Anti-Siglec-15 Antibody (CAB20773)
- SKU:
- CAB20773
Description
| Antibody Name: | Anti-Siglec-15 Antibody (CAB20773) |
| Antibody SKU: | CAB20773 |
| Antibody Size: | 50µL, 100µL |
| Application: | Western blotting, Immunohistochemistry |
| Reactivity: | Human, Mouse, Rat |
| Host Species: | Rabbit |
| Immunogen: | Recombinant fusion protein containing a sequence corresponding to amino acids 20-263 of human Siglec-15 (NP_998767.1). |
| Application: | Western blotting, Immunohistochemistry |
| Recommended Dilution: | WB 1:500 - 1:2000 IHC 1:50 - 1:200 |
| Reactivity: | Human, Mouse, Rat |
| Positive Samples: | K-562 |
| Immunogen: | Recombinant fusion protein containing a sequence corresponding to amino acids 20-263 of human Siglec-15 (NP_998767.1). |
| Purification Method: | Affinity purification |
| Storage Buffer: | Store at -20°C. Avoid freeze / thaw cycles. Buffer: PBS with 0.02% sodium azide, 50% glycerol, pH7.3. |
| Isotype: | IgG |
| Sequence: | FVRT KIDT TENL LNTE VHSS PAQR WSMQ VPPE VSAE AGDA AVLP CTFT HPHR HYDG PLTA IWRA GEPY AGPQ VFRC AAAR GSEL CQTA LSLH GRFR LLGN PRRN DLSL RVER LALA DDRR YFCR VEFA GDVH DRYE SRHG VRLH VTAA PRIV NISV LPSP AHAF RALC TAEG EPPP ALAW SGPA LGNS LAAV RSPR EGHG HLVT AELP ALTH DGRY TCTA ANSL GRSE ASVY LFRF HGAS GAST |
| Cellular Location: | |
| Calculated MW: | 35kDa |
| Observed MW: | 36KDa |
| Synonyms: | CD33L3, HsT1361, SIGLEC-15 |
| Background: | SIGLEC15 (Sialic Acid Binding Ig Like Lectin 15) is a Protein Coding gene. Diseases associated with SIGLEC15 include Osteoporosis, Juvenile and Osteoporosis. Among its related pathways are Innate Immune System and RET signaling. An important paralog of this gene is SIGLEC1. |
 | Immunohistochemistry of paraffin-embedded rat spleen using Siglec-15 Rabbit mAb at dilution of 1:100 (40x lens). Perform high pressure antigen retrieval with 10 mM citrate buffer pH 6. 0 before commencing with IHC staining protocol. |
 | Immunohistochemistry of paraffin-embedded mouse spleen using Siglec-15 Rabbit mAb at dilution of 1:100 (40x lens). Perform high pressure antigen retrieval with 10 mM citrate buffer pH 6. 0 before commencing with IHC staining protocol. |
 | Immunohistochemistry of paraffin-embedded rat brain using Siglec-15 Rabbit mAb at dilution of 1:100 (40x lens). Perform high pressure antigen retrieval with 10 mM citrate buffer pH 6. 0 before commencing with IHC staining protocol. |
 | Immunohistochemistry of paraffin-embedded human tonsil using Siglec-15 Rabbit mAb at dilution of 1:100 (40x lens). Perform high pressure antigen retrieval with 10 mM citrate buffer pH 6. 0 before commencing with IHC staining protocol. |
 | Immunohistochemistry of paraffin-embedded Human lung adenocarcinoma using Siglec-15 Rabbit mAb at dilution of 1:100 (40x lens). Perform high pressure antigen retrieval with 10 mM citrate buffer pH 6. 0 before commencing with IHC staining protocol. |
 | Immunohistochemistry of paraffin-embedded human colon using Siglec-15 Rabbit mAb at dilution of 1:100 (40x lens). Perform high pressure antigen retrieval with 10 mM citrate buffer pH 6. 0 before commencing with IHC staining protocol. |
 | Immunohistochemistry of paraffin-embedded human colon carcinoma using Siglec-15 Rabbit mAb at dilution of 1:100 (40x lens). Perform high pressure antigen retrieval with 10 mM citrate buffer pH 6. 0 before commencing with IHC staining protocol. |
 | Western blot analysis of extracts of K-562 cells, using Siglec-15 antibody at 1:1000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution. Lysates/proteins: 25ug per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Enhanced Kit. Exposure time: 180s. |
Additional Information
Product Type: |
Antibody |
Antibody Type: |
Secondary Antibody |
Host Species: |
Rabbit |
Isotype: |
Fluorescein conjugated IgG |
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