Name: Anti-SF3B4 Antibody (CAB17608)
Brand: Cell Biology Antibodies 13
SKU: CAB17608
Availability: OutOfStock

Antibody Name:	Anti-SF3B4 Antibody
Antibody SKU:	CAB17608
Antibody Size:	20uL, 50uL, 100uL
Application:	WB
Reactivity:	Human, Mouse, Rat
Host Species:	Rabbit
Immunogen:	Recombinant fusion protein containing a sequence corresponding to amino acids 1-200 of human SF3B4 (NP_005841.1).

Application:	WB
Recommended Dilution:	WB 1:500 - 1:2000
Reactivity:	Human, Mouse, Rat
Positive Samples:	Rat spleen, Rat lung

Immunogen:	Recombinant fusion protein containing a sequence corresponding to amino acids 1-200 of human SF3B4 (NP_005841.1).
Purification Method:	Affinity purification
Storage Buffer:	Store at -20°C. Avoid freeze / thaw cycles. Buffer: PBS with 0.02% sodium azide, 50% glycerol, pH7.3.
Isotype:	IgG
Sequence:	MAAG PISE RNQD ATVY VGGL DEKV SEPL LWEL FLQA GPVV NTHM PKDR VTGQ HQGY GFVE FLSE EDAD YAIK IMNM IKLY GKPI RVNK ASAH NKNL DVGA NIFI GNLD PEID EKLL YDTF SAFG VILQ TPKI MRDP DTGN SKGY AFIN FASF DASD AAIE AMNG QYLC NRPI TVSY AFKK DSKG ERHG SAAE RLLA AQNP
Gene ID:	10262
Uniprot:	Q15427
Cellular Location:
Calculated MW:	44kDa
Observed MW:	44kDa

Western blot - SF3B4 Polyclonal Antibody (CAB17608)

Synonyms:

AFD1, Hsh49, SAP49, SF3b49, SF3B4

Background:

This gene encodes one of four subunits of the splicing factor 3B. The protein encoded by this gene cross-links to a region in the pre-mRNA immediately upstream of the branchpoint sequence in pre-mRNA in the prespliceosomal complex A. It also may be involved in the assembly of the B, C and E spliceosomal complexes. In addition to RNA-binding activity, this protein interacts directly and highly specifically with subunit 2 of the splicing factor 3B. This protein contains two N-terminal RNA-recognition motifs (RRMs), consistent with the observation that it binds directly to pre-mRNA. [provided by RefSeq, Jul 2008]

UniProt Protein Function:	SF3B4: subunit of the splicing factor SF3B required for 'A' complex assembly formed by the stable binding of U2 snRNP to the branchpoint sequence (BPS) in pre-mRNA. Sequence independent binding of SF3A/SF3B complex upstream of the branch site is essential, it may anchor U2 snRNP to the pre-mRNA. May also be involved in the assembly of the 'E' complex. SF3B4 has been found in complex 'B' and 'C' as well. Belongs also to the minor U12-dependent spliceosome, which is involved in the splicing of rare class of nuclear pre-mRNA intron.
UniProt Protein Details:	Protein type:RNA-binding; Spliceosome; RNA splicing Chromosomal Location of Human Ortholog: 1q21.2 Cellular Component: nucleoplasm; spliceosome; U12-dependent spliceosome Molecular Function:protein binding Biological Process: mRNA processing; nuclear mRNA splicing, via spliceosome; RNA splicing; RNA splicing, via transesterification reactions Disease: Acrofacial Dysostosis 1, Nager Type
NCBI Summary:	This gene encodes one of four subunits of the splicing factor 3B. The protein encoded by this gene cross-links to a region in the pre-mRNA immediately upstream of the branchpoint sequence in pre-mRNA in the prespliceosomal complex A. It also may be involved in the assembly of the B, C and E spliceosomal complexes. In addition to RNA-binding activity, this protein interacts directly and highly specifically with subunit 2 of the splicing factor 3B. This protein contains two N-terminal RNA-recognition motifs (RRMs), consistent with the observation that it binds directly to pre-mRNA. [provided by RefSeq, Jul 2008]
UniProt Code:	Q15427
NCBI GenInfo Identifier:	2500587
NCBI Gene ID:	10262
NCBI Accession:	Q15427.1
UniProt Secondary Accession:	Q15427,Q5SZ63,
UniProt Related Accession:	Q15427
Molecular Weight:	44,386 Da
NCBI Full Name:	Splicing factor 3B subunit 4
NCBI Synonym Full Names:	splicing factor 3b subunit 4
NCBI Official Symbol:	SF3B4
NCBI Official Synonym Symbols:	AFD1; Hsh49; SAP49; SF3b49
NCBI Protein Information:	splicing factor 3B subunit 4
UniProt Protein Name:	Splicing factor 3B subunit 4
UniProt Synonym Protein Names:	Pre-mRNA-splicing factor SF3b 49 kDa subunit; SF3b50; Spliceosome-associated protein 49; SAP 49
Protein Family:	Splicing factor
UniProt Gene Name:	SF3B4
UniProt Entry Name:	SF3B4_HUMAN