Cell Death Antibodies 1
Anti-SH3KBP1 Antibody (CAB1952)
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- SKU:
- CAB1952
- Product Type:
- Antibody
- Reactivity:
- Human
- Reactivity:
- Mouse
- Host Species:
- Rabbit
- Isotype:
- IgG
- Research Area:
- Cell Death
Description
| Antibody Name: | Anti-SH3KBP1 Antibody |
| Antibody SKU: | CAB1952 |
| Antibody Size: | 20uL, 50uL, 100uL |
| Application: | WB |
| Reactivity: | Human, Mouse |
| Host Species: | Rabbit |
| Immunogen: | Recombinant fusion protein containing a sequence corresponding to amino acids 136-271 of human SH3KBP1 (NP_114098.1). |
| Application: | WB |
| Recommended Dilution: | WB 1:500 - 1:2000 |
| Reactivity: | Human, Mouse |
| Positive Samples: | THP-1 |
| Immunogen: | Recombinant fusion protein containing a sequence corresponding to amino acids 136-271 of human SH3KBP1 (NP_114098.1). |
| Purification Method: | Affinity purification |
| Storage Buffer: | Store at -20'C. Avoid freeze / thaw cycles. Buffer: PBS with 0.02% sodium azide, 50% glycerol, pH7.3. |
| Isotype: | IgG |
| Sequence: | WEGV LNGK TGMF PSNF IKEL SGES DELG ISQD EQLS KSSL RETT GSES DGGD SSST KSEG ANGT VATA AIQP KKVK GVGF GDIF KDKP IKLR PRSI EVEN DFLP VEKT IGKK LPAT TATP DSSK TEMD SRTK SKDY |
| Gene ID: | 30011 |
| Uniprot: | Q96B97 |
| Cellular Location: | Cell junction, Cytoplasm, Cytoplasmic vesicle membrane, Peripheral membrane protein, cytoskeleton, focal adhesion, synapse, synaptosome |
| Calculated MW: | 46kDa/68kDa/73kDa |
| Observed MW: | 73kDa |
| Synonyms: | SH3KBP1, CD2BP3, CIN85, GIG10, HSB-1, HSB1, MIG18 |
| Background: | This gene encodes an adapter protein that contains three N-terminal Src homology domains, a proline rich region and a C-terminal coiled-coil domain. The encoded protein facilitates protein-protein interactions and has been implicated in numerous cellular processes including apoptosis, cytoskeletal rearrangement, cell adhesion and in the regulation of clathrin-dependent endocytosis. Alternate splicing results in multiple transcript variants. |
| UniProt Protein Function: | SH3KBP1: Adapter protein involved in regulating diverse signal transduction pathways. Involved in the regulation of endocytosis and lysosomal degradation of ligand-induced receptor tyrosine kinases, including EGFR and MET/hepatocyte growth factor receptor, through a association with CBL and endophilins. The association with CBL, and thus the receptor internalization, may inhibited by an interaction with PDCD6IP and/or SPRY2. Involved in regulation of ligand-dependent endocytosis of the IgE receptor. Attenuates phosphatidylinositol 3-kinase activity by interaction with its regulatory subunit. May be involved in regulation of cell adhesion; promotes the interaction between TTK2B and PDCD6IP. May be involved in the regulation of cellular stress response via the MAPK pathways through its interaction with MAP3K4. Is involved in modulation of tumor necrosis factor mediated apoptosis. Can self-associate and form homotetramers. Interacts with CD2, F-actin capping protein, PIK3R3, GRB2, EGFR, MET, BLNK, MAP3K4, PDCD6IP, SPRY2, ARHGAP17, ARHGAP27, MAGI2, CRK, BCAR1, SOS1, ASAP1, ARAP3, HIP1R, SYNJ2, INPP5D and STAP1. Interacts with CBL and CBLB, but does not interact with CBLC. Two molecules of SH3KBP1 seem to bind through their respective SH3 1 domain to one molecule of CBLB. The interaction with CBL or CBLB and EGFR is increased upon EGF stimulation. The interaction with CBL is attenuated by PDCD6IP. Interacts through its proline-rich region with the SH3 domain of endophilins SH3GL1, SH3GL2 and SH3GL3. The SH3KBP1-endophilin complex seems to associate with a complex