Name: Anti-SHPK Antibody (CAB8823)
Brand: Cell Biology Antibodies 12
SKU: CAB8823
Availability: OutOfStock

Antibody Name:	Anti-SHPK Antibody
Antibody SKU:	CAB8823
Antibody Size:	20uL, 50uL, 100uL
Application:	WB
Reactivity:	Human, Mouse, Rat
Host Species:	Rabbit
Immunogen:	Recombinant fusion protein containing a sequence corresponding to amino acids 1-320 of human SHPK (NP_037408.2).

Application:	WB
Recommended Dilution:	WB 1:500 - 1:2000
Reactivity:	Human, Mouse, Rat
Positive Samples:	A375, Mouse kidney, Rat testis

Immunogen:	Recombinant fusion protein containing a sequence corresponding to amino acids 1-320 of human SHPK (NP_037408.2).
Purification Method:	Affinity purification
Storage Buffer:	Store at -20'C. Avoid freeze / thaw cycles. Buffer: PBS with 0.02% sodium azide, 50% glycerol, pH7.3.
Isotype:	IgG
Sequence:	MAAR PITL GIDL GTTS VKAA LLRA APDD PSGF AVLA SCAR AARA EAAV ESAV AGPQ GREQ DVSR ILQA LHEC LAAL PRPQ LRSV VGIG VSGQ MHGV VFWK TGQG CEWT EGGI TPVF EPRA VSHL VTWQ DGRC SSEF LASL PQPK SHLS VATG FGCA TIFW LLKY RPEF LKSY DAAG TIHD YVVA MLCG LPRP LMSD QNAA SWGY FNTQ SQSW NVET LRSS GFPV HLLP DIAE PGSV AGRT SHMW FEIP KGTQ VGVA LGDL QASV YSCM AQRT DAVL NIST SVQL AASM PSGF QPAQ TPDP TAPV AYFP YFNR TYLG VAAS
Gene ID:	23729
Uniprot:	Q9UHJ6
Cellular Location:	Cytoplasm
Calculated MW:	51kDa
Observed MW:	50kDa

Synonyms:

SHPK, CARKL, SHK

Background:

The protein encoded by this gene has weak homology to several carbohydrate kinases, a class of proteins involved in the phosphorylation of sugars as they enter a cell, inhibiting return across the cell membrane. Sequence variation between this novel gene and known carbohydrate kinases suggests the possibility of a different substrate, cofactor or changes in kinetic properties distinguishing it from other carbohydrate kinases. The gene resides in a region commonly deleted in cystinosis patients, suggesting a role as a modifier for the cystinosis phenotype. The genomic region is also rich in Alu repetitive sequences, frequently involved in chromosomal rearrangements.

UniProt Protein Function:	SHPK: has weak homology to several carbohydrate kinases, a class of proteins involved in the phosphorylation of sugars as they enter a cell, inhibiting return across the cell membrane. Sequence variation between this novel gene and known carbohydrate kinases suggests the possibility of a different substrate, cofactor or changes in kinetic properties distinguishing it from other carbohydrate kinases. The gene resides in a region commonly deleted in cystinosis patients, suggesting a role as a modifier for the cystinosis phenotype. The genomic region is also rich in Alu repetitive sequences, frequently involved in chromosomal rearrangements. [provided by RefSeq, Jul 2008]
UniProt Protein Details:	Protein type:EC 2.7.1.14; Kinase, other Chromosomal Location of Human Ortholog: 17p13 Cellular Component: cytoplasm Molecular Function:sedoheptulokinase activity; ATP binding Biological Process: regulation of macrophage activation; carbohydrate metabolic process; regulation of inflammatory response; pentose-phosphate shunt, non-oxidative branch; phosphorylation
NCBI Summary:	The protein encoded by this gene has weak homology to several carbohydrate kinases, a class of proteins involved in the phosphorylation of sugars as they enter a cell, inhibiting return across the cell membrane. Sequence variation between this novel gene and known carbohydrate kinases suggests the possibility of a different substrate, cofactor or changes in kinetic properties distinguishing it from other carbohydrate kinases. The gene resides in a region commonly deleted in cystinosis patients, suggesting a role as a modifier for the cystinosis phenotype. The genomic region is also rich in Alu repetitive sequences, frequently involved in chromosomal rearrangements. [provided by RefSeq, Jul 2008]
UniProt Code:	Q9UHJ6
NCBI GenInfo Identifier:	74315356
NCBI Gene ID:	23729
NCBI Accession:	NP_037408.2
UniProt Secondary Accession:	Q9UHJ6,Q8WUH3, B2R640,
UniProt Related Accession:	Q9UHJ6
Molecular Weight:	51,491 Da
NCBI Full Name:	sedoheptulokinase
NCBI Synonym Full Names:	sedoheptulokinase
NCBI Official Symbol:	SHPK
NCBI Official Synonym Symbols:	SHK; CARKL
NCBI Protein Information:	sedoheptulokinase
UniProt Protein Name:	Sedoheptulokinase
UniProt Synonym Protein Names:	Carbohydrate kinase-like protein
Protein Family:	Sedoheptulokinase
UniProt Gene Name:	SHPK
UniProt Entry Name:	SHPK_HUMAN

Western blot analysis of extracts of various cell lines, using SHPK antibody (CAB8823) at 1:3000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (CABS014) at 1:10000 dilution. Lysates/proteins: 25ug per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit (RM00020). Exposure time: 90s.

Antibodies

SHPK Antibody (PACO02772)

SHPK Antibody (PACO12203)

Secondary Antibody

Anti-HRP Goat Anti-Rabbit IgG (H+L) Antibody (CABS014)