Antibody Name:	Anti-SF3B3 Antibody
Antibody SKU:	CAB6146
Antibody Size:	20uL, 50uL, 100uL
Application:	WB IHC IF
Reactivity:	Human, Mouse, Rat
Host Species:	Rabbit
Immunogen:	Recombinant fusion protein containing a sequence corresponding to amino acids 948-1217 of human SF3B3 (NP_036558.3).

Application:	WB IHC IF
Recommended Dilution:	WB 1:500 - 1:2000 IHC 1:50 - 1:200 IF 1:50 - 1:200
Reactivity:	Human, Mouse, Rat
Positive Samples:	Mouse ovary, Mouse lung

Immunogen:	Recombinant fusion protein containing a sequence corresponding to amino acids 948-1217 of human SF3B3 (NP_036558.3).
Purification Method:	Affinity purification
Storage Buffer:	Store at -20'C. Avoid freeze / thaw cycles. Buffer: PBS with 0.02% sodium azide, 50% glycerol, pH7.3.
Isotype:	IgG
Sequence:	VPAA IAPF QGRV LIGV GKLL RVYD LGKK KLLR KCEN KHIA NYIS GIQT IGHR VIVS DVQE SFIW VRYK RNEN QLII FADD TYPR WVTT ASLL DYDT VAGA DKFG NICV VRLP PNTN DEVD EDPT GNKA LWDR GLLN GASQ KAEV IMNY HVGE TVLS LQKT TLIP GGSE SLVY TTLS GGIG ILVP FTSH EDHD FFQH VEMH LRSE HPPL CGRD HLSF RSYY FPVK NVID GDLC EQFN SMEP NKQK NVSE ELDR TPPE VSKK LEDI RTRY AF
Gene ID:	23450
Uniprot:	Q15393
Cellular Location:	Nucleus
Calculated MW:	30kDa/44kDa/135kDa
Observed MW:	136kDa

Synonyms:

SF3B3, RSE1, SAP130, SF3b130, STAF130

Background:

This gene encodes subunit 3 of the splicing factor 3b protein complex. Splicing factor 3b, together with splicing factor 3a and a 12S RNA unit, forms the U2 small nuclear ribonucleoproteins complex (U2 snRNP). The splicing factor 3b/3a complex binds pre-mRNA upstream of the intron's branch site in a sequence independent manner and may anchor the U2 snRNP to the pre-mRNA. Splicing factor 3b is also a component of the minor U12-type spliceosome. Subunit 3 has also been identified as a component of the STAGA (SPT3-TAF(II)31-GCN5L acetylase) transcription coactivator-HAT (histone acetyltransferase) complex, and the TFTC (TATA-binding-protein-free TAF(II)-containing complex). These complexes may function in chromatin modification, transcription, splicing, and DNA repair.

UniProt Protein Function:	SF3B3: Subunit of the splicing factor SF3B required for 'A' complex assembly formed by the stable binding of U2 snRNP to the branchpoint sequence (BPS) in pre-mRNA. Sequence independent binding of SF3A/SF3B complex upstream of the branch site is essential, it may anchor U2 snRNP to the pre-mRNA. May also be involved in the assembly of the 'E' complex. Belongs also to the minor U12-dependent spliceosome, which is involved in the splicing of rare class of nuclear pre-mRNA intron. Belongs to the RSE1 family. 3 isoforms of the human protein are produced by alternative splicing.
UniProt Protein Details:	Protein type:Spliceosome; RNA-binding; RNA splicing Chromosomal Location of Human Ortholog: 16q22.1 Cellular Component: nucleolus; nucleoplasm; nucleus; small nuclear ribonucleoprotein complex; spliceosome; U12-dependent spliceosome Molecular Function:protein binding; RNA binding Biological Process: mRNA processing; negative regulation of protein catabolic process; nuclear mRNA splicing, via spliceosome; protein complex assembly; RNA splicing; RNA splicing, via transesterification reactions
NCBI Summary:	This gene encodes subunit 3 of the splicing factor 3b protein complex. Splicing factor 3b, together with splicing factor 3a and a 12S RNA unit, forms the U2 small nuclear ribonucleoproteins complex (U2 snRNP). The splicing factor 3b/3a complex binds pre-mRNA upstream of the intron's branch site in a sequence independent manner and may anchor the U2 snRNP to the pre-mRNA. Splicing factor 3b is also a component of the minor U12-type spliceosome. Subunit 3 has also been identified as a component of the STAGA (SPT3-TAF(II)31-GCN5L acetylase) transcription coactivator-HAT (histone acetyltransferase) complex, and the TFTC (TATA-binding-protein-free TAF(II)-containing complex). These complexes may function in chromatin modification, transcription, splicing, and DNA repair. [provided by RefSeq, Jul 2008]
UniProt Code:	Q15393
NCBI GenInfo Identifier:	116242787
NCBI Gene ID:	23450
NCBI Accession:	Q15393.4
UniProt Secondary Accession:	Q15393,Q6NTI8, Q96GC0, Q9BPY2, Q9UFX7, Q9UJ29,
UniProt Related Accession:	Q15393
Molecular Weight:	44,606 Da
NCBI Full Name:	Splicing factor 3B subunit 3
NCBI Synonym Full Names:	splicing factor 3b subunit 3
NCBI Official Symbol:	SF3B3
NCBI Official Synonym Symbols:	RSE1; SAP130; SF3b130; STAF130
NCBI Protein Information:	splicing factor 3B subunit 3
UniProt Protein Name:	Splicing factor 3B subunit 3
UniProt Synonym Protein Names:	Pre-mRNA-splicing factor SF3b 130 kDa subunit; SF3b130; STAF130; Spliceosome-associated protein 130; SAP 130
Protein Family:	Splicing factor
UniProt Gene Name:	SF3B3
UniProt Entry Name:	SF3B3_HUMAN

	Western blot analysis of extracts of various cell lines, using SF3B3 antibody (CAB6146) at 1:1000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (CABS014) at 1:10000 dilution. Lysates/proteins: 25ug per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Enhanced Kit (RM00021).
	Immunohistochemistry of paraffin-embedded rat brain using SF3B3 antibody (CAB6146) at dilution of 1:200 (40x lens).
	Immunohistochemistry of paraffin-embedded human prostate using SF3B3 antibody (CAB6146) at dilution of 1:200 (40x lens).
	Immunohistochemistry of paraffin-embedded mouse kidney using SF3B3 antibody (CAB6146) at dilution of 1:200 (40x lens).