KO Validated Antibodies 2

Anti-SIPA1 Antibody (CAB19867)[KO Validated]

(No reviews yet) Write a Review
SKU:
CAB19867
Product Type:
Antibody
Reactivity:
Human
Reactivity:
Mouse
Host Species:
Rabbit
Isotype:
IgG
Research Area:
Cell Biology

Description

system_update_altDatasheetsystem_update_altMSDS
Antibody Name:Anti-SIPA1 Antibody [KO Validated]
Antibody SKU:CAB19867
Antibody Size:20uL, 50uL, 100uL
Application:WB IHC
Reactivity:Human, Mouse
Host Species:Rabbit
Immunogen:Recombinant protein of human SIPA1.
Application:WB IHC
Recommended Dilution:WB 1:500 - 1:2000 IHC 1:100 - 1:200
Reactivity:Human, Mouse
Positive Samples:HeLa
Immunogen:Recombinant protein of human SIPA1.
Purification Method:Affinity purification
Storage Buffer:Store at -20°C. Avoid freeze / thaw cycles. Buffer: PBS with 0.02% sodium azide, 50% glycerol, pH7.3.
Isotype:IgG
Sequence:Email for sequence
Gene ID:6494
Uniprot:Q96FS4
Cellular Location:Cytoplasm, Endomembrane system, Nucleus, Peripheral membrane protein, perinuclear region
Calculated MW:112kDa
Observed MW:130KDa
Anti-SIPA1 Antibody [KO Validated] (CAB19867)Western blot analysis of extracts from normal (control) and SIPA1 knockout (KO) HeLa cells, using SIPA1 antibody (CAB19867) at 1:3000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (CABS014) at 1:10000 dilution. Lysates/proteins: 25ug per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit (RM00020). Exposure time: 1s.
Synonyms:SIPA1, SPA1
Background:The product of this gene is a mitogen induced GTPase activating protein (GAP). It exhibits a specific GAP activity for Ras-related regulatory proteins Rap1 and Rap2, but not for Ran or other small GTPases. This protein may also hamper mitogen-induced cell cycle progression when abnormally or prematurely expressed. It is localized to the perinuclear region. Two alternatively spliced variants encoding the same isoform have been characterized to date.
UniProt Protein Function:SIPA1: GTPase activator for the nuclear Ras-related regulatory proteins Rap1 and Rap2 in vitro, converting it to the putatively inactive GDP-bound state.
UniProt Protein Details:

Protein type:GAPs; GAPs, Ras

Chromosomal Location of Human Ortholog: 11q13

Cellular Component: cytosol; nucleus

Molecular Function:GTPase activator activity; protein binding

Biological Process: cell proliferation; signal transduction

NCBI Summary:The product of this gene is a mitogen induced GTPase activating protein (GAP). It exhibits a specific GAP activity for Ras-related regulatory proteins Rap1 and Rap2, but not for Ran or other small GTPases. This protein may also hamper mitogen-induced cell cycle progression when abnormally or prematurely expressed. It is localized to the perinuclear region. Two alternatively spliced variants encoding the same isoform have been characterized to date. [provided by RefSeq, Jul 2008]
UniProt Code:Q96FS4
NCBI GenInfo Identifier:20455286
NCBI Gene ID:6494
NCBI Accession:Q96FS4.1
UniProt Secondary Accession:Q96FS4,O14518, O60484, O60618, Q2YD83,
UniProt Related Accession:Q96FS4
Molecular Weight:112,149 Da
NCBI Full Name:Signal-induced proliferation-associated protein 1
NCBI Synonym Full Names:signal-induced proliferation-associated 1
NCBI Official Symbol:SIPA1  
NCBI Official Synonym Symbols:SPA1  
NCBI Protein Information:signal-induced proliferation-associated protein 1
UniProt Protein Name:Signal-induced proliferation-associated protein 1
UniProt Synonym Protein Names:GTPase-activating protein Spa-1; p130 SPA-1
Protein Family:Signal-induced proliferation-associated protein
UniProt Gene Name:SIPA1  
UniProt Entry Name:SIPA1_HUMAN
View AllClose

0 Reviews

View AllClose

Related Products