Name: Anti-SGCG Antibody (CAB10540)
Brand: Cell Biology Antibodies 2
SKU: CAB10540
Availability: OutOfStock

Antibody Name:	Anti-SGCG Antibody
Antibody SKU:	CAB10540
Antibody Size:	20uL, 50uL, 100uL
Application:	WB
Reactivity:	Human
Host Species:	Rabbit
Immunogen:	Recombinant fusion protein containing a sequence corresponding to amino acids 72-291 of human SGCG (NP_000222.1).

Application:	WB
Recommended Dilution:	WB 1:1000 - 1:2000
Reactivity:	Human
Positive Samples:	HeLa, MCF7

Immunogen:	Recombinant fusion protein containing a sequence corresponding to amino acids 72-291 of human SGCG (NP_000222.1).
Purification Method:	Affinity purification
Storage Buffer:	Store at -20'C. Avoid freeze / thaw cycles. Buffer: PBS with 0.02% sodium azide, 50% glycerol, pH7.3.
Isotype:	IgG
Sequence:	CVTK DGLR LEGE SEFL FPLY AKEI HSRV DSSL LLQS TQNV TVNA RNSE GEVT GRLK VGPK MVEV QNQQ FQIN SNDG KPLF TVDE KEVV VGTD KLRV TGPE GALF EHSV ETPL VRAD PFQD LRLE SPTR SLSM DAPR GVHI QAHA GKIE ALSQ MDIL FHSS DGML VLDA ETVC LPKL VQGT WGPS GSSQ SLYE ICVC PDGK LYLS VAGV STTC QEHS HICL
Gene ID:	6445
Uniprot:	Q13326
Cellular Location:	Cell membrane, Cytoplasm, Single-pass type II membrane protein, cytoskeleton, sarcolemma
Calculated MW:	32kDa
Observed MW:	37kDa

Synonyms:

SGCG, 35DAG, A4, DAGA4, DMDA, DMDA1, LGMD2C, MAM, SCARMD2, SCG3, gamma-SG

Background:

This gene encodes gamma-sarcoglycan, one of several sarcolemmal transmembrane glycoproteins that interact with dystrophin. The dystrophin-glycoprotein complex (DGC) spans the sarcolemma and is comprised of dystrophin, syntrophin, alpha- and beta-dystroglycans and sarcoglycans. The DGC provides a structural link between the subsarcolemmal cytoskeleton and the extracellular matrix of muscle cells. Defects in the encoded protein can lead to early onset autosomal recessive muscular dystrophy, in particular limb-girdle muscular dystrophy, type 2C (LGMD2C).

UniProt Protein Function:	SGCG: Component of the sarcoglycan complex, a subcomplex of the dystrophin-glycoprotein complex which forms a link between the F-actin cytoskeleton and the extracellular matrix. Defects in SGCG are the cause of limb-girdle muscular dystrophy type 2C (LGMD2C). LGMD2C is characterized by progressive muscle wasting from early childhood. Belongs to the sarcoglycan beta/delta/gamma/zeta family.
UniProt Protein Details:	Protein type:Membrane protein, integral; Dystrophin complex Chromosomal Location of Human Ortholog: 13q12 Cellular Component: cytoskeleton; cytoplasm; plasma membrane; integral to membrane; sarcoglycan complex; sarcolemma Molecular Function:protein binding Biological Process: cardiac muscle development; muscle development; heart contraction; muscle cell development Disease: Muscular Dystrophy, Limb-girdle, Type 2c
NCBI Summary:	This gene encodes gamma-sarcoglycan, one of several sarcolemmal transmembrane glycoproteins that interact with dystrophin. The dystrophin-glycoprotein complex (DGC) spans the sarcolemma and is comprised of dystrophin, syntrophin, alpha- and beta-dystroglycans and sarcoglycans. The DGC provides a structural link between the subsarcolemmal cytoskeleton and the extracellular matrix of muscle cells. Defects in the encoded protein can lead to early onset autosomal recessive muscular dystrophy, in particular limb-girdle muscular dystrophy, type 2C (LGMD2C). [provided by RefSeq, Oct 2008]
UniProt Code:	Q13326
NCBI GenInfo Identifier:	313104319
NCBI Gene ID:	6445
NCBI Accession:	Q13326.4
UniProt Secondary Accession:	Q13326,Q32M32, Q5T9J6,
UniProt Related Accession:	Q13326
Molecular Weight:	291
NCBI Full Name:	Gamma-sarcoglycan
NCBI Synonym Full Names:	sarcoglycan, gamma (35kDa dystrophin-associated glycoprotein)
NCBI Official Symbol:	SGCG
NCBI Official Synonym Symbols:	A4; MAM; DMDA; SCG3; TYPE; DAGA4; DMDA1; LGMD2C; SCARMD2
NCBI Protein Information:	gamma-sarcoglycan; 35DAG; gamma-SG; gamma sarcoglycan; 35kD dystrophin-associated glycoprotein; 35 kDa dystrophin-associated glycoprotein
UniProt Protein Name:	Gamma-sarcoglycan
UniProt Synonym Protein Names:	35 kDa dystrophin-associated glycoprotein; 35DAG
Protein Family:	Gamma-sarcoglycan
UniProt Gene Name:	SGCG
UniProt Entry Name:	SGCG_HUMAN

Western blot analysis of extracts of various cell lines, using SGCG antibody (CAB10540) at 1:1000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (CABS014) at 1:10000 dilution. Lysates/proteins: 25ug per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit (RM00020). Exposure time: 10s.

Antibodies

SGCG Antibody (PACO12164)

Secondary Antibody

Anti-HRP Goat Anti-Rabbit IgG (H+L) Antibody (CABS014)