Antibody Name:	Anti-SHBG Antibody
Antibody SKU:	CAB7450
Antibody Size:	20uL, 50uL, 100uL
Application:	WB IHC
Reactivity:	Human, Mouse, Rat
Host Species:	Rabbit
Immunogen:	Recombinant fusion protein containing a sequence corresponding to amino acids 30-170 of human SHBG (NP_001031.2).

Application:	WB IHC
Recommended Dilution:	WB 1:500 - 1:2000 IHC 1:100 - 1:200
Reactivity:	Human, Mouse, Rat
Positive Samples:	A-549, Human placenta, Mouse testis

Immunogen:	Recombinant fusion protein containing a sequence corresponding to amino acids 30-170 of human SHBG (NP_001031.2).
Purification Method:	Affinity purification
Storage Buffer:	Store at -20'C. Avoid freeze / thaw cycles. Buffer: PBS with 0.02% sodium azide, 50% glycerol, pH7.3.
Isotype:	IgG
Sequence:	LRPV LPTQ SAHD PPAV HLSN GPGQ EPIA VMTF DLTK ITKT SSSF EVRT WDPE GVIF YGDT NPKD DWFM LGLR DGRP EIQL HNHW AQLT VGAG PRLD DGRW HQVE VKME GDSV LLEV DGEE VLRL RQVS GPLT SKRH PIMR I
Gene ID:	6462
Uniprot:	P04278
Cellular Location:	Secreted
Calculated MW:	31kDa/32kDa/41kDa/43kDa
Observed MW:	44kDa

Synonyms:

SHBG, ABP, SBP, TEBG

Background:

This gene encodes a steroid binding protein that was first described as a plasma protein secreted by the liver but is now thought to participate in the regulation of steroid responses. The encoded protein transports androgens and estrogens in the blood, binding each steroid molecule as a dimer formed from identical or nearly identical monomers. Polymorphisms in this gene have been associated with polycystic ovary syndrome and type 2 diabetes mellitus. Alternative splicing results in multiple transcript variants.

UniProt Protein Function:	SHBG: Functions as an androgen transport protein, but may also be involved in receptor mediated processes. Each dimer binds one molecule of steroid. Specific for 5-alpha-dihydrotestosterone, testosterone, and 17-beta-estradiol. Regulates the plasma metabolic clearance rate of steroid hormones by controlling their plasma concentration. 2 isoforms of the human protein are produced by alternative splicing.
UniProt Protein Details:	Protein type:Secreted; Secreted, signal peptide Chromosomal Location of Human Ortholog: 17p13.1 Cellular Component: extracellular region Molecular Function:androgen binding Biological Process: primary spermatocyte growth
NCBI Summary:	This gene encodes a steroid binding protein that was first described as a plasma protein secreted by the liver but is now thought to participate in the regulation of steroid responses. The encoded protein binds each steroid molecule as a dimer formed from identical or nearly identical monomers. The use of alternate promoters and alternatively spliced transcripts have been described. Multiple transcript variants encoding different isoforms have been found for this gene. [provided by RefSeq, Apr 2009]
UniProt Code:	P04278
NCBI GenInfo Identifier:	7382460
NCBI Gene ID:	6462
NCBI Accession:	NP_001031.2
UniProt Secondary Accession:	P04278,P14689, Q16616, Q3MIL0, Q6ISD2, B0FWH4, E9PGW1 F5H5Z8, I3L1N7,
UniProt Related Accession:	P04278
Molecular Weight:	43,779 Da
NCBI Full Name:	sex hormone-binding globulin isoform 1
NCBI Synonym Full Names:	sex hormone-binding globulin
NCBI Official Symbol:	SHBG
NCBI Official Synonym Symbols:	ABP; SBP; TEBG
NCBI Protein Information:	sex hormone-binding globulin; sex steroid-binding protein; testosterone-binding beta-globulin; testosterone-estrogen-binding globulin; testosterone-estradiol-binding globulin; testis-specific androgen-binding protein
UniProt Protein Name:	Sex hormone-binding globulin
UniProt Synonym Protein Names:	Sex steroid-binding protein; SBP; Testis-specific androgen-binding protein; ABP; Testosterone-estradiol-binding globulin; TeBG; Testosterone-estrogen-binding globulin
Protein Family:	Sex hormone-binding globulin
UniProt Gene Name:	SHBG
UniProt Entry Name:	SHBG_HUMAN

	Western blot analysis of extracts of various cell lines, using SHBG antibody (CAB7450) at 1:1000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (CABS014) at 1:10000 dilution. Lysates/proteins: 25ug per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit (RM00020). Exposure time: 30s.
	Immunohistochemistry of paraffin-embedded human liver cancer using SHBG antibody (CAB7450) at dilution of 1:100 (40x lens).