Cell Biology Antibodies 9

Anti-SIGLEC5 Antibody (CAB4250)

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SKU:
CAB4250
Product Type:
Antibody
Reactivity:
Human
Host Species:
Rabbit
Isotype:
IgG
Research Area:
Cell Biology

Description

system_update_altDatasheetsystem_update_altMSDS
Antibody Name:Anti-SIGLEC5 Antibody
Antibody SKU:CAB4250
Antibody Size:20uL, 50uL, 100uL
Application:WB
Reactivity:Human
Host Species:Rabbit
Immunogen:Recombinant fusion protein containing a sequence corresponding to amino acids 452-551 of human SIGLEC5 (NP_003821.1).
Application:WB
Recommended Dilution:WB 1:500 - 1:2000
Reactivity:Human
Positive Samples:K-562
Immunogen:Recombinant fusion protein containing a sequence corresponding to amino acids 452-551 of human SIGLEC5 (NP_003821.1).
Purification Method:Affinity purification
Storage Buffer:Store at -20'C. Avoid freeze / thaw cycles. Buffer: PBS with 0.02% sodium azide, 50% glycerol, pH7.3.
Isotype:IgG
Sequence:ICLC LIFF LIVK ARRK QAAG RPEK MDDE DPIM GTIT SGSR KKPW PDSP GDQA SPPG DAPP LEEQ KELH YASL SFSE MKSR EPKD QEAP STTE YSEI KTSK
Gene ID:8778
Uniprot:O15389
Cellular Location:Membrane, Single-pass type I membrane protein
Calculated MW:60kDa
Observed MW:140kDa
Synonyms:SIGLEC5, CD170, CD33L2, OB-BP2, OBBP2, SIGLEC-5
Background:This gene encodes a member of the sialic acid-binding immunoglobulin-like lectin (Siglec) family. These cell surface lectins are characterized by structural motifs in the immunoglobulin (Ig)-like domains and sialic acid recognition sites in the first Ig V set domain. The encoded protein is a member of the CD33-related subset of Siglecs and inhibits the activation of several cell types including monocytes, macrophages and neutrophils. Binding of group B Streptococcus (GBS) to the encoded protein plays a role in GBS immune evasion.
UniProt Protein Function:Putative adhesion molecule that mediates sialic-acid dependent binding to cells. Binds equally to alpha-2,3-linked and alpha-2,6-linked sialic acid. The sialic acid recognition site may be masked by cis interactions with sialic acids on the same cell surface.
NCBI Summary:This gene encodes a member of the sialic acid-binding immunoglobulin-like lectin (Siglec) family. These cell surface lectins are characterized by structural motifs in the immunoglobulin (Ig)-like domains and sialic acid recognition sites in the first Ig V set domain. The encoded protein is a member of the CD33-related subset of Siglecs and inhibits the activation of several cell types including monocytes, macrophages and neutrophils. Binding of group B Streptococcus (GBS) to the encoded protein plays a role in GBS immune evasion. [provided by RefSeq, Feb 2012]
UniProt Code:O15389
NCBI GenInfo Identifier:25009254
NCBI Gene ID:8778
NCBI Accession:O15389.1
UniProt Related Accession:O15389
Molecular Weight:60,715 Da
NCBI Full Name:Sialic acid-binding Ig-like lectin 5
NCBI Synonym Full Names:sialic acid binding Ig like lectin 5
NCBI Official Symbol:SIGLEC5  
NCBI Official Synonym Symbols:CD170; OBBP2; CD33L2; OB-BP2; SIGLEC-5  
NCBI Protein Information:sialic acid-binding Ig-like lectin 5
UniProt Protein Name:Sialic acid-binding Ig-like lectin 5
UniProt Synonym Protein Names:CD33 antigen-like 2; Obesity-binding protein 2; OB-BP2; OB-binding protein 2; CD_antigen: CD170
UniProt Gene Name:SIGLEC5  
Anti-SIGLEC5 Antibody (CAB4250)Western blot analysis of extracts of K-562 cells, using SIGLEC5 antibody (CAB4250) at 1:1000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (CABS014) at 1:10000 dilution. Lysates/proteins: 25ug per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit (RM00020). Exposure time: 1s.
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system_update_altDatasheetHuman SIGLEC5 Recombinant Protein SIGLEC5 contains 2 Ig-like C2-type (immunoglobulin-like) domains and 1 Ig-like V-type (immunoglobulin-like) domain. It belongs to the i