containing the phosphorylated receptor (EGFR or MET) and phosphorylated CBL. Probably associates with ASAP1 and phosphorylated EGFR. Probably part of a complex consisting of at least SH3KBP1, ASAP1 and ARAP3. Interacts with focal adhesion kinases PTK2 AND PTK2B, probably as a dimer. Interacts with DAB2 and probably associates with chathrin through its interaction with DAB2. Part of a complex consisting of SH3KBP1, DAB2, and clathrin heavy chain. DAB2 and clathrin dissociate from SH3KBP1 following growth factor treatment, enabling interaction with CBL. Interacts with DDN and probably associates with MAGI2 through its interaction with DDN. Interacts with the SH3 domains of SRC tyrosine-protein kinases SRC, LCK, LYN, FGR, FYN and HCK. Interacts with TRADD, BIRC2, TRAF1, TRAF2 and TNFR1, and the association with a TNFR1-associated complex upon stimulation with TNF-alpha seems to be mediated by SRC. Probably interacts with SH3KBP1. 2 isoforms of the human protein are produced by alternative splicing. |
| UniProt Protein Details: | Protein type:Apoptosis; Adaptor/scaffold; Motility/polarity/chemotaxis Chromosomal Location of Human Ortholog: Xp22.1-p21.3 Cellular Component: neuron projection; cytoskeleton; cytoplasmic vesicle membrane; focal adhesion; plasma membrane; synapse; intercellular junction; cytosol Molecular Function:protein binding; SH3 domain binding Biological Process: epidermal growth factor receptor signaling pathway; negative regulation of epidermal growth factor receptor signaling pathway; regulation of cell shape; cell migration; cell-cell signaling; apoptosis; cytoskeleton organization and biogenesis; endocytosis |
| NCBI Summary: | This gene encodes an adapter protein that contains three N-terminal Src homology domains, a proline rich region and a C-terminal coiled-coil domain. The encoded protein facilitates protein-protein interactions and has been implicated in numerous cellular processes including apoptosis, cytoskeletal rearrangement, cell adhesion and in the regulation of clathrin-dependent endocytosis. Alternate splicing results in multiple transcript variants.[provided by RefSeq, May 2010] |
| UniProt Code: | Q96B97 |
| NCBI GenInfo Identifier: | 31077034 |
| NCBI Gene ID: | 30011 |
| NCBI Accession: | Q96B97.2 |
| UniProt Secondary Accession: | Q96B97,Q5JPT4, Q5JPT5, Q8IWX6, Q8IX98, Q96RN4, Q9NYR0 B7Z1D5, |
| UniProt Related Accession: | Q96B97 |
| Molecular Weight: | 665 |
| NCBI Full Name: | SH3 domain-containing kinase-binding protein 1 |
| NCBI Synonym Full Names: | SH3-domain kinase binding protein 1 |
| NCBI Official Symbol: | SH3KBP1 |
| NCBI Official Synonym Symbols: | HSB1; CIN85; GIG10; HSB-1; MIG18; CD2BP3 |
| NCBI Protein Information: | SH3 domain-containing kinase-binding protein 1; CD2-binding protein 3; c-Cbl-interacting protein; migration-inducing gene 18; cbl-interacting protein of 85 kDa; Src family kinase-binding protein 1; src-related kinase binding protein-1; human Src family ki |
| UniProt Protein Name: | SH3 domain-containing kinase-binding protein 1 |
| UniProt Synonym Protein Names: | CD2-binding protein 3; CD2BP3; Cbl-interacting protein of 85 kDa; Human Src family kinase-binding protein 1; HSB-1 |
| Protein Family: | Sugar efflux transporter |
| UniProt Gene Name: | SH3KBP1 |
| UniProt Entry Name: | SH3K1_HUMAN |
 | Western blot analysis of extracts of THP-1 cells, using SH3KBP1 antibody (CAB1952) at 1:1000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (CABS014) at 1:10000 dilution. Lysates/proteins: 25ug per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit (RM00020). Exposure time: 90s. |
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