Name: Anti-SF3B6 Antibody (CAB13097)
Brand: Epigenetics & Nuclear Signaling Antibodies 1
SKU: CAB13097
Availability: OutOfStock

Antibody Name:	Anti-SF3B6 Antibody
Antibody SKU:	CAB13097
Antibody Size:	20uL, 50uL, 100uL
Application:	WB
Reactivity:	Human, Mouse
Host Species:	Rabbit
Immunogen:	Recombinant fusion protein containing a sequence corresponding to amino acids 1-125 of human SF3B6 (NP_057131.1).

Application:	WB
Recommended Dilution:	WB 1:500 - 1:2000
Reactivity:	Human, Mouse
Positive Samples:	HeLa, K-562, Mouse spleen

Immunogen:	Recombinant fusion protein containing a sequence corresponding to amino acids 1-125 of human SF3B6 (NP_057131.1).
Purification Method:	Affinity purification
Storage Buffer:	Store at -20'C. Avoid freeze / thaw cycles. Buffer: PBS with 0.02% sodium azide, 50% glycerol, pH7.3.
Isotype:	IgG
Sequence:	MAMQ AAKR ANIR LPPE VNRI LYIR NLPY KITA EEMY DIFG KYGP IRQI RVGN TPET RGTA YVVY EDIF DAKN ACDH LSGF NVCN RYLV VLYY NANR AFQK MDTK KKEE QLKL LKEK YGIN TDPP K
Gene ID:	51639
Uniprot:	Q9Y3B4
Cellular Location:	Nucleus
Calculated MW:	14kDa
Observed MW:	14kDa

Synonyms:

SF3B6, CGI-110, HSPC175, Ht006, P14, SAP14, SAP14a, SF3B14, SF3B14a

Background:

This gene encodes a 14 kDa protein subunit of the splicing factor 3b complex. Splicing factor 3b associates with both the U2 and U11/U12 small nuclear ribonucleoprotein complexes (U2 snRNP) of spliceosomes. This 14 kDa protein interacts directly with subunit 1 of the splicing factor 3b complex. This 14 kDa protein also interacts directly with the adenosine that carries out the first transesterification step of splicing at the pre-mRNA branch site.

UniProt Protein Function:	SF3B6: subunit of the splicing factor SF3B. Necessary for the splicing of pre-mRNA. Directly contacts the pre-mRNA branch site adenosine for the first catalytic step of splicing. Enters the spliceosome and associates with the pre-mRNA branch site as part of the 17S U2 or, in the case of the minor spliceosome, as part of the 18S U11/U12 snRNP complex, and thus may facilitate the interaction of these snRNP with the branch sites of U2 and U12 respectively.
UniProt Protein Details:	Protein type:Spliceosome; RNA-binding; RNA splicing Chromosomal Location of Human Ortholog: 2p23 Cellular Component: nucleoplasm; snRNP U2; U12-dependent spliceosome Molecular Function:nucleotide binding Biological Process: blastocyst formation; gene expression; nuclear mRNA splicing, via spliceosome; RNA splicing
NCBI Summary:	This gene encodes a 14 kDa protein subunit of the splicing factor 3b complex. Splicing factor 3b associates with both the U2 and U11/U12 small nuclear ribonucleoprotein complexes (U2 snRNP) of spliceosomes. This 14 kDa protein interacts directly with subunit 1 of the splicing factor 3b complex. This 14 kDa protein also interacts directly with the adenosine that carries out the first transesterification step of splicing at the pre-mRNA branch site. [provided by RefSeq, Jul 2008]
UniProt Code:	Q9Y3B4
NCBI GenInfo Identifier:	7706326
NCBI Gene ID:	51639
NCBI Accession:	NP_057131.1
UniProt Secondary Accession:	Q9Y3B4,Q53TM1,
UniProt Related Accession:	Q9Y3B4
Molecular Weight:	14,585 Da
NCBI Full Name:	splicing factor 3B subunit 6
NCBI Synonym Full Names:	splicing factor 3b subunit 6
NCBI Official Symbol:	SF3B6
NCBI Official Synonym Symbols:	P14; Ht006; SAP14; SAP14a; SF3B14; CGI-110; HSPC175; SF3B14a
NCBI Protein Information:	splicing factor 3B subunit 6
UniProt Protein Name:	Splicing factor 3B subunit 6
UniProt Synonym Protein Names:	Pre-mRNA branch site protein p14; SF3b 14 kDa subunit; SF3B14a; Spliceosome-associated protein, 14-kDa; Splicing factor 3b, subunit 6, 14kDa
Protein Family:	Splicing factor
UniProt Gene Name:	SF3B6
UniProt Entry Name:	SF3B6_HUMAN

Western blot analysis of extracts of various cell lines, using SF3B6 antibody (CAB13097) at 1:3000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (CABS014) at 1:10000 dilution. Lysates/proteins: 25ug per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit (RM00020). Exposure time: 90s.

Antibodies

SF3B6 Antibody (PACO01470)

Secondary Antibody

Anti-HRP Goat Anti-Rabbit IgG (H+L) Antibody (CABS014)