Name: Anti-SGK3 Antibody (CAB7586)
Brand: Cell Biology Antibodies 11
SKU: CAB7586
Availability: OutOfStock

Antibody Name:	Anti-SGK3 Antibody
Antibody SKU:	CAB7586
Antibody Size:	20uL, 50uL, 100uL
Application:	WB
Reactivity:	Human, Mouse
Host Species:	Rabbit
Immunogen:	Recombinant fusion protein containing a sequence corresponding to amino acids 1-150 of human SGK3 (NP_037389.4).

Application:	WB
Recommended Dilution:	WB 1:500 - 1:2000
Reactivity:	Human, Mouse
Positive Samples:	HL-60, THP-1, Mouse kidney

Immunogen:	Recombinant fusion protein containing a sequence corresponding to amino acids 1-150 of human SGK3 (NP_037389.4).
Purification Method:	Affinity purification
Storage Buffer:	Store at -20'C. Avoid freeze / thaw cycles. Buffer: PBS with 0.02% sodium azide, 50% glycerol, pH7.3.
Isotype:	IgG
Sequence:	MQRD HTMD YKES CPSV SIPS SDEH REKK KRFT VYKV LVSV GRSE WFVF RRYA EFDK LYNT LKKQ FPAM ALKI PAKR IFGD NFDP DFIK QRRA GLNE FIQN LVRY PELY NHPD VRAF LQMD SPKH QSDP SEDE DERS SQKL HSTS QNIN LG
Gene ID:	23678
Uniprot:	Q96BR1
Cellular Location:	Cytoplasmic vesicle, Early endosome, Recycling endosome
Calculated MW:	53kDa/57kDa
Observed MW:	57kDa

Synonyms:	SGK3, CISK, SGK2, SGKL
Background:	This gene is a member of the Ser/Thr protein kinase family and encodes a phosphoprotein with a PX (phox homology) domain. The protein phosphorylates several target proteins and has a role in neutral amino acid transport and activation of potassium and chloride channels. Alternate transcriptional splice variants, encoding different isoforms, have been characterized.

UniProt Protein Function:	SGK3: Serine/threonine-protein kinase which is involved in the regulation of a wide variety of ion channels, membrane transporters, cell growth, proliferation, survival and migration. Up-regulates Na(+) channels: SCNN1A/ENAC and SCN5A, K(+) channels: KCNA3/KV1.3, KCNE1, KCNQ1 and KCNH2/HERG, epithelial Ca(2+) channels: TRPV5 and TRPV6, chloride channel: BSND, creatine transporter: SLC6A8, Na(+)/dicarboxylate cotransporter: SLC13A2/NADC1, Na(+)-dependent phosphate cotransporter: SLC34A2/NAPI-2B, amino acid transporters: SLC1A5/ASCT2 and SLC6A19, glutamate transporters: SLC1A3/EAAT1, SLC1A6/EAAT4 and SLC1A7/EAAT5, glutamate receptors: GRIA1/GLUR1 and GRIK2/GLUR6, Na(+)/H(+) exchanger: SLC9A3/NHE3, and the Na(+)/K(+) ATPase. Plays a role in the regulation of renal tubular phosphate transport and bone density. Phosphorylates NEDD4L and GSK3B. Positively regulates ER transcription activity through phosphorylation of FLII. Negatively regulates the function of ITCH/AIP4 via its phosphorylation and thereby prevents CXCR4 from being efficiently sorted to lysosomes. Interacts with GSK3B and FLII. Interacts with PDPK1 in a phosphorylation-dependent manner. Induced by estrogen/ER in breast cancer cells. Expressed in most tissues with highest levels in pancreas, kidney liver, heart and brain and lower levels in lung, placenta and skeletal muscle. Expression is higher in ER- positive breast tumors than ER-negative breast tumors. Two specific sites, one in the kinase domain (Thr-320) and the other in the C-terminal regulatory region (Ser- 486), need to be phosphorylated for its full activation. Belongs to the protein kinase superfamily. AGC Ser/Thr protein kinase family. 2 isoforms of the human protein are produced by alternative splicing.
UniProt Protein Details:	Protein type:Kinase, protein; EC 2.7.11.1; Protein kinase, Ser/Thr (non-receptor); Protein kinase, AGC; AGC group; SGK family Chromosomal Location of Human Ortholog: 8q12 Cellular Component: cytoplasmic membrane-bound vesicle; cytosol; early endosome; recycling endosome Molecular Function:ATP binding; calcium channel regulator activity; chloride channel regulator activity; phosphoinositide binding; potassium channel regulator activity; protein binding; protein kinase activity; protein serine/threonine kinase activity; sodium channel regulator activity Biological Process: cellular sodium ion homeostasis; peptidyl-serine phosphorylation; positive regulation of transporter activity; protein amino acid phosphorylation; regulation of apoptosis; regulation of cell growth; regulation of cell migration; regulation of cell proliferation; regulation of transcription factor activity; response to stress
NCBI Summary:	This gene is a member of the Ser/Thr protein kinase family and encodes a phosphoprotein with a PX (phox homology) domain. The protein phosphorylates several target proteins and has a role in neutral amino acid transport and activation of potassium and chloride channels. Alternate transcriptional splice variants, encoding different isoforms, have been characterized. [provided by RefSeq, Jul 2008]
UniProt Code:	Q96BR1
NCBI GenInfo Identifier:	28558163
NCBI Gene ID:	23678
NCBI Accession:	Q96BR1.1
UniProt Secondary Accession:	Q96BR1,Q9P1Q7, Q9UKG5, A8K5W3, B3KQC2,
UniProt Related Accession:	Q96BR1
Molecular Weight:	53,306 Da
NCBI Full Name:	Serine/threonine-protein kinase Sgk3
NCBI Synonym Full Names:	serum/glucocorticoid regulated kinase family member 3
NCBI Official Symbol:	SGK3
NCBI Official Synonym Symbols:	CISK; SGK2; SGKL
NCBI Protein Information:	serine/threonine-protein kinase Sgk3
UniProt Protein Name:	Serine/threonine-protein kinase Sgk3
UniProt Synonym Protein Names:	Cytokine-independent survival kinase; Serum/glucocorticoid-regulated kinase 3; Serum/glucocorticoid-regulated kinase-like
Protein Family:	Serine/threonine-protein kinase
UniProt Gene Name:	SGK3
UniProt Entry Name:	SGK3_HUMAN

Western blot analysis of extracts of various cell lines, using SGK3 antibody (CAB7586) at 1:1000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (CABS014) at 1:10000 dilution. Lysates/proteins: 25ug per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Enhanced Kit (RM00021). Exposure time: 10s.

Antibodies

SGK3 Antibody (PACO12166)

Secondary Antibody

Anti-HRP Goat Anti-Rabbit IgG (H+L) Antibody (CABS014)