Cell Biology Antibodies 8
Anti-SIGLEC3/CD33 Antibody (CAB2059)
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- SKU:
- CAB2059
- Product Type:
- Antibody
- Reactivity:
- Human
- Reactivity:
- Mouse
- Reactivity:
- Rat
- Host Species:
- Rabbit
- Isotype:
- IgG
- Research Area:
- Cell Biology
Description
| Antibody Name: | Anti-SIGLEC3/CD33 Antibody |
| Antibody SKU: | CAB2059 |
| Antibody Size: | 20uL, 50uL, 100uL |
| Application: | WB IHC IF |
| Reactivity: | Human, Mouse, Rat |
| Host Species: | Rabbit |
| Immunogen: | Recombinant fusion protein containing a sequence corresponding to amino acids 49-259 of human SIGLEC3/CD33 (NP_001763.3). |
| Application: | WB IHC IF |
| Recommended Dilution: | WB 1:500 - 1:2000 IHC 1:50 - 1:200 IF 1:50 - 1:200 |
| Reactivity: | Human, Mouse, Rat |
| Positive Samples: | HeLa, K-562, HepG2 |
| Immunogen: | Recombinant fusion protein containing a sequence corresponding to amino acids 49-259 of human SIGLEC3/CD33 (NP_001763.3). |
| Purification Method: | Affinity purification |
| Storage Buffer: | Store at -20'C. Avoid freeze / thaw cycles. Buffer: PBS with 0.02% sodium azide, 50% glycerol, pH7.3. |
| Isotype: | IgG |
| Sequence: | YYDK NSPV HGYW FREG AIIS RDSP VATN KLDQ EVQE ETQG RFRL LGDP SRNN CSLS IVDA RRRD NGSY FFRM ERGS TKYS YKSP QLSV HVTD LTHR PKIL IPGT LEPG HSKN LTCS VSWA CEQG TPPI FSWL SAAP TSLG PRTT HSSV LIIT PRPQ DHGT NLTC QVKF AGAG VTTE RTIQ LNVT YVPQ NPTT GIFP GDGS GKQE TRAG VVH |
| Gene ID: | 945 |
| Uniprot: | P20138 |
| Cellular Location: | Cell membrane, Single-pass type I membrane protein |
| Calculated MW: | 25kDa/33kDa/39kDa |
| Observed MW: | 70kDa |
| Synonyms: | CD33, SIGLEC-3, SIGLEC3, p67 |
| Background: |
| UniProt Protein Function: | CD33: Putative adhesion molecule of myelomonocytic-derived cells that mediates sialic-acid dependent binding to cells. Preferentially binds to alpha-2,6-linked sialic acid. The sialic acid recognition site may be masked by cis interactions with sialic acids on the same cell surface. In the immune response, may act as an inhibitory receptor upon ligand induced tyrosine phosphorylation by recruiting cytoplasmic phosphatase(s) via their SH2 domain(s) that block signal transduction through dephosphorylation of signaling molecules. Induces apoptosis in acute myeloid leukemia (in vitro). Belongs to the immunoglobulin superfamily. SIGLEC (sialic acid binding Ig-like lectin) family. |
| UniProt Protein Details: | Protein type:Membrane protein, integral; Cell adhesion Chromosomal Location of Human Ortholog: 19q13.3 Cellular Component: integral to plasma membrane; plasma membrane; external side of plasma membrane Molecular Function:protein binding; receptor activity; carbohydrate binding Biological Process: negative regulation of cell proliferation; cell-cell signaling; signal transduction; cell adhesion |
| UniProt Code: | P20138 |
| NCBI GenInfo Identifier: | 116241290 |
| NCBI Gene ID: | 945 |
| NCBI Accession: | P20138.2 |
| UniProt Secondary Accession: | P20138,Q8TD24, B4E3P8, C9JEN7, F8WAL2, |
| UniProt Related Accession: | P20138 |
| Molecular Weight: | Calculated MW: 25kDa/33kDa/39kDaObserved MW: 77kDa |
| NCBI Full Name: | Myeloid cell surface antigen CD33 |
| NCBI Synonym Full Names: | CD33 molecule |
| NCBI Official Symbol: | CD33 |
| NCBI Official Synonym Symbols: | p67; SIGLEC3; SIGLEC-3 |
| NCBI Protein Information: | myeloid cell surface antigen CD33; gp67; CD33 antigen (gp67); sialic acid binding Ig-like lectin 3; sialic acid-binding Ig-like lectin 3 |
| UniProt Protein Name: | Myeloid cell surface antigen CD33 |
| UniProt Synonym Protein Names: | Sialic acid-binding Ig-like lectin 3; Siglec-3; gp67; CD_antigen: CD33 |
| Protein Family: | Myeloid cell surface antigen |
| UniProt Gene Name: | CD33 |
| UniProt Entry Name: | CD33_HUMAN |
 | Western blot analysis of extracts of various cell lines, using SIGLEC3/CD33 antibody (CAB2059) at 1:1000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (CABS014) at 1:10000 dilution. Lysates/proteins: 25ug per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit (RM00020). Exposure time: 5s. |
 | Immunohistochemistry of paraffin-embedded mouse spleen using SIGLEC3/CD33 antibody (CAB2059) at dilution of 1:200 (40x lens). |
 | Immunofluorescence analysis of THP-1 cells using SIGLEC3/CD33 antibody (CAB2059) at dilution of 1:100. Blue: DAPI for nuclear staining. |
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