Metabolism Antibodies 1

Anti-SHMT1 Antibody (CAB12489)

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SKU:
CAB12489
Product Type:
Antibody
Reactivity:
Human
Reactivity:
Mouse
Reactivity:
Rat
Host Species:
Rabbit
Isotype:
IgG
Antibody Type:
Polyclonal Antibody
Research Area:
Metabolism

Description

system_update_altDatasheetsystem_update_altMSDS
Antibody Name:Anti-SHMT1 Antibody
Antibody SKU:CAB12489
Antibody Size:20uL, 50uL, 100uL
Application:WB IHC IF
Reactivity:Human, Mouse, Rat
Host Species:Rabbit
Immunogen:Recombinant fusion protein containing a sequence corresponding to amino acids 285-444 of human SHMT1 (NP_683718.1).
Application:WB IHC IF
Recommended Dilution:WB 1:500 - 1:2000 IHC 1:50 - 1:200 IF 1:50 - 1:200
Reactivity:Human, Mouse, Rat
Positive Samples:293T, HepG2, HT-29, K-562, A375, Mouse liver
Immunogen:Recombinant fusion protein containing a sequence corresponding to amino acids 285-444 of human SHMT1 (NP_683718.1).
Purification Method:Affinity purification
Storage Buffer:Store at -20'C. Avoid freeze / thaw cycles. Buffer: PBS with 0.02% sodium azide, 50% glycerol, pH7.3.
Isotype:IgG
Sequence:FKVY QHQV VANC RALS EALT ELGY KIVT GGSD NHLI LVDL RSKG TDGG RAEK VLEA CSIA CNKN TCPG DRSA LRPS GLRL GTPA LTSR GLLE KDFQ KVAH FIHR GIEL TLQI QSDT GVRA TLKE FKER LAGD KYQA AVQA LREE VESF ASLF PLPG LPDF
Gene ID:6470
Uniprot:P34896
Cellular Location:Cytoplasm
Calculated MW:37kDa/44kDa/49kDa/53kDa
Observed MW:53kDa
Synonyms:SHMT1, CSHMT, SHMT
Background:This gene encodes the cytosolic form of serine hydroxymethyltransferase, a pyridoxal phosphate-containing enzyme that catalyzes the reversible conversion of serine and tetrahydrofolate to glycine and 5, 10-methylene tetrahydrofolate. This reaction provides one-carbon units for synthesis of methionine, thymidylate, and purines in the cytoplasm. This gene is located within the Smith-Magenis syndrome region on chromosome 17. A pseudogene of this gene is located on the short arm of chromosome 1. Alternative splicing results in multiple transcript variants.
UniProt Protein Function:SHMT1: Interconversion of serine and glycine. Belongs to the SHMT family. 3 isoforms of the human protein are produced by alternative splicing.
UniProt Protein Details:

Protein type:Cofactor and Vitamin Metabolism - one carbon pool by folate; EC 2.1.2.1; Amino Acid Metabolism - glycine, serine and threonine; Energy Metabolism - methane; Other Amino Acids Metabolism - cyanoamino acid; Lyase; Methyltransferase; Mitochondrial

Chromosomal Location of Human Ortholog: 17p11.2

Cellular Component: nucleoplasm; mitochondrion; cytoplasm; cytosol; nucleus

Molecular Function:L-allo-threonine aldolase activity; amino acid binding; protein homodimerization activity; glycine hydroxymethyltransferase activity; pyridoxal phosphate binding

Biological Process: purine base biosynthetic process; vitamin metabolic process; folic acid metabolic process; L-serine catabolic process; glycine biosynthetic process from serine; water-soluble vitamin metabolic process; carnitine biosynthetic process; protein tetramerization; protein homotetramerization

NCBI Summary:This gene encodes the cytosolic form of serine hydroxymethyltransferase, a pyridoxal phosphate-containing enzyme that catalyzes the reversible conversion of serine and tetrahydrofolate to glycine and 5,10-methylene tetrahydrofolate. This reaction provides one-carbon units for synthesis of methionine, thymidylate, and purines in the cytoplasm. This gene is located within the Smith-Magenis syndrome region on chromosome 17. A pseudogene of this gene is located on the short arm of chromosome 1. Alternative splicing results in multiple transcript variants. [provided by RefSeq, Aug 2013]
UniProt Code:P34896
NCBI GenInfo Identifier:462184
NCBI Gene ID:6470
NCBI Accession:P34896.1
UniProt Related Accession:P34896
Molecular Weight:
NCBI Full Name:Serine hydroxymethyltransferase, cytosolic
NCBI Synonym Full Names:serine hydroxymethyltransferase 1
NCBI Official Symbol:SHMT1  
NCBI Official Synonym Symbols:SHMT; CSHMT  
NCBI Protein Information:serine hydroxymethyltransferase, cytosolic
UniProt Protein Name:Serine hydroxymethyltransferase, cytosolic
UniProt Synonym Protein Names:Glycine hydroxymethyltransferase; Serine methylase
Protein Family:Serine hydroxymethyltransferase
UniProt Gene Name:SHMT1  
UniProt Entry Name:GLYC_HUMAN
Anti-SHMT1 Antibody (CAB12489)Western blot analysis of extracts of various cell lines, using SHMT1 antibody (CAB12489) at 1:1000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (CABS014) at 1:10000 dilution. Lysates/proteins: 25ug per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit (RM00020). Exposure time: 10s.
Anti-SHMT1 Antibody (CAB12489)Immunohistochemistry of paraffin-embedded rat kidney using SHMT1 antibody (CAB12489) at dilution of 1:100 (40x lens).
Anti-SHMT1 Antibody (CAB12489)Immunohistochemistry of paraffin-embedded mouse kidney using SHMT1 antibody (CAB12489) at dilution of 1:100 (40x lens).
Anti-SHMT1 Antibody (CAB12489)Immunofluorescence analysis of U-2 OS cells using SHMT1 antibody (CAB12489) at dilution of 1:100. Blue: DAPI for nuclear staining.
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