Signal Transduction Antibodies 1
Anti-SHARPIN Antibody (CAB11714)
- SKU:
- CAB11714
- Product Type:
- Antibody
- Reactivity:
- Human
- Reactivity:
- Rat
- Host Species:
- Rabbit
- Isotype:
- IgG
- Antibody Type:
- Polyclonal Antibody
- Research Area:
- Signal Transduction
Description
| Antibody Name: | Anti-SHARPIN Antibody |
| Antibody SKU: | CAB11714 |
| Antibody Size: | 20uL, 50uL, 100uL |
| Application: | WB |
| Reactivity: | Human, Rat |
| Host Species: | Rabbit |
| Immunogen: | Recombinant fusion protein containing a sequence corresponding to amino acids 1-170 of human SHARPIN (NP_112236.3). |
| Application: | WB |
| Recommended Dilution: | WB 1:500 - 1:2000 |
| Reactivity: | Human, Rat |
| Positive Samples: | 293T, LO2, HeLa, A-549, Rat brain |
| Immunogen: | Recombinant fusion protein containing a sequence corresponding to amino acids 1-170 of human SHARPIN (NP_112236.3). |
| Purification Method: | Affinity purification |
| Storage Buffer: | Store at -20'C. Avoid freeze / thaw cycles. Buffer: PBS with 0.02% sodium azide, 50% glycerol, pH7.3. |
| Isotype: | IgG |
| Sequence: | MAPP AGGA AAAA SDLG SAAV LLAV HAAV RPLG AGPD AEAQ LRRL QLSA DPER PGRF RLEL LGAG PGAV NLEW PLES VSYT IRGP TQHE LQPP PGGP GTLS LHFL NPQE AQRW AVLV RGAT VEGQ NGSK SNSP PALG PEAC PVSL PSPP EAST LKGP PPEA DLPR SPGN LT |
| Gene ID: | 81858 |
| Uniprot: | Q9H0F6 |
| Cellular Location: | Cell junction, Cytoplasm, cytosol, synapse |
| Calculated MW: | 33kDa/39kDa |
| Observed MW: | 40kDa |
| Synonyms: | SHARPIN, SIPL1, sharpin |
| Background: |
| UniProt Protein Function: | Function: Component of the LUBAC complex which conjugates linear polyubiquitin chains in a head-to-tail manner to substrates and plays a key role in NF-kappa-B activation and regulation of inflammation. LUBAC conjugates linear polyubiquitin to IKBKG and RIPK1 and is involved in activation of the canonical NF-kappa-B and the JNK signaling pathways. Linear ubiquitination mediated by the LUBAC complex interferes with TNF-induced cell death and thereby prevents inflammation. LUBAC is proposed to be recruited to the TNF-R1 signaling complex (TNF-RSC) following polyubiquitination of TNF-RSC components by BIRC2 and/or BIRC3 and to conjugate linear polyubiquitin to IKBKG and possibly other components contributing to the stability of the complex. Together with FAM105B/otulin, the LUBAC complex regulates the canonical Wnt signaling during angiogenesis. Ref.10 Ref.11 Ref.12 |
| UniProt Protein Details: | Subunit structure: Monomer and homodimer. Interacts with SHANK1, EYA1 and EYA2 By similarity. Component of the LUBAC complex (linear ubiquitin chain assembly complex) which consists of SHARPIN, RBCK1 and RNF31. LUBAC has a MW of approximative 600 kDa suggesting a heteromultimeric assembly of its subunits. Associates with the TNF-R1 signaling complex (TNF-RSC) in a stimulation-dependent manner. Ref.10 Ref.11 Ref.12 Ref.13 Subcellular location: Cytoplasm cytosol Ref.2. Tissue specificity: Highly expressed in skeletal muscle and placenta and at lower levels in brain, heart, colon without mucosa, thymus, spleen, kidney, liver, small intestine, lung and peripheral blood leukocytes. Up-regulated in various tumor tissues such as kidney, liver, ovary and pancreas tumors. Ref.2 Domain: The Ubiquitin-like domain is required for the interaction with RNF31. Ref.10 Ref.11 Ref.12The RanBP2-type zinc fingers mediate the specific interaction with ubiquitin. Binds preferentially linear polyubiquitin chains and 'Lys-63'-linked polyubiquitin chains over 'Lys-48'-linked polyubiquitin chains. Also binds monoubiquitin. Ref.10 Ref.11 Ref.12 Sequence similarities: Contains 1 RanBP2-type zinc finger.Contains 1 ubiquitin-like domain. Sequence caution: The sequence BAC11666.1 differs from that shown. Reason: Frameshift at position 334. The sequence BAC53797.1 differs from that shown. Reason: Frameshift at position 218. The sequence BAC53798.1 differs from that shown. Reason: Aberrant splicing. |
| UniProt Code: | Q9H0F6 |
| NCBI GenInfo Identifier: | 118918415 |
| NCBI Gene ID: | 81858 |
| NCBI Accession: | NP_112236.3 |
| UniProt Secondary Accession: | Q9H0F6,Q8IXF5, Q8IXF6, Q8N2E7, Q8TB25, Q9BUE4, A6NEG3 C0L3L2, D3DWL3, |
| UniProt Related Accession: | Q9H0F6 |
| Molecular Weight: | 39,949 Da |
| NCBI Full Name: | sharpin |
| NCBI Synonym Full Names: | SHANK-associated RH domain interactor |
| NCBI Official Symbol: | SHARPIN |
| NCBI Official Synonym Symbols: | SIPL1 |
| NCBI Protein Information: | sharpin; hSIPL1; shank-interacting protein-like 1; shank-associated RH domain-interacting protein |
| UniProt Protein Name: | Sharpin |
| UniProt Synonym Protein Names: | Shank-associated RH domain-interacting protein; Shank-interacting protein-like 1 |
| Protein Family: | Sharpin |
| UniProt Gene Name: | SHARPIN |
| UniProt Entry Name: | SHRPN_HUMAN |
 | Western blot analysis of extracts of various cell lines, using SHARPIN Antibody (CAB11714) at 1:3000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (CABS014) at 1:10000 dilution. Lysates/proteins: 25ug per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit (RM00020). Exposure time: 10s. |
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