Cell Biology Antibodies 3
Anti-SIAE Antibody (CAB13217)
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- SKU:
- CAB13217
- Product Type:
- Antibody
- Reactivity:
- Human
- Host Species:
- Rabbit
- Isotype:
- IgG
- Antibody Type:
- Polyclonal Antibody
- Research Area:
- Cell Biology
Description
| Antibody Name: | Anti-SIAE Antibody |
| Antibody SKU: | CAB13217 |
| Antibody Size: | 20uL, 50uL, 100uL |
| Application: | WB |
| Reactivity: | Human |
| Host Species: | Rabbit |
| Immunogen: | Recombinant fusion protein containing a sequence corresponding to amino acids 270-450 of human SIAE (NP_733746.1). |
| Application: | WB |
| Recommended Dilution: | WB 1:500 - 1:2000 |
| Reactivity: | Human |
| Positive Samples: | 22Rv1, LO2 |
| Immunogen: | Recombinant fusion protein containing a sequence corresponding to amino acids 270-450 of human SIAE (NP_733746.1). |
| Purification Method: | Affinity purification |
| Storage Buffer: | Store at -20'C. Avoid freeze / thaw cycles. Buffer: PBS with 0.02% sodium azide, 50% glycerol, pH7.3. |
| Isotype: | IgG |
| Sequence: | LKGV VWYQ GESN INYN TDLY NCTF PALI EDWR ETFH RGSQ GQTE RFFP FGLV QLSS DLSK KSSD DGFP QIRW HQTA DFGY VPNP KMPN TFMA VAMD LCDR DSPF GSIH PRDK QTVA YRLH LGAR ALAY GEKN LTFE GPLP EKIE LLAH KGLL NLTY YQQI QVQK KDNK IFEI SCCS DHRC K |
| Gene ID: | 54414 |
| Uniprot: | Q9HAT2 |
| Cellular Location: | Lysosome, Secreted |
| Calculated MW: | 54kDa/58kDa |
| Observed MW: | 58kDa |
| Synonyms: | SIAE, AIS6, CSE-C, CSEC, LSE, YSG2 |
| Background: | This gene encodes an enzyme which removes 9-O-acetylation modifications from sialic acids. Mutations in this gene are associated with susceptibility to autoimmune disease 6. Multiple transcript variants encoding different isoforms, found either in the cytosol or in the lysosome, have been found for this gene. |
| UniProt Protein Function: | SIAE: Catalyzes the removal of O-acetyl ester groups from position 9 of the parent sialic acid, N-acetylneuraminic acid. Defects in SIAE are a cause of autoimmune disease type 6 (AIS6). Individuals manifesting susceptibility to autoimmune disease type 6 can suffer from juvenile idiopathic arthritis, rheumatoid arthritis, multiple sclerosis, Sjogren syndrome, systemic lupus erythematosus, type 1 diabetes, ulcerative colitis, and Crohn disease. 2 isoforms of the human protein are produced by alternative splicing. |
| UniProt Protein Details: | Protein type:Secreted, signal peptide; Hydrolase; EC 3.1.1.53; Secreted Chromosomal Location of Human Ortholog: 11q24 Cellular Component: extracellular space; lysosome Molecular Function:sialate O-acetylesterase activity Biological Process: regulation of immune system process; carbohydrate metabolic process Disease: Autoimmune Disease, Susceptibility To, 6 |
| NCBI Summary: | This gene encodes an enzyme which removes 9-O-acetylation modifications from sialic acids. Mutations in this gene are associated with susceptibility to autoimmune disease 6. Multiple transcript variants encoding different isoforms, found either in the cytosol or in the lysosome, have been found for this gene.[provided by RefSeq, Feb 2011] |
| UniProt Code: | Q9HAT2 |
| NCBI GenInfo Identifier: | 119587995 |
| NCBI Gene ID: | 54414 |
| NCBI Accession: | EAW67591.1 |
| UniProt Secondary Accession: | Q9HAT2,Q8IUT9, Q9HAU7, Q9NT71, B3KPB0, |
| UniProt Related Accession: | Q9HAT2 |
| Molecular Weight: | 58,315 Da |
| NCBI Full Name: | sialic acid acetylesterase |
| NCBI Synonym Full Names: | sialic acid acetylesterase |
| NCBI Official Symbol: | SIAE |
| NCBI Official Synonym Symbols: | LSE; AIS6; CSEC; YSG2; CSE-C |
| NCBI Protein Information: | sialate O-acetylesterase; H-Lse; sialic acid-specific acetylesterase II; cytosolic sialic acid 9-O-acetylesterase homolog |
| UniProt Protein Name: | Sialate O-acetylesterase |
| UniProt Synonym Protein Names: | H-Lse; Sialic acid-specific 9-O-acetylesterase |
| Protein Family: | Sialate O-acetylesterase |
| UniProt Gene Name: | SIAE |
| UniProt Entry Name: | SIAE_HUMAN |
 | Western blot analysis of extracts of various cell lines, using SIAE antibody (CAB13217) at 1:3000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (CABS014) at 1:10000 dilution. Lysates/proteins: 25ug per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit (RM00020). Exposure time: 1s. |
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