KO Validated Antibodies 2

Anti-SH3GL1 Antibody (CAB19932)[KO Validated]

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SKU:
CAB19932
Product Type:
Antibody
Reactivity:
Human
Reactivity:
Mouse
Reactivity:
Rat
Reactivity:
Monkey
Host Species:
Rabbit
Isotype:
IgG
Research Area:
Cell Biology

Description

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Antibody Name:Anti-SH3GL1 Antibody [KO Validated]
Antibody SKU:CAB19932
Antibody Size:20uL, 50uL, 100uL
Application:WB IHC
Reactivity:Human, Mouse, Rat, Monkey
Host Species:Rabbit
Immunogen:Recombinant protein of human SH3GL1.
Application:WB IHC
Recommended Dilution:WB 1:500 - 1:2000 IHC 1:100 - 1:200
Reactivity:Human, Mouse, Rat, Monkey
Positive Samples:293T
Immunogen:Recombinant protein of human SH3GL1.
Purification Method:Affinity purification
Storage Buffer:Store at -20°C. Avoid freeze / thaw cycles. Buffer: PBS with 0.02% sodium azide, 50% glycerol, pH7.3.
Isotype:IgG
Sequence:Email for sequence
Gene ID:6455
Uniprot:Q99961
Cellular Location:Cell projection, Cytoplasm, Early endosome membrane, Peripheral membrane protein, podosome
Calculated MW:34kDa/36kDa/41kDa
Observed MW:45KDa
Anti-SH3GL1 Antibody [KO Validated] (CAB19932)Western blot analysis of extracts from normal (control) and SH3GL1 knockout (KO) 293T cells, using SH3GL1 antibody (CAB19932) at 1:1000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (CABS014) at 1:10000 dilution. Lysates/proteins: 25ug per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit (RM00020). Exposure time: 1s.
Synonyms:SH3GL1, CNSA1, EEN, SH3D2B, SH3P8
Background:This gene encodes a member of the endophilin family of Src homology 3 domain-containing proteins. The encoded protein is involved in endocytosis and may also play a role in the cell cycle. Overexpression of this gene may play a role in leukemogenesis, and the encoded protein has been implicated in acute myeloid leukemia as a fusion partner of the myeloid-lymphoid leukemia protein. Pseudogenes of this gene are located on the long arm of chromosomes 11 and 17. Alternatively spliced transcript variants encoding multiple isoforms have been observed for this gene.
UniProt Protein Function:SH3GL1: Implicated in endocytosis. May recruit other proteins to membranes with high curvature. In some cases of acute leukemia, a translocation results in the formation of a MLL-EEN fusion gene. Belongs to the endophilin family.
UniProt Protein Details:

Protein type:Oncoprotein; Vesicle

Chromosomal Location of Human Ortholog: 19p13.3

Cellular Component: cell projection; early endosome membrane; cytoplasm; podosome; cell junction

Molecular Function:identical protein binding; protein binding; GTPase binding; lipid binding; phosphatase binding

Biological Process: central nervous system development; endocytosis; signal transduction

Disease: Leukemia, Acute Myeloid

NCBI Summary:This gene encodes a member of the endophilin family of Src homology 3 domain-containing proteins. The encoded protein is involved in endocytosis and may also play a role in the cell cycle. Overexpression of this gene may play a role in leukemogenesis, and the encoded protein has been implicated in acute myeloid leukemia as a fusion partner of the myeloid-lymphoid leukemia protein. Pseudogenes of this gene are located on the long arm of chromosomes 11 and 17. Alternatively spliced transcript variants encoding multiple isoforms have been observed for this gene. [provided by RefSeq, Jan 2011]
UniProt Code:Q99961
NCBI GenInfo Identifier:12643797
NCBI Gene ID:6455
NCBI Accession:Q99961.1
UniProt Secondary Accession:Q99961,Q99668, B4DRA1, E7EVZ4, M0QZV5,
UniProt Related Accession:Q99961
Molecular Weight:368
NCBI Full Name:Endophilin-A2
NCBI Synonym Full Names:SH3-domain GRB2-like 1
NCBI Official Symbol:SH3GL1  
NCBI Official Synonym Symbols:EEN; CNSA1; SH3P8; SH3D2B  
NCBI Protein Information:endophilin-A2; endophilin-2; SH3 domain protein 2B; EEN fusion partner of MLL; extra 11-19 leukemia fusion; SH3-containing Grb-2-like 1 protein; SH3 domain-containing GRB2-like protein 1; extra eleven-nineteen leukemia fusion gene protein
UniProt Protein Name:Endophilin-A2
UniProt Synonym Protein Names:EEN fusion partner of MLL; Endophilin-2; Extra eleven-nineteen leukemia fusion gene protein; EEN; SH3 domain protein 2B; SH3 domain-containing GRB2-like protein 1
Protein Family:Endophilin
UniProt Gene Name:SH3GL1  
UniProt Entry Name:SH3G1_HUMAN
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