Antibody Name:	Anti-SFRS9 Antibody
Antibody SKU:	CAB6070
Antibody Size:	20uL, 50uL, 100uL
Application:	WB IHC IF
Reactivity:	Human, Mouse
Host Species:	Rabbit
Immunogen:	Recombinant fusion protein containing a sequence corresponding to amino acids 1-221 of human SFRS9 (NP_003760.1).

Application:	WB IHC IF
Recommended Dilution:	WB 1:500 - 1:1000 IHC 1:50 - 1:200 IF 1:50 - 1:200
Reactivity:	Human, Mouse
Positive Samples:	BT-474, HepG2, Jurkat, Mouse testis

Immunogen:	Recombinant fusion protein containing a sequence corresponding to amino acids 1-221 of human SFRS9 (NP_003760.1).
Purification Method:	Affinity purification
Storage Buffer:	Store at -20'C. Avoid freeze / thaw cycles. Buffer: PBS with 0.02% sodium azide, 50% glycerol, pH7.3.
Isotype:	IgG
Sequence:	MSGW ADER GGEG DGRI YVGN LPTD VREK DLED LFYK YGRI REIE LKNR HGLV PFAF VRFE DPRD AEDA IYGR NGYD YGQC RLRV EFPR TYGG RGGW PRGG RNGP PTRR SDFR VLVS GLPP SGSW QDLK DHMR EAGD VCYA DVQK DGVG MVEY LRKE DMEY ALRK LDDT KFRS HEGE TSYI RVYP ERST SYGY SRSR SGSR GRDS PYQS RGSP HYFS PFRP Y
Gene ID:	8683
Uniprot:	Q13242
Cellular Location:	Nucleus
Calculated MW:	25kDa
Observed MW:	26kDa

Synonyms:

SRSF9, SFRS9, SRp30c

Background:

The protein encoded by this gene is a member of the serine/arginine (SR)-rich family of pre-mRNA splicing factors, which constitute part of the spliceosome. Each of these factors contains an RNA recognition motif (RRM) for binding RNA and an RS domain for binding other proteins. The RS domain is rich in serine and arginine residues and facilitates interaction between different SR splicing factors. In addition to being critical for mRNA splicing, the SR proteins have also been shown to be involved in mRNA export from the nucleus and in translation. Two pseudogenes, one on chromosome 15 and the other on chromosome 21, have been found for this gene.

UniProt Protein Function:	SFRS9: Plays a role in constitutive splicing and can modulate the selection of alternative splice sites. Represses the splicing of MAPT/Tau exon 10. Belongs to the splicing factor SR family.
UniProt Protein Details:	Protein type:RNA-binding; Spliceosome; RNA splicing Chromosomal Location of Human Ortholog: 12q24.31 Cellular Component: nucleoplasm; nucleolus Molecular Function:protein binding; nucleotide binding Biological Process: transcription from RNA polymerase II promoter; nuclear mRNA splicing, via spliceosome; mRNA splice site selection; mRNA export from nucleus; negative regulation of nuclear mRNA splicing, via spliceosome; RNA splicing; gene expression; mRNA 3'-end processing; mRNA processing; termination of RNA polymerase II transcription
NCBI Summary:	The protein encoded by this gene is a member of the serine/arginine (SR)-rich family of pre-mRNA splicing factors, which constitute part of the spliceosome. Each of these factors contains an RNA recognition motif (RRM) for binding RNA and an RS domain for binding other proteins. The RS domain is rich in serine and arginine residues and facilitates interaction between different SR splicing factors. In addition to being critical for mRNA splicing, the SR proteins have also been shown to be involved in mRNA export from the nucleus and in translation. Two pseudogenes, one on chromosome 15 and the other on chromosome 21, have been found for this gene. [provided by RefSeq, Sep 2010]
UniProt Code:	Q13242
NCBI GenInfo Identifier:	3929377
NCBI Gene ID:	8683
NCBI Accession:	Q13242.1
UniProt Secondary Accession:	Q13242,Q52LD1,
UniProt Related Accession:	Q13242
Molecular Weight:	221
NCBI Full Name:	Serine/arginine-rich splicing factor 9
NCBI Synonym Full Names:	serine/arginine-rich splicing factor 9
NCBI Official Symbol:	SRSF9
NCBI Official Synonym Symbols:	SFRS9; SRp30c
NCBI Protein Information:	serine/arginine-rich splicing factor 9; SR splicing factor 9; pre-mRNA-splicing factor SRp30C; splicing factor, arginine/serine-rich 9
UniProt Protein Name:	Serine/arginine-rich splicing factor 9
UniProt Synonym Protein Names:	Pre-mRNA-splicing factor SRp30C; Splicing factor, arginine/serine-rich 9
Protein Family:	Serine/arginine-rich splicing factor
UniProt Gene Name:	SRSF9
UniProt Entry Name:	SRSF9_HUMAN

	Western blot analysis of extracts of various cell lines, using SRSF9 antibody (CAB6070) at 1:1000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (CABS014) at 1:10000 dilution. Lysates/proteins: 25ug per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Enhanced Kit (RM00021). Exposure time: 10s.
	Immunofluorescence analysis of U2OS cells using SRSF9 antibody (CAB6070) at dilution of 1:100. Blue: DAPI for nuclear staining.