Name: Anti-SF3A2 Antibody (CAB10812)
Brand: Epigenetics & Nuclear Signaling Antibodies 1
SKU: CAB10812
Availability: OutOfStock

Antibody Name:	Anti-SF3A2 Antibody
Antibody SKU:	CAB10812
Antibody Size:	20uL, 50uL, 100uL
Application:	WB
Reactivity:	Mouse, Rat
Host Species:	Rabbit
Immunogen:	Recombinant fusion protein containing a sequence corresponding to amino acids 1-220 of human SF3A2 (NP_009096.2).

Application:	WB
Recommended Dilution:	WB 1:500 - 1:2000
Reactivity:	Mouse, Rat
Positive Samples:	Mouse testis, Rat testis

Immunogen:	Recombinant fusion protein containing a sequence corresponding to amino acids 1-220 of human SF3A2 (NP_009096.2).
Purification Method:	Affinity purification
Storage Buffer:	Store at -20'C. Avoid freeze / thaw cycles. Buffer: PBS with 0.02% sodium azide, 50% glycerol, pH7.3.
Isotype:	IgG
Sequence:	MDFQ HRPG GKTG SGGV ASSS ESNR DRRE RLRQ LALE TIDI NKDP YFMK NHLG SYEC KLCL TLHN NEGS YLAH TQGK KHQT NLAR RAAK EAKE APAQ PAPE KVKV EVKK FVKI GRPG YKVT KQRD SEMG QQSL LFQI DYPE IAEG IMPR HRFM SAYE QRIE PPDR RWQY LLMA AEPY ETIA FKVP SREI DKAE GKFW THWN RETK QFFL QFHF KMEK PPAP
Gene ID:	8175
Uniprot:	Q15428
Cellular Location:	Nucleus
Calculated MW:	49kDa
Observed MW:	66kDa

Synonyms:

SF3A2, PRP11, PRPF11, SAP62, SF3a66

Background:

This gene encodes subunit 2 of the splicing factor 3a protein complex. The splicing factor 3a heterotrimer includes subunits 1, 2 and 3 and is necessary for the in vitro conversion of 15S U2 snRNP into an active 17S particle that performs pre-mRNA splicing. Subunit 2 interacts with subunit 1 through its amino-terminus while the single zinc finger domain of subunit 2 plays a role in its binding to the 15S U2 snRNP. Subunit 2 may also function independently of its RNA splicing function as a microtubule-binding protein.

UniProt Protein Function:	SF3A2: Subunit of the splicing factor SF3A required for 'A' complex assembly formed by the stable binding of U2 snRNP