KO Validated Antibodies 1

Anti-SGTA Antibody (CAB7306)[KO Validated]

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SKU:
CAB7306
Product Type:
Antibody
Reactivity:
Human
Host Species:
Rabbit
Isotype:
IgG
Antibody Type:
Polyclonal Antibody
Research Area:
Immunology

Description

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Antibody Name:Anti-SGTA Antibody [KO Validated]
Antibody SKU:CAB7306
Antibody Size:20uL, 50uL, 100uL
Application:WB IHC IF
Reactivity:Human
Host Species:Rabbit
Immunogen:Recombinant fusion protein containing a sequence corresponding to amino acids 1-120 of human SGTA (NP_003012.1).
Application:WB IHC IF
Recommended Dilution:WB 1:500 - 1:2000 IHC 1:50 - 1:200 IF 1:50 - 1:100
Reactivity:Human
Positive Samples:HeLa, A-549, U-251MG
Immunogen:Recombinant fusion protein containing a sequence corresponding to amino acids 1-120 of human SGTA (NP_003012.1).
Purification Method:Affinity purification
Storage Buffer:Store at -20'C. Avoid freeze / thaw cycles. Buffer: PBS with 0.02% sodium azide, 50% glycerol, pH7.3.
Isotype:IgG
Sequence:MDNK KRLA YAII QFLH DQLR HGGL SSDA QESL EVAI QCLE TAFG VTVE DSDL ALPQ TLPE IFEA AATG KEMP QDLR SPAR TPPS EEDS AEAE RLKT EGNE QMKV ENFE AAVH FYGK AIEL
Gene ID:6449
Uniprot:O43765
Cellular Location:
Calculated MW:34kDa
Observed MW:38kDa
Synonyms:SGTA, SGT, alphaSGT, hSGT
Background:This gene encodes a protein which is capable of interacting with the major nonstructural protein of parvovirus H-1 and 70-kDa heat shock cognate protein; however, its function is not known. Since this transcript is expressed ubiquitously in various tissues, this protein may serve a housekeeping function.
UniProt Protein Function:SGTA: Co-chaperone that binds directly to HSC70 and HSP70 and regulates their ATPase activity. Homooligomerize. Interacts with NS1 from parvovirus H-1, with Vpu and Gag from HIV-1. Interacts with SARS- CoV accessory protein 7a. Interacts with DNAJC5 and DNAJC5B. Ubiquitous. Belongs to the SGT family.
UniProt Protein Details:

Protein type:Chaperone

Chromosomal Location of Human Ortholog: 19p13

Cellular Component: cytoplasm

Molecular Function:protein binding

Biological Process: viral reproduction

NCBI Summary:This gene encodes a protein which is capable of interacting with the major nonstructural protein of parvovirus H-1 and 70-kDa heat shock cognate protein; however, its function is not known. Since this transcript is expressed ubiquitously in various tissues, this protein may serve a housekeeping function. [provided by RefSeq, Jul 2008]
UniProt Code:O43765
NCBI GenInfo Identifier:8134666
NCBI Gene ID:6449
NCBI Accession:O43765.1
UniProt Related Accession:O43765
Molecular Weight:
NCBI Full Name:Small glutamine-rich tetratricopeptide repeat-containing protein alpha
NCBI Synonym Full Names:small glutamine rich tetratricopeptide repeat containing alpha
NCBI Official Symbol:SGTA  
NCBI Official Synonym Symbols:SGT; hSGT; alphaSGT  
NCBI Protein Information:small glutamine-rich tetratricopeptide repeat-containing protein alpha
UniProt Protein Name:Small glutamine-rich tetratricopeptide repeat-containing protein alpha
UniProt Synonym Protein Names:Alpha-SGT; Vpu-binding protein; UBP
Protein Family:Small glutamine-rich tetratricopeptide repeat-containing protein
UniProt Gene Name:SGTA  
UniProt Entry Name:SGTA_HUMAN
Anti-SGTA Antibody [KO Validated] (CAB7306)Western blot analysis of extracts of various cell lines, using SGTA antibody (CAB7306) at 1:1000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (CABS014) at 1:10000 dilution. Lysates/proteins: 25ug per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit (RM00020). Exposure time: 90s.
Anti-SGTA Antibody [KO Validated] (CAB7306)Western blot analysis of extracts from normal (control) and SGTA knockout (KO) 293T cells, using SGTA antibody (CAB7306) at 1:1000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (CABS014) at 1:10000 dilution. Lysates/proteins: 25ug per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit (RM00020). Exposure time: 30s.
Anti-SGTA Antibody [KO Validated] (CAB7306)Immunohistochemistry of paraffin-embedded human liver cancer using SGTA antibody (CAB7306) at dilution of 1:100 (40x lens).
Anti-SGTA Antibody [KO Validated] (CAB7306)Immunofluorescence analysis of A-549 cells using SGTA antibody (CAB7306). Blue: DAPI for nuclear staining.
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