Cell Biology Antibodies 12

Anti-SFI1 Antibody (CAB8360)

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SKU:
CAB8360
Product Type:
Antibody
Applications:
WB
Reactivity:
Human
Host Species:
Rabbit
Isotype:
IgG
Research Area:
Cell Biology

Description

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Antibody Name:Anti-SFI1 Antibody
Antibody SKU:CAB8360
Antibody Size:20uL, 50uL, 100uL
Application:WB
Reactivity:Human
Host Species:Rabbit
Immunogen:Recombinant fusion protein containing a sequence corresponding to amino acids 972-1211 of human SFI1 (NP_055590.2).
Application:WB
Recommended Dilution:WB 1:500 - 1:2000
Reactivity:Human
Positive Samples:MCF7
Immunogen:Recombinant fusion protein containing a sequence corresponding to amino acids 972-1211 of human SFI1 (NP_055590.2).
Purification Method:Affinity purification
Storage Buffer:Store at -20'C. Avoid freeze / thaw cycles. Buffer: PBS with 0.02% sodium azide, 50% glycerol, pH7.3.
Isotype:IgG
Sequence:HSAR KQPR RPHF LLEP AQSQ RPQK PQEH GLGM AQPA APSL TRPF LAEA PTAL VPHS PLPG ALSS APGP KQPP TAST GPEL LLLP LSSF MPCG AAAP ARVS AQRA TPRD KPPV PSSL ASVP DPHL LLPG DFSA TRAG PGLS TAGS LDLE AELE EIQQ QLLH YQTT KQNL WSCR RQAS SLRR WLEL NREE PGPE DQEV EQQV QKEL EQVE MQIQ LLAE ELQA QRQP IGAC VARI QALR QALC
Gene ID:9814
Uniprot:A8K8P3
Cellular Location:Cytoplasm, centriole, centrosome, cytoskeleton, microtubule organizing center
Calculated MW:117-147kDa
Observed MW:148kDa
Synonyms:SFI1, PISD, PPP1R139, hSfi1p
Background:
UniProt Protein Function:SFI1: Plays a role in the dynamic structure of centrosome- associated contractile fibers via its interaction with CETN2. Belongs to the SFI1 family. 7 isoforms of the human protein are produced by alternative splicing.
UniProt Protein Details:

Chromosomal Location of Human Ortholog: 22q12.2

Cellular Component: centriole; cytosol

Molecular Function:protein binding; phosphatase binding

Biological Process: organelle organization and biogenesis; mitotic cell cycle; G2/M transition of mitotic cell cycle

UniProt Code:A8K8P3
NCBI GenInfo Identifier:119580387
NCBI Gene ID:9814
NCBI Accession:EAW59983.1
UniProt Secondary Accession:A8K8P3,O60289, Q2TAN8, A1L373, A1L387, A2A2L2, B1AKL9 B5MDB7, B7Z1V6, B7Z8G3, B7ZBE2, B7ZBE3,
UniProt Related Accession:A8K8P3
Molecular Weight:
NCBI Full Name:Sfi1 homolog, spindle assembly associated (yeast), isoform CRA_a
NCBI Synonym Full Names:Sfi1 homolog, spindle assembly associated (yeast)
NCBI Official Symbol:SFI1  
NCBI Official Synonym Symbols:PISD; hSfi1p; PPP1R139  
NCBI Protein Information:protein SFI1 homolog; homolog of yeast Sfi1; protein phosphatase 1, regulatory subunit 139
UniProt Protein Name:Protein SFI1 homolog
Protein Family:Protein
UniProt Gene Name:SFI1  
UniProt Entry Name:SFI1_HUMAN
Anti-SFI1 Polyclonal Antibody (CAB8360)Western blot analysis of extracts of MCF-7 cells, using SFI1 antibody (CAB8360) at 1:1000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (CABS014) at 1:10000 dilution. Lysates/proteins: 25ug per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit (RM00020). Exposure time: 5s.
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