Cell Biology Antibodies 2
Anti-SIPA1 Antibody (CAB12253)
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- SKU:
- CAB12253
- Product Type:
- Antibody
- Reactivity:
- Human
- Reactivity:
- Mouse
- Host Species:
- Rabbit
- Isotype:
- IgG
- Antibody Type:
- Polyclonal Antibody
- Research Area:
- Cell Biology
Description
| Antibody Name: | Anti-SIPA1 Antibody |
| Antibody SKU: | CAB12253 |
| Antibody Size: | 20uL, 50uL, 100uL |
| Application: | WB IHC |
| Reactivity: | Human, Mouse |
| Host Species: | Rabbit |
| Immunogen: | Recombinant fusion protein containing a sequence corresponding to amino acids 763-1042 of human SIPA1 (NP_694985.29). |
| Application: | WB IHC |
| Recommended Dilution: | WB 1:500 - 1:2000 IHC 1:100 - 1:200 |
| Reactivity: | Human, Mouse |
| Positive Samples: | Raji, K-562, HeLa, LO2, Mouse spleen |
| Immunogen: | Recombinant fusion protein containing a sequence corresponding to amino acids 763-1042 of human SIPA1 (NP_694985.29). |
| Purification Method: | Affinity purification |
| Storage Buffer: | Store at -20'C. Avoid freeze / thaw cycles. Buffer: PBS with 0.02% sodium azide, 50% glycerol, pH7.3. |
| Isotype: | IgG |
| Sequence: | ESGR PRRS FSEL YTLS LQEP SRRG APDP VQDE VQGV TLLP TTKQ LLHL CLQD GGSP PGPG DLAE ERTE FLHS QNSL SPRS SLSD EAPV LPNT TPDL LLAT TAKP SVPS ADSE TPLT QDRP GSPS GSED KGNP APEL RASF LPRT LSLR NSIS RIMS EAGS GTLE DEWQ AISE IAST CNTI LESL SREG QPIP ESGD PKGT PKSD AEPE PGNL SEKV SHLE SMLR KLQE DLQK EKAD RAAL EEEV RSLR HNNR RLQA ESES AATR LLLA SKQL GSPT ADLA |
| Gene ID: | 6494 |
| Uniprot: | Q96FS4 |
| Cellular Location: | Cytoplasm, Endomembrane system, Nucleus, Peripheral membrane protein, perinuclear region |
| Calculated MW: | 112kDa |
| Observed MW: | 130kDa |
| Synonyms: | SIPA1, SPA1 |
| Background: | The product of this gene is a mitogen induced GTPase activating protein (GAP). It exhibits a specific GAP activity for Ras-related regulatory proteins Rap1 and Rap2, but not for Ran or other small GTPases. This protein may also hamper mitogen-induced cell cycle progression when abnormally or prematurely expressed. It is localized to the perinuclear region. Two alternatively spliced variants encoding the same isoform have been characterized to date. |
| UniProt Protein Function: | SIPA1: GTPase activator for the nuclear Ras-related regulatory proteins Rap1 and Rap2 in vitro, converting it to the putatively inactive GDP-bound state. |
| UniProt Protein Details: | Protein type:GAPs; GAPs, Ras Chromosomal Location of Human Ortholog: 11q13 Cellular Component: cytosol; nucleus Molecular Function:GTPase activator activity; protein binding Biological Process: cell proliferation; signal transduction |
| NCBI Summary: | The product of this gene is a mitogen induced GTPase activating protein (GAP). It exhibits a specific GAP activity for Ras-related regulatory proteins Rap1 and Rap2, but not for Ran or other small GTPases. This protein may also hamper mitogen-induced cell cycle progression when abnormally or prematurely expressed. It is localized to the perinuclear region. Two alternatively spliced variants encoding the same isoform have been characterized to date. [provided by RefSeq, Jul 2008] |
| UniProt Code: | Q96FS4 |
| NCBI GenInfo Identifier: | 20455286 |
| NCBI Gene ID: | 6494 |
| NCBI Accession: | Q96FS4.1 |
| UniProt Secondary Accession: | Q96FS4,O14518, O60484, O60618, Q2YD83, |
| UniProt Related Accession: | Q96FS4 |
| Molecular Weight: | 112,149 Da |
| NCBI Full Name: | Signal-induced proliferation-associated protein 1 |
| NCBI Synonym Full Names: | signal-induced proliferation-associated 1 |
| NCBI Official Symbol: | SIPA1 |
| NCBI Official Synonym Symbols: | SPA1 |
| NCBI Protein Information: | signal-induced proliferation-associated protein 1 |
| UniProt Protein Name: | Signal-induced proliferation-associated protein 1 |
| UniProt Synonym Protein Names: | GTPase-activating protein Spa-1; p130 SPA-1 |
| Protein Family: | Signal-induced proliferation-associated protein |
| UniProt Gene Name: | SIPA1 |
| UniProt Entry Name: | SIPA1_HUMAN |
 | Western blot analysis of extracts of various cell lines, using SIPA1 antibody (CAB12253) at 1:3000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (CABS014) at 1:10000 dilution. Lysates/proteins: 25ug per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit (RM00020). Exposure time: 90s. |
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