Epigenetics & Nuclear Signaling Antibodies 1

Anti-SIN3B Antibody (CAB10584)

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SKU:
CAB10584
Product Type:
Antibody
Reactivity:
Human
Reactivity:
Mouse
Host Species:
Rabbit
Isotype:
IgG
Antibody Type:
Polyclonal Antibody
Research Area:
Epigenetics and Nuclear Signaling

Description

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Antibody Name:Anti-SIN3B Antibody
Antibody SKU:CAB10584
Antibody Size:20uL, 50uL, 100uL
Application:WB
Reactivity:Human, Mouse
Host Species:Rabbit
Immunogen:Recombinant fusion protein containing a sequence corresponding to amino acids 1-160 of human SIN3B (NP_056075.1).
Application:WB
Recommended Dilution:WB 1:1000 - 1:2000
Reactivity:Human, Mouse
Positive Samples:A-431, Raji, HeLa, Mouse kidney, Mouse testis, Mouse brain
Immunogen:Recombinant fusion protein containing a sequence corresponding to amino acids 1-160 of human SIN3B (NP_056075.1).
Purification Method:Affinity purification
Storage Buffer:Store at -20'C. Avoid freeze / thaw cycles. Buffer: PBS with 0.02% sodium azide, 50% glycerol, pH7.3.
Isotype:IgG
Sequence:MAHA GGGS GGSG AGGP AGRG LSGA RWGR SGSA GHEK LPVH VEDA LTYL DQVK IRFG SDPA TYNG FLEI MKEF KSQS IDTP GVIR RVSQ LFHE HPDL IVGF NAFL PLGY RIDI PKNG KLNI QSPL TSQE NSHN HGDG AEDF KQQV PYKE DKPQ VPLE SDSV
Gene ID:23309
Uniprot:O75182
Cellular Location:Nucleus
Calculated MW:39kDa/129kDa/133kDa
Observed MW:133kDa
Synonyms:SIN3B
Background:
UniProt Protein Function:SIN3B: Acts as a transcriptional repressor. Interacts with MXI1 to repress MYC responsive genes and antagonize MYC oncogenic activities. Interacts with MAD-MAX heterodimers by binding to MAD. The heterodimer then represses transcription by tethering SIN3B to DNA. Also forms a complex with FOXK1 which represses transcription. 3 isoforms of the human protein are produced by alternative splicing.
UniProt Protein Details:

Protein type:Nuclear receptor co-regulator; Transcription, coactivator/corepressor

Chromosomal Location of Human Ortholog: 19p13.11

Cellular Component: nucleoplasm; nucleus; Sin3 complex

Molecular Function:histone deacetylase activity; protein binding

Biological Process: cellular lipid metabolic process; histone deacetylation; negative regulation of transcription from RNA polymerase II promoter

UniProt Code:O75182
NCBI GenInfo Identifier:68067946
NCBI Gene ID:23309
NCBI Accession:O75182.2
UniProt Secondary Accession:O75182,Q2NL91, Q68GC2, Q6P4B8, Q8TB34, Q9BSC8,
UniProt Related Accession:O75182
Molecular Weight:133kDa
NCBI Full Name:Paired amphipathic helix protein Sin3b
NCBI Synonym Full Names:SIN3 transcription regulator family member B
NCBI Official Symbol:SIN3B  
NCBI Protein Information:paired amphipathic helix protein Sin3b
UniProt Protein Name:Paired amphipathic helix protein Sin3b
UniProt Synonym Protein Names:Histone deacetylase complex subunit Sin3b; Transcriptional corepressor Sin3b
Protein Family:Paired amphipathic helix protein
UniProt Gene Name:SIN3B  
Anti-SIN3B Antibody (CAB10584)Western blot analysis of extracts of various cell lines, using SIN3B antibody (CAB10584) at 1:1000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (CABS014) at 1:10000 dilution. Lysates/proteins: 25ug per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit (RM00020). Exposure time: 60s.
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