Cell Biology Antibodies 12
Anti-SIK2 Antibody (CAB8321)
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- SKU:
- CAB8321
- Product Type:
- Antibody
- Applications:
- WB
- Reactivity:
- Human
- Reactivity:
- Mouse
- Reactivity:
- Rat
- Host Species:
- Rabbit
- Isotype:
- IgG
- Research Area:
- Cell Biology
Description
| Antibody Name: | SIK2 Rabbit Polyclonal Antibody |
| Antibody SKU: | CAB8321 |
| Antibody Size: | 20uL, 50uL, 100uL |
| Application: | WB |
| Reactivity: | Human, Mouse, Rat |
| Host Species: | Rabbit |
| Immunogen: | Recombinant fusion protein containing a sequence corresponding to amino acids 827-926 of human SIK2 (NP_056006.1). |
| Application: | WB |
| Recommended Dilution: | WB 1:500 - 1:2000 |
| Reactivity: | Human, Mouse, Rat |
| Positive Samples: | U-87MG, SW620, A-549, HeLa, Jurkat, HepG2, Mouse fat, Mouse testis, Rat testis |
| Immunogen: | Recombinant fusion protein containing a sequence corresponding to amino acids 827-926 of human SIK2 (NP_056006.1). |
| Purification Method: | Affinity purification |
| Storage Buffer: | Store at -20'C. Avoid freeze / thaw cycles. Buffer: PBS with 0.02% sodium azide, 50% glycerol, pH7.3. |
| Isotype: | IgG |
| Sequence: | PPPP PPRQ PGAA PAPL QFSY QTCE LPSA ASPA PDYP TPCQ YPVD GAQQ SDLT GPDC PRSP GLQE APSS YDPL ALSE LPGL FDCE MLDA VDPQ HNGY VLVN |
| Gene ID: | 23235 |
| Uniprot: | Q9H0K1 |
| Cellular Location: | Cytoplasm |
| Calculated MW: | 103kDa |
| Observed MW: | 129kDa |
| Synonyms: | SIK2, LOH11CR1I, QIK, SIK-2, SNF1LK2 |
| Background: |
| UniProt Protein Function: | QIK: a serine/threonine kinase of the CAMKL family and related to AMPK. Specifically expressed in adipose tissues. Like AMPK, QIK is phosphorylated and activated by LKB1. Part of a molecular complex including TORC2 and calcineurin that regulates the effects of circulating glucose and gut hormones during feeding on TORC2-mediated gene expression. In mice, insulin promotes the phosphorylation and ubiquitin-dependent degradation of TORC2, inhibiting gluconeogenic gene expression during re-feeding. In response to insulin, Akt2 phosphorylates and activates QIK which in turn stimulates the phosphorylation and cytoplasmic translocation of TORC2 where it associates with COP1, which promotes its ubiquitination and proteasomal degradation. Phosphorylates Ser794 of IRS1 in insulin-stimulated adipocytes, potentially modulating the efficiency of insulin signal transduction. Inhibits CREB activity by phosphorylating and repressing the CREB-specific coactivators, CRTC1-3. |
| UniProt Protein Details: | Protein type:Protein kinase, CAMK; Protein kinase, Ser/Thr (non-receptor); EC 2.7.11.1; Kinase, protein; CAMK group; CAMKL family; QIK subfamily Chromosomal Location of Human Ortholog: 11q23.1 Cellular Component: cytoplasm; nucleus Molecular Function:ATP binding; magnesium ion binding; protein binding; protein serine/threonine kinase activity Biological Process: insulin receptor signaling pathway; protein amino acid autophosphorylation; protein amino acid phosphorylation; regulation of insulin receptor signaling pathway |
| UniProt Code: | Q9H0K1 |
| NCBI GenInfo Identifier: | 59798973 |
| NCBI Gene ID: | 23235 |
| NCBI Accession: | Q9H0K1.1 |
| UniProt Secondary Accession: | Q9H0K1,O94878, Q17RV0, Q6AZE2, Q76N03, Q8NCV7, Q96CZ8 A8K5B8, B0YJ94, |
| UniProt Related Accession: | Q9H0K1 |
| Molecular Weight: | 103,915 Da |
| NCBI Full Name: | Serine/threonine-protein kinase SIK2 |
| NCBI Synonym Full Names: | salt inducible kinase 2 |
| NCBI Official Symbol: | SIK2 |
| NCBI Official Synonym Symbols: | QIK; SIK-2; SNF1LK2; LOH11CR1I |
| NCBI Protein Information: | serine/threonine-protein kinase SIK2 |
| UniProt Protein Name: | Serine/threonine-protein kinase SIK2 |
| UniProt Synonym Protein Names: | Qin-induced kinase; Salt-inducible kinase 2; SIK-2; Serine/threonine-protein kinase SNF1-like kinase 2 |
| Protein Family: | Serine/threonine-protein kinase |
| UniProt Gene Name: | SIK2 |
| UniProt Entry Name: | SIK2_HUMAN |
 | Western blot analysis of extracts of various cell lines, using SIK2 antibody (CAB8321) at 1:1000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (CABS014) at 1:10000 dilution. Lysates/proteins: 25ug per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit (RM00020). Exposure time: 30s. |
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