Antibody Name:	Anti-SGOL1 Antibody
Antibody SKU:	CAB16174
Antibody Size:	20uL, 50uL, 100uL
Application:	WB IF
Reactivity:	Human, Mouse, Rat
Host Species:	Rabbit
Immunogen:	Recombinant fusion protein containing a sequence corresponding to amino acids 10-100 of human SGOL1 (NP_612493.1).

Application:	WB IF
Recommended Dilution:	WB 1:500 - 1:2000 IF 1:50 - 1:200
Reactivity:	Human, Mouse, Rat
Positive Samples:	A-549, HT-29, Mouse testis, Rat testis

Immunogen:	Recombinant fusion protein containing a sequence corresponding to amino acids 10-100 of human SGOL1 (NP_612493.1).
Purification Method:	Affinity purification
Storage Buffer:	Store at -20'C. Avoid freeze / thaw cycles. Buffer: PBS with 0.02% sodium azide, 50% glycerol, pH7.3.
Isotype:	IgG
Sequence:	SFQD SLED IKKR MKEK RNKN LAEI GKRR SFIA APCQ IITN TSTL LKNY QDNN KMLV LALE NEKS KVKE AQDI ILQL RKEC YYLT CQLY ALK
Gene ID:	151648
Uniprot:	Q5FBB7
Cellular Location:	Chromosome, Cytoplasm, Nucleus, centromere, centrosome, cytoskeleton, kinetochore, microtubule organizing center, spindle pole
Calculated MW:	24kDa/29kDa/31kDa/33kDa/35kDa/60kDa/64kDa
Observed MW:	75kDa

Synonyms:

SGO1, CAID, NY-BR-85, SGO, SGOL1

Background:

The protein encoded by this gene is a member of the shugoshin family of proteins. This protein is thought to protect centromeric cohesin from cleavage during mitotic prophase by preventing phosphorylation of a cohesin subunit. Reduced expression of this gene leads to the premature loss of centromeric cohesion, mis-segregation of sister chromatids, and mitotic arrest. Evidence suggests that this protein also protects a small subset of cohesin found along the length of the chromosome arms during mitotic prophase. An isoform lacking exon 6 has been shown to play a role in the cohesion of centrioles (PMID: 16582621 and PMID:18331714). Mutations in this gene have been associated with Chronic Atrial and Intestinal Dysrhythmia (CAID) syndrome, characterized by the co-occurrence of Sick Sinus Syndrome (SSS) and Chronic Intestinal Pseudo-obstruction (CIPO) within the first four decades of life (PMID:25282101). Fibroblast cells from CAID patients exhibited both increased cell proliferation and higher rates of senescence. Pseudogenes of this gene have been found on chromosomes 1 and 7. Alternative splicing results in multiple transcript variants.

UniProt Protein Function:	SGO1: Plays a central role in chromosome cohesion during mitosis by preventing premature dissociation of cohesin complex from centromeres after prophase, when most of cohesin complex dissociates from chromosomes arms. May act by preventing phosphorylation of the STAG2 subunit of cohesin complex at the centromere, ensuring cohesin persistence at centromere until cohesin cleavage by ESPL1/separase at anaphase. Essential for proper chromosome segregation during mitosis and this function requires interaction with PPP2R1A. Its phosphorylated form is necessary for chromosome congression and for the proper attachment of spindle microtubule to the kinetochore. Necessary for kinetochore localization of PLK1 and CENPF. May play a role in the tension sensing mechanism of the spindle-assembly checkpoint by regulating PLK1 kinetochore affinity. Isoform 3 plays a role in maintaining centriole cohesion involved in controlling spindle pole integrity. Belongs to the shugoshin family. 7 isoforms of the human protein are produced by alternative splicing.
UniProt Protein Details:	Protein type:Cell cycle regulation Chromosomal Location of Human Ortholog: 3p24.3 Cellular Component: centrosome; chromosome, pericentric region; cytoplasm; cytosol; kinetochore; nucleoplasm; spindle pole Molecular Function:kinase binding; protein binding Biological Process: attachment of spindle microtubules to kinetochore; centriole-centriole cohesion; chromosome segregation; meiotic chromosome segregation; sister chromatid cohesion Disease: Chronic Atrial And Intestinal Dysrhythmia
NCBI Summary:	The protein encoded by this gene is a member of the shugoshin family of proteins. This protein is thought to protect centromeric cohesin from cleavage during mitotic prophase by preventing phosphorylation of a cohesin subunit. Reduced expression of this gene leads to the premature loss of centromeric cohesion, mis-segregation of sister chromatids, and mitotic arrest. Evidence suggests that this protein also protects a small subset of cohesin found along the length of the chromosome arms during mitotic prophase. An isoform lacking exon 6 has been shown to play a role in the cohesion of centrioles (PMID: 16582621 and PMID:18331714). Mutations in this gene have been associated with Chronic Atrial and Intestinal Dysrhythmia (CAID) syndrome, characterized by the co-occurrence of Sick Sinus Syndrome (SSS) and Chronic Intestinal Pseudo-obstruction (CIPO) within the first four decades of life (PMID:25282101). Fibroblast cells from CAID patients exhibited both increased cell proliferation and higher rates of senescence. Pseudogenes of this gene have been found on chromosomes 1 and 7. Alternative splicing results in multiple transcript variants. [provided by RefSeq, Mar 2015]
UniProt Code:	Q5FBB7
NCBI GenInfo Identifier:	74741474
NCBI Gene ID:	151648
NCBI Accession:	Q5FBB7.1
UniProt Secondary Accession:	Q5FBB7,Q588H5, Q5FBB4, Q5FBB5, Q5FBB6, Q5FBB8, Q8N579 Q8WVL0, Q9BVA8, Q9H275,
UniProt Related Accession:	Q5FBB7
Molecular Weight:	24,646 Da
NCBI Full Name:	Shugoshin 1
NCBI Synonym Full Names:	shugoshin 1
NCBI Official Symbol:	SGO1
NCBI Official Synonym Symbols:	SGO; CAID; SGOL1; NY-BR-85
NCBI Protein Information:	shugoshin 1
UniProt Protein Name:	Shugoshin 1
UniProt Synonym Protein Names:	Serologically defined breast cancer antigen NY-BR-85; Shugoshin-like 1
UniProt Gene Name:	SGO1
UniProt Entry Name:	SGO1_HUMAN

	Western blot analysis of extracts of various cell lines, using SGOL1 antibody (CAB16174) at 1:1000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (CABS014) at 1:10000 dilution. Lysates/proteins: 25ug per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit (RM00020). Exposure time: 3s.
	Immunofluorescence analysis of U20S cells using SGOL1 antibody (CAB16174) at dilution of 1:100. Blue: DAPI for nuclear staining.