Cell Biology Antibodies 9
Anti-SH3GL2 Antibody (CAB5701)
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- SKU:
- CAB5701
- Product Type:
- Antibody
- Reactivity:
- Human
- Reactivity:
- Mouse
- Host Species:
- Rabbit
- Isotype:
- IgG
- Antibody Type:
- Polyclonal Antibody
- Research Area:
- Cell Biology
Description
| Antibody Name: | Anti-SH3GL2 Antibody |
| Antibody SKU: | CAB5701 |
| Antibody Size: | 20uL, 50uL, 100uL |
| Application: | WB IF |
| Reactivity: | Human, Mouse |
| Host Species: | Rabbit |
| Immunogen: | Recombinant fusion protein containing a sequence corresponding to amino acids 1-270 of human SH3GL2 (NP_003017.1). |
| Application: | WB IF |
| Recommended Dilution: | WB 1:500 - 1:2000 IF 1:50 - 1:200 |
| Reactivity: | Human, Mouse |
| Positive Samples: | HeLa, BT-474, 293T, HepG2, Mouse brain, Mouse testis |
| Immunogen: | Recombinant fusion protein containing a sequence corresponding to amino acids 1-270 of human SH3GL2 (NP_003017.1). |
| Purification Method: | Affinity purification |
| Storage Buffer: | Store at -20'C. Avoid freeze / thaw cycles. Buffer: PBS with 0.02% sodium azide, 50% glycerol, pH7.3. |
| Isotype: | IgG |
| Sequence: | MSVA GLKK QFHK ATQK VSEK VGGA EGTK LDDD FKEM ERKV DVTS RAVM EIMT KTIE YLQP NPAS RAKL SMIN TMSK IRGQ EKGP GYPQ AEAL LAEA MLKF GREL GDDC NFGP ALGE VGEA MREL SEVK DSLD IEVK QNFI DPLQ NLHD KDLR EIQH HLKK LEGR RLDF DYKK KRQG KIPD EELR QALE KFDE SKEI AESS MFNL LEMD IEQV SQLS ALVQ AQLE YHKQ AVQI LQQV TVRL EERI RQAS SQPR REYQ PKPR MSLE FPTG DS |
| Gene ID: | 6456 |
| Uniprot: | Q99962 |
| Cellular Location: | Cytoplasm, Membrane, Peripheral membrane protein |
| Calculated MW: | 39kDa |
| Observed MW: | 38kDa/40kDa |
| Synonyms: | SH3GL2, CNSA2, EEN-B1, SH3D2A, SH3P4 |
| Background: |
| UniProt Protein Function: | endophilin 1: Implicated in synaptic vesicle endocytosis. May recruit other proteins to membranes with high curvature. Belongs to the endophilin family. |
| UniProt Protein Details: | Protein type:Adaptor/scaffold; Membrane protein, peripheral; Vesicle Chromosomal Location of Human Ortholog: 9p22 Cellular Component: cytosol; Golgi membrane; plasma membrane Molecular Function:identical protein binding; protein binding Biological Process: antigen processing and presentation of exogenous peptide antigen via MHC class II; central nervous system development; microtubule-based movement; negative regulation of epidermal growth factor receptor signaling pathway; signal transduction |
| UniProt Code: | Q99962 |
| NCBI GenInfo Identifier: | 10720276 |
| NCBI Gene ID: | 6456 |
| NCBI Accession: | Q99962.1 |
| UniProt Secondary Accession: | Q99962,Q9NQK5, B2R618, |
| UniProt Related Accession: | Q99962 |
| Molecular Weight: | 39,962 Da |
| NCBI Full Name: | Endophilin-A1 |
| NCBI Synonym Full Names: | SH3 domain containing GRB2 like 2, endophilin A1 |
| NCBI Official Symbol: | SH3GL2 |
| NCBI Official Synonym Symbols: | CNSA2; SH3P4; EEN-B1; SH3D2A |
| NCBI Protein Information: | endophilin-A1 |
| UniProt Protein Name: | Endophilin-A1 |
| UniProt Synonym Protein Names: | EEN-B1; Endophilin-1; SH3 domain protein 2A; SH3 domain-containing GRB2-like protein 2 |
| Protein Family: | Endophilin |
| UniProt Gene Name: | SH3GL2 |
| UniProt Entry Name: | SH3G2_HUMAN |
 | Western blot analysis of extracts of various cell lines, using SH3GL2 antibody (CAB5701) at 1:1000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (CABS014) at 1:10000 dilution. Lysates/proteins: 25ug per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit (RM00020). Exposure time: 90s. |
 | Immunofluorescence analysis of HeLa cells using SH3GL2 antibody (CAB5701). Blue: DAPI for nuclear staining. |
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