Signal Transduction Antibodies 1

Anti-SHARPIN Antibody (CAB12240)

(No reviews yet) Write a Review
SKU:
CAB12240
Product Type:
Antibody
Reactivity:
Human
Reactivity:
Mouse
Reactivity:
Rat
Host Species:
Rabbit
Isotype:
IgG
Antibody Type:
Polyclonal Antibody
Research Area:
Signal Transduction

Description

system_update_altDatasheetsystem_update_altMSDS
Antibody Name:Anti-SHARPIN Antibody
Antibody SKU:CAB12240
Antibody Size:20uL, 50uL, 100uL
Application:WB IF
Reactivity:Human, Mouse, Rat
Host Species:Rabbit
Immunogen:Recombinant fusion protein containing a sequence corresponding to amino acids 1-170 of human SHARPIN (NP_112236.3).
Application:WB IF
Recommended Dilution:WB 1:500 - 1:2000 IF 1:50 - 1:100
Reactivity:Human, Mouse, Rat
Positive Samples:293T, LO2, HeLa, A-549, Rat brain
Immunogen:Recombinant fusion protein containing a sequence corresponding to amino acids 1-170 of human SHARPIN (NP_112236.3).
Purification Method:Affinity purification
Storage Buffer:Store at -20'C. Avoid freeze / thaw cycles. Buffer: PBS with 0.02% sodium azide, 50% glycerol, pH7.3.
Isotype:IgG
Sequence:MAPP AGGA AAAA SDLG SAAV LLAV HAAV RPLG AGPD AEAQ LRRL QLSA DPER PGRF RLEL LGAG PGAV NLEW PLES VSYT IRGP TQHE LQPP PGGP GTLS LHFL NPQE AQRW AVLV RGAT VEGQ NGSK SNSP PALG PEAC PVSL PSPP EAST LKGP PPEA DLPR SPGN LT
Gene ID:81858
Uniprot:Q9H0F6
Cellular Location:Cell junction, Cytoplasm, cytosol, synapse
Calculated MW:33kDa/39kDa
Observed MW:45kDa
Synonyms:SHARPIN, SIPL1, sharpin
Background:
UniProt Protein Function:Function: Component of the LUBAC complex which conjugates linear polyubiquitin chains in a head-to-tail manner to substrates and plays a key role in NF-kappa-B activation and regulation of inflammation. LUBAC conjugates linear polyubiquitin to IKBKG and RIPK1 and is involved in activation of the canonical NF-kappa-B and the JNK signaling pathways. Linear ubiquitination mediated by the LUBAC complex interferes with TNF-induced cell death and thereby prevents inflammation. LUBAC is proposed to be recruited to the TNF-R1 signaling complex (TNF-RSC) following polyubiquitination of TNF-RSC components by BIRC2 and/or BIRC3 and to conjugate linear polyubiquitin to IKBKG and possibly other components contributing to the stability of the complex. Together with FAM105B/otulin, the LUBAC complex regulates the canonical Wnt signaling during angiogenesis. Ref.10 Ref.11 Ref.12
UniProt Protein Details:

Subunit structure: Monomer and homodimer. Interacts with SHANK1, EYA1 and EYA2

By similarity. Component of the LUBAC complex (linear ubiquitin chain assembly complex) which consists of SHARPIN, RBCK1 and RNF31. LUBAC has a MW of approximative 600 kDa suggesting a heteromultimeric assembly of its subunits. Associates with the TNF-R1 signaling complex (TNF-RSC) in a stimulation-dependent manner. Ref.10 Ref.11 Ref.12 Ref.13

Subcellular location: Cytoplasm › cytosol Ref.2.

Tissue specificity: Highly expressed in skeletal muscle and placenta and at lower levels in brain, heart, colon without mucosa, thymus, spleen, kidney, liver, small intestine, lung and peripheral blood leukocytes. Up-regulated in various tumor tissues such as kidney, liver, ovary and pancreas tumors. Ref.2

Domain: The Ubiquitin-like domain is required for the interaction with RNF31. Ref.10 Ref.11 Ref.12The RanBP2-type zinc fingers mediate the specific interaction with ubiquitin. Binds preferentially linear polyubiquitin chains and 'Lys-63'-linked polyubiquitin chains over 'Lys-48'-linked polyubiquitin chains. Also binds monoubiquitin. Ref.10 Ref.11 Ref.12

Sequence similarities: Contains 1 RanBP2-type zinc finger.Contains 1 ubiquitin-like domain.

Sequence caution: The sequence BAC11666.1 differs from that shown. Reason: Frameshift at position 334. The sequence BAC53797.1 differs from that shown. Reason: Frameshift at position 218. The sequence BAC53798.1 differs from that shown. Reason: Aberrant splicing.

UniProt Code:Q9H0F6
NCBI GenInfo Identifier:118918415
NCBI Gene ID:81858
NCBI Accession:NP_112236.3
UniProt Secondary Accession:Q9H0F6,Q8IXF5, Q8IXF6, Q8N2E7, Q8TB25, Q9BUE4, A6NEG3 C0L3L2, D3DWL3,
UniProt Related Accession:Q9H0F6
Molecular Weight:39,949 Da
NCBI Full Name:sharpin
NCBI Synonym Full Names:SHANK-associated RH domain interactor
NCBI Official Symbol:SHARPIN  
NCBI Official Synonym Symbols:SIPL1  
NCBI Protein Information:sharpin; hSIPL1; shank-interacting protein-like 1; shank-associated RH domain-interacting protein
UniProt Protein Name:Sharpin
UniProt Synonym Protein Names:Shank-associated RH domain-interacting protein; Shank-interacting protein-like 1
Protein Family:Sharpin
UniProt Gene Name:SHARPIN  
UniProt Entry Name:SHRPN_HUMAN
Anti-SHARPIN Antibody (CAB12240)Western blot analysis of extracts of various cell lines, using SHARPIN antibody (CAB12240) at 1:3000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (CABS014) at 1:10000 dilution. Lysates/proteins: 25ug per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit (RM00020). Exposure time: 3s.
View AllClose

0 Reviews

View AllClose

Related Products

Human SHARPIN (Sharpin) ELISA Kit (HUFI06539)

Human SHARPIN (Sharpin) ELISA Kit (HUFI06539)

Human SHARPIN (Sharpin) ELISASHARPIN (Sharpin) is a transcriptional repressor which acts as a negative regulator of Hedgehog (Hh) gene expression. SHARPIN (Sharpin) has been shown to repress...