Cell Biology Antibodies 1

Anti-SFPQ Antibody (CAB0958)

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SKU:
CAB0958
Product Type:
Antibody
Reactivity:
Human
Reactivity:
Mouse
Reactivity:
Rat
Host Species:
Rabbit
Isotype:
IgG
Antibody Type:
Polyclonal Antibody
Research Area:
Cell Biology

Description

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Antibody Name:Anti-SFPQ Antibody
Antibody SKU:CAB0958
Antibody Size:20uL, 50uL, 100uL
Application:WB IHC IF
Reactivity:Human, Mouse, Rat
Host Species:Rabbit
Immunogen:Recombinant fusion protein containing a sequence corresponding to amino acids 578-707 of human SFPQ (NP_005057.1).
Application:WB IHC IF
Recommended Dilution:WB 1:500 - 1:1000 IHC 1:50 - 1:100 IF 1:50 - 1:100
Reactivity:Human, Mouse, Rat
Positive Samples:OVCAR3, HeLa, Jurkat, HepG2, Mouse brain, Mouse liver, Mouse spleen, Rat brain
Immunogen:Recombinant fusion protein containing a sequence corresponding to amino acids 578-707 of human SFPQ (NP_005057.1).
Purification Method:Affinity purification
Storage Buffer:Store at -20'C. Avoid freeze / thaw cycles. Buffer: PBS with 0.02% sodium azide, 50% glycerol, pH7.3.
Isotype:IgG
Sequence:MMIR QREM EEQM RRQR EESY SRMG YMDP RERD MRMG GGGA MNMG DPYG SGGQ KFPP LGGG GGIG YEAN PGVP PATM SGSM MGSD MRTE RFGQ GGAG PVGG QGPR GMGP GTPA GYGR GREE YEGP NKKP RF
Gene ID:6421
Uniprot:P23246
Cellular Location:Cytoplasm, Nucleus matrix
Calculated MW:72kDa/76kDa
Observed MW:115kDa
Synonyms:SFPQ, POMP100, PPP1R140, PSF
Background:DNA- and RNA binding protein, involved in several nuclear processes. Essential pre-mRNA splicing factor required early in spliceosome formation and for splicing catalytic step II, probably as a heteromer with NONO. Binds to pre-mRNA in spliceosome C complex, and specifically binds to intronic polypyrimidine tracts. Involved in regulation of signal-induced alternative splicing. During splicing of PTPRC/CD45, a phosphorylated form is sequestered by THRAP3 from the pre-mRNA in resting T-cells; T-cell activation and subsequent reduced phosphorylation is proposed to lead to release from THRAP3 allowing binding to pre-mRNA splicing regulatotry elements which represses exon inclusion. Interacts with U5 snRNA, probably by binding to a purine-rich sequence located on the 3' side of U5 snRNA stem 1b. May be involved in a pre-mRNA coupled splicing and polyadenylation process as component of a snRNP-free complex with SNRPA/U1A. The SFPQ-NONO heteromer associated with MATR3 may play a role in nuclear retention of defective RNAs. SFPQ may be involved in homologous DNA pairing; in vitro, promotes the invasion of ssDNA between a duplex DNA and produces a D-loop formation. The SFPQ-NONO heteromer may be involved in DNA unwinding by modulating the function of topoisomerase I/TOP1; in vitro, stimulates dissociation of TOP1 from DNA after cleavage and enhances its jumping between separate DNA helices. The SFPQ-NONO heteromer binds DNA .
UniProt Protein Function:PSF: a proline-and glutamine-rich splicing factor. Essential pre-mRNA splicing factor required early in spliceosome formation. Seems to also bind DNA. The active complex is a heterotetramer of two 52 kDa and two 100 kDa subunits. Reported to be a PKC-alpha-binding protein in the cell nucleus. Two alternatively spliced isoforms have been described.
UniProt Protein Details:

Protein type:RNA splicing; RNA-binding; Nuclear receptor co-regulator

Chromosomal Location of Human Ortholog: 1p34.3

Cellular Component: nucleoplasm; nuclear matrix; cytoplasm; nucleus; paraspeckles; chromatin

Molecular Function:protein binding; histone deacetylase binding; nucleotide binding

Biological Process: alternative nuclear mRNA splicing, via spliceosome; transcription, DNA-dependent; RNA splicing; rhythmic process; negative regulation of circadian rhythm; negative regulation of transcription from RNA polymerase II promoter; DNA repair; mRNA processing; negative regulation of transcription, DNA-dependent; regulation of circadian rhythm; DNA recombination

UniProt Code:P23246
NCBI GenInfo Identifier:1709851
NCBI Gene ID:6421
NCBI Accession:P23246.2
UniProt Secondary Accession:P23246,P30808, Q5SZ71,
UniProt Related Accession:P23246
Molecular Weight:Calculated MW: 72kDa/76kDaObserved MW: 115kDa
NCBI Full Name:Splicing factor, proline- and glutamine-rich
NCBI Synonym Full Names:splicing factor proline/glutamine-rich
NCBI Official Symbol:SFPQ  
NCBI Official Synonym Symbols:PSF; POMP100; PPP1R140  
NCBI Protein Information:splicing factor, proline- and glutamine-rich; hPOMp100; 100 kDa DNA-pairing protein; PTB-associated splicing factor; PTB-associated-splicing factor; DNA-binding p52/p100 complex, 100 kDa subunit; protein phosphatase 1, regulatory subunit 140; polypyrimidine tract binding protein associated; polypyrimidine tract-binding protein-associated splicing factor; polypyrimidine tract-binding protein-associated-splicing factor; splicing factor proline/glutamine rich (polypyrimidine tract binding protein associated); splicing factor proline/glutamine rich (polypyrimidine tract-binding protein-associated)
UniProt Protein Name:Splicing factor, proline- and glutamine-rich
UniProt Synonym Protein Names:100 kDa DNA-pairing protein; hPOMp100; DNA-binding p52/p100 complex, 100 kDa subunit; Polypyrimidine tract-binding protein-associated-splicing factor; PSF; PTB-associated-splicing factor
Protein Family:Splicing factor
UniProt Gene Name:SFPQ  
UniProt Entry Name:SFPQ_HUMAN
Anti-SFPQ Antibody (CAB0958)Western blot analysis of extracts of various cell lines, using SFPQ antibody (CAB0958) at 1:1000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (CABS014) at 1:10000 dilution. Lysates/proteins: 25ug per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit (RM00020). Exposure time: 1s.
Anti-SFPQ Antibody (CAB0958)Immunohistochemistry of paraffin-embedded human esophageal using SFPQ Rabbit pAb (CAB0958) at dilution of 1:25 (40x lens).
Anti-SFPQ Antibody (CAB0958)Immunohistochemistry of paraffin-embedded human placenta using SFPQ Rabbit pAb (CAB0958) at dilution of 1:25 (40x lens).
Anti-SFPQ Antibody (CAB0958)Immunofluorescence analysis of HeLa cells using SFPQ antibody (CAB0958) at dilution of 1:100 (40x lens). Blue: DAPI for nuclear staining.
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