Cell Biology Antibodies 5

Anti-SIK1 Antibody (CAB15247)

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SKU:
CAB15247
Product Type:
Antibody
Reactivity:
Mouse
Host Species:
Rabbit
Isotype:
IgG
Antibody Type:
Polyclonal Antibody
Research Area:
Cell Biology

Description

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Antibody Name:Anti-SIK1 Antibody
Antibody SKU:CAB15247
Antibody Size:20uL, 50uL, 100uL
Application:WB
Reactivity:Mouse
Host Species:Rabbit
Immunogen:A synthetic peptide corresponding to a sequence within amino acids 1-100 of human SIK1 (NP_775490.2).
Application:WB
Recommended Dilution:WB 1:500 - 1:2000
Reactivity:Mouse
Positive Samples:
Immunogen:A synthetic peptide corresponding to a sequence within amino acids 1-100 of human SIK1 (NP_775490.2).
Purification Method:Affinity purification
Storage Buffer:Store at -20'C. Avoid freeze / thaw cycles. Buffer: PBS with 0.02% sodium azide, 50% glycerol, pH7.3.
Isotype:IgG
Sequence:MVIM SEFS ADPA GQGQ GQQK PLRV GFYD IERT LGKG NFAV VKLA RHRV TKTQ VAIK IIDK TRLD SSNL EKIY REVQ LMKL LNHP HIIK LYQV METK DMLY
Gene ID:150094
Uniprot:P57059
Cellular Location:Cytoplasm, Nucleus
Calculated MW:84kDa
Observed MW:Refer to figures
Synonyms:SIK1, MSK, SIK, SIK-1, SNF1LK, EIEE30
Background:
UniProt Protein Function:SIK: Serine/threonine-protein kinase involved in various processes such as cell cycle regulation, gluconeogenesis and lipogenesis regulation, muscle growth and differentiation and tumor suppression. Phosphorylates HDAC4, HDAC5, PPME1, SREBF1, TORC1/CRTC1 and TORC2/CRTC2. Acts as a tumor suppressor and plays a key role in p53/TP53-dependent anoikis, a type of apoptosis triggered by cell detachment: required for phosphorylation of p53/TP53 in response to loss of adhesion and is able to suppress metastasis. Part of a sodium-sensing signaling network, probably by mediating phosphorylation of PPME1: following increases in intracellular sodium, SIK1 is activated by CaMK1 and phosphorylates PPME1 subunit of protein phosphatase 2A (PP2A), leading to dephosphorylation of sodium/potassium-transporting ATPase ATP1A1 and subsequent increase activity of ATP1A1. Acts as a regulator of muscle cells by phosphorylating and inhibiting class II histone deacetylases HDAC4 and HDAC5, leading to promote expression of MEF2 target genes in myocytes. Also required during cardiomyogenesis by regulating the exit of cardiomyoblasts from the cell cycle via down-regulation of CDKN1C/p57Kip2. Acts as a regulator of hepatic gluconeogenesis by phosphorylating and repressing the CREB-specific coactivators TORC1/CRTC1 and TORC2/CRTC2, leading to inhibit CREB activity. Also regulates hepatic lipogenesis by phosphorylating and inhibiting SREBF1. Interacts with ATP1A1. Interacts (when phosphorylated on Thr-182 and Ser-186) with YWHAZ. Activated by phosphorylation on Thr-182. Also activated by phosphorylation on Thr-322 in response to increases in intracellular sodium in parallel with elevations in intracellular calcium through the reversible sodium/calcium exchanger. Belongs to the protein kinase superfamily. CAMK Ser/Thr protein kinase family. AMPK subfamily.
UniProt Protein Details:

Protein type:EC 2.7.11.1; Protein kinase, Ser/Thr (non-receptor); Tumor suppressor; Kinase, protein; Protein kinase, CAMK; Motility/polarity/chemotaxis; CAMK group; CAMKL family; QIK subfamily

Chromosomal Location of Human Ortholog: 21q22.3

Cellular Component: cytoplasm; nucleus

Molecular Function:ATP binding; cAMP response element binding protein binding; magnesium ion binding; protein binding; protein kinase binding; protein serine/threonine kinase activity

Biological Process: cardiac muscle cell differentiation; cellular response to hormone stimulus; entrainment of circadian clock by photoperiod; inhibition of CREB transcription factor; negative regulation of gluconeogenesis; protein amino acid autophosphorylation; protein amino acid phosphorylation; regulation of cell differentiation; regulation of mitotic cell cycle; regulation of sodium ion transport

Disease: Epileptic Encephalopathy, Early Infantile, 30

UniProt Code:P57059
NCBI GenInfo Identifier:59803093
NCBI Gene ID:102724428
NCBI Accession:P57059.2
UniProt Secondary Accession:P57059,Q5R2V5, Q6ZNL8, Q86YJ2, A6NC84,
Molecular Weight:84,902 Da
NCBI Full Name:Serine/threonine-protein kinase SIK1
NCBI Official Symbol:LOC102724428  
NCBI Protein Information:serine/threonine-protein kinase SIK1
UniProt Protein Name:Serine/threonine-protein kinase SIK1
UniProt Synonym Protein Names:Salt-inducible kinase 1; SIK-1; Serine/threonine-protein kinase SNF1-like kinase 1; Serine/threonine-protein kinase SNF1LK
Protein Family:Serine/threonine-protein kinase
UniProt Gene Name:SIK1  
UniProt Entry Name:SIK1_HUMAN
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