Cell Biology Antibodies 2

Anti-SIGLEC8 Antibody (CAB10514)

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SKU:
CAB10514
Product Type:
Antibody
Reactivity:
Human
Reactivity:
Mouse
Reactivity:
Rat
Host Species:
Rabbit
Isotype:
IgG
Antibody Type:
Polyclonal Antibody
Research Area:
Cell Biology

Description

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Antibody Name:Anti-SIGLEC8 Antibody
Antibody SKU:CAB10514
Antibody Size:20uL, 50uL, 100uL
Application:WB
Reactivity:Human, Mouse, Rat
Host Species:Rabbit
Immunogen:Recombinant fusion protein containing a sequence corresponding to amino acids 17-363 of human SIGLEC8 (NP_055257.2).
Application:WB
Recommended Dilution:WB 1:500 - 1:2000
Reactivity:Human, Mouse, Rat
Positive Samples:HL-60, 293T, Mouse liver, Rat blood
Immunogen:Recombinant fusion protein containing a sequence corresponding to amino acids 17-363 of human SIGLEC8 (NP_055257.2).
Purification Method:Affinity purification
Storage Buffer:Store at -20'C. Avoid freeze / thaw cycles. Buffer: PBS with 0.02% sodium azide, 50% glycerol, pH7.3.
Isotype:IgG
Sequence:MEGD RQYG DGYL LQVQ ELVT VQEG LCVH VPCS FSYP QDGW TDSD PVHG YWFR AGDR PYQD APVA TNNP DREV QAET QGRF QLLG DIWS NDCS LSIR DARK RDKG SYFF RLER GSMK WSYK SQLN YKTK QLSV FVTA LTHR PDIL ILGT LESG HSRN LTCS VPWA CKQG TPPM ISWI GASV SSPG PTTA RSSV LTLT PKPQ DHGT SLTC QVTL PGTG VTTT STVR LDVS YPPW NLTM TVFQ GDAT ASTA LGNG SSLS VLEG QSLR LVCA VNSN PPAR LSWT RGSL TLCP SRSS NPGL LELP RVHV RDEG EFTC RAQN AQGS QHIS LSLS LQNE GTGT SRPV SQVT LAA
Gene ID:27181
Uniprot:Q9NYZ4
Cellular Location:Membrane, Single-pass type I membrane protein
Calculated MW:44kDa/46kDa/54kDa
Observed MW:60kDa
Synonyms:SIGLEC8, SAF2, SIGLEC-8, SIGLEC8L
Background:
UniProt Protein Function:SIGLEC8: Putative adhesion molecule that mediates sialic-acid dependent binding to cells. Preferentially binds to alpha-2,3- linked sialic acid. Also binds to alpha-2,6-linked sialic acid. The sialic acid recognition site may be masked by cis interactions with sialic acids on the same cell surface. Belongs to the immunoglobulin superfamily. SIGLEC (sialic acid binding Ig-like lectin) family. 3 isoforms of the human protein are produced by alternative splicing.
UniProt Protein Details:

Protein type:Cell adhesion; Membrane protein, integral; Receptor, misc.

Chromosomal Location of Human Ortholog: 19q13.41

Molecular Function:protein binding; transmembrane receptor activity

Biological Process: signal transduction

NCBI Summary:Sialic acid-binding immunoglobulin (Ig)-like lectins, or SIGLECs (e.g., CD33 (MIM 159590)), are a family of type 1 transmembrane proteins each having a unique expression pattern, mostly in hemopoietic cells. SIGLEC8 is a member of the CD33-like subgroup of SIGLECs, which are localized to 19q13.3-q13.4 and have 2 conserved cytoplasmic tyrosine-based motifs: an immunoreceptor tyrosine-based inhibitory motif, or ITIM (see MIM 604964), and a motif homologous to one identified in signaling lymphocyte activation molecule (SLAM; MIM 603492) that mediates an association with SLAM-associated protein (SAP; MIM 300490) (summarized by Foussias et al., 2000 [PubMed 11095983]).[supplied by OMIM, May 2010]
UniProt Code:Q9NYZ4
NCBI GenInfo Identifier:25009268
NCBI Gene ID:27181
NCBI Accession:Q9NYZ4.2
UniProt Secondary Accession:Q9NYZ4,Q7Z728,
UniProt Related Accession:Q9NYZ4
Molecular Weight:54kDa
NCBI Full Name:Sialic acid-binding Ig-like lectin 8
NCBI Synonym Full Names:sialic acid binding Ig like lectin 8
NCBI Official Symbol:SIGLEC8  
NCBI Official Synonym Symbols:SAF2; SIGLEC-8; SIGLEC8L  
NCBI Protein Information:sialic acid-binding Ig-like lectin 8
UniProt Protein Name:Sialic acid-binding Ig-like lectin 8
UniProt Synonym Protein Names:Sialoadhesin family member 2; SAF-2
Protein Family:Sialic acid-binding Ig-like lectin
UniProt Gene Name:SIGLEC8  
Anti-SIGLEC8 Antibody (CAB10514)Western blot analysis of extracts of various cell lines, using SIGLEC8 antibody (CAB10514) at 1:1000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (CABS014) at 1:10000 dilution. Lysates/proteins: 25ug per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit (RM00020). Exposure time: 90s.
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