Cell Biology Antibodies 12

Anti-SIGLEC12 Antibody (CAB8519)

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SKU:
CAB8519
Product Type:
Antibody
Applications:
WB
Reactivity:
Mouse
Host Species:
Rabbit
Isotype:
IgG
Research Area:
Cell Biology

Description

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Antibody Name:Anti-SIGLEC12 Antibody
Antibody SKU:CAB8519
Antibody Size:20uL, 50uL, 100uL
Application:WB
Reactivity:Mouse
Host Species:Rabbit
Immunogen:Recombinant fusion protein containing a sequence corresponding to amino acids 20-210 of human SIGLEC12 (NP_443729.1).
Application:WB
Recommended Dilution:WB 1:500 - 1:2000
Reactivity:Mouse
Positive Samples:Mouse spleen, Mouse heart
Immunogen:Recombinant fusion protein containing a sequence corresponding to amino acids 20-210 of human SIGLEC12 (NP_443729.1).
Purification Method:Affinity purification
Storage Buffer:Store at -20'C. Avoid freeze / thaw cycles. Buffer: PBS with 0.02% sodium azide, 50% glycerol, pH7.3.
Isotype:IgG
Sequence:EQKD YLLT MQKS VTVQ EGLC VSVL CSFS YPQN GWTA SDPV HGYW FRAG DHVS RNIP VATN NPAR AVQE ETRD RFHL LGDP QNKD CTLS IRDT RESD AGTY VFCV ERGN MKWN YKYD QLSV NVTA SQDL LSRY RLEV PESV TVQE GLCV SVPC SVLY PHYN WTAS SPVY GSWF KEGA DIPW DIPV ATNT PSG
Gene ID:89858
Uniprot:Q96PQ1
Cellular Location:Membrane, Single-pass type I membrane protein
Calculated MW:51kDa/64kDa
Observed MW:80kDa
Synonyms:SIGLEC12, S2V, SIGLECL1, SLG, Siglec-XII
Background:Sialic acid-binding immunoglobulin-like lectins (SIGLECs) are a family of cell surface proteins belonging to the immunoglobulin superfamily. They mediate protein-carbohydrate interactions by selectively binding to different sialic acid moieties present on glycolipids and glycoproteins. This gene encodes a member of the SIGLEC3-like subfamily of SIGLECs. Members of this subfamily are characterized by an extracellular V-set immunoglobulin-like domain followed by two C2-set immunoglobulin-like domains, and the cytoplasmic tyrosine-based motifs ITIM and SLAM-like. The encoded protein, upon tyrosine phosphorylation, has been shown to recruit the Src homology 2 domain-containing protein-tyrosine phosphatases SHP1 and SHP2. It has been suggested that the protein is involved in the negative regulation of macrophage signaling by functioning as an inhibitory receptor. This gene is located in a cluster with other SIGLEC3-like genes on 19q13.4. Alternative splicing results in multiple transcript variants.
UniProt Protein Function:SIGLEC12: Putative adhesion molecule that mediates sialic-acid dependent binding to cells. The sialic acid recognition site may be masked by cis interactions with sialic acids on the same cell surface. Belongs to the immunoglobulin superfamily. SIGLEC (sialic acid binding Ig-like lectin) family. 2 isoforms of the human protein are produced by alternative splicing.
UniProt Protein Details:

Protein type:Membrane protein, integral; Cell surface

Chromosomal Location of Human Ortholog: 19q13.4

NCBI Summary:Sialic acid-binding immunoglobulin-like lectins (SIGLECs) are a family of cell surface proteins belonging to the immunoglobulin superfamily. They mediate protein-carbohydrate interactions by selectively binding to different sialic acid moieties present on glycolipids and glycoproteins. This gene encodes a member of the SIGLEC3-like subfamily of SIGLECs. Members of this subfamily are characterized by an extracellular V-set immunoglobulin-like domain followed by two C2-set immunoglobulin-like domains, and the cytoplasmic tyrosine-based motifs ITIM and SLAM-like. The encoded protein, upon tyrosine phosphorylation, has been shown to recruit the Src homology 2 domain-containing protein-tyrosine phosphatases SHP1 and SHP2. It has been suggested that the protein is involved in the negative regulation of macrophage signaling by functioning as an inhibitory receptor. This gene is located in a cluster with other SIGLEC3-like genes on 19q13.4. Alternative splicing results in multiple transcript variants. [provided by RefSeq, Aug 2013]
UniProt Code:Q96PQ1
NCBI GenInfo Identifier:25009264
NCBI Gene ID:89858
NCBI Accession:Q96PQ1.1
UniProt Secondary Accession:Q96PQ1,Q8IYH7,
UniProt Related Accession:Q96PQ1
Molecular Weight:51,663 Da
NCBI Full Name:Sialic acid-binding Ig-like lectin 12
NCBI Synonym Full Names:sialic acid binding Ig like lectin 12 (gene/pseudogene)
NCBI Official Symbol:SIGLEC12  
NCBI Official Synonym Symbols:S2V; SLG; SIGLECL1; Siglec-XII  
NCBI Protein Information:sialic acid-binding Ig-like lectin 12
UniProt Protein Name:Sialic acid-binding Ig-like lectin 12
UniProt Synonym Protein Names:Sialic acid-binding Ig-like lectin-like 1; Siglec-L1
Protein Family:Sialic acid-binding Ig-like lectin
UniProt Gene Name:SIGLEC12  
UniProt Entry Name:SIG12_HUMAN
Anti-SIGLEC12 Polyclonal Antibody (CAB8519)Western blot analysis of extracts of various cell lines, using SIGLEC12 antibody (CAB8519) at 1:1000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (CABS014) at 1:10000 dilution. Lysates/proteins: 25ug per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit (RM00020). Exposure time: 90s.
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