Anti-SF3A3 Antibody (CAB20927)
- SKU:
- CAB20927
- Product Type:
- Antibody
- Antibody Type:
- Polyclonal Antibody
- Reactivity:
- Human
- Reactivity:
- Mouse
- Reactivity:
- Rat
- Host Species:
- Rabbit
- Isotype:
- IgG
Description
| Antibody Name: | Anti-SF3A3 Antibody (CAB20927) |
| Antibody SKU: | CAB20927 |
| Antibody Size: | 50µL, 100µL |
| Application: | Western blotting |
| Reactivity: | Human, Mouse |
| Host Species: | Rabbit |
| Immunogen: | Recombinant protein of Human SF3A3. |
| Application: | Western blotting |
| Recommended Dilution: | WB 1:500 - 1:2000 |
| Reactivity: | Human, Mouse |
| Positive Samples: | HeLa, A-431, NIH/3T3, K-562, Raji, Mouse liver, Mouse brain |
| Immunogen: | Recombinant protein of Human SF3A3. |
| Purification Method: | Affinity purification |
| Storage Buffer: | Store at -20°C. Avoid freeze / thaw cycles. Buffer: PBS with 0.02% sodium azide, 0.05% BSA, 50% glycerol, pH7.3. |
| Isotype: | IgG |
| Sequence: | Email for sequence |
| Cellular Location: | Nucleus speckle |
| Calculated MW: | 58kDa |
| Observed MW: | 62KDa |
| Synonyms: | SF3A3, PRP9, PRPF9, SAP61, SF3a60 |
| Background: | This gene encodes subunit 3 of the splicing factor 3a protein complex. The splicing factor 3a heterotrimer includes subunits 1, 2 and 3 and is necessary for the in vitro conversion of 15S U2 snRNP into an active 17S particle that performs pre-mRNA splicing. Subunit 3 interacts with subunit 1 through its amino-terminus while the zinc finger domain of subunit 3 plays a role in its binding to the 15S U2 snRNP. This gene has a pseudogene on chromosome 20. Alternative splicing results in multiple transcript variants. |
 | Western blot analysis of extracts of various cell lines, using SF3A3 antibody at 1:500 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution. Lysates/proteins: 25ug per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit. Exposure time: 1s. |
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