of 1:100 (40x lens).
	Immunohistochemistry of paraffin-embedded mouse kidney using SIRT4 Rabbit pAb (CAB7585) at dilution of 1:100 (40x lens).
	Immunohistochemistry of paraffin-embedded rat brain using SIRT4 Rabbit pAb (CAB7585) at dilution of 1:100 (40x lens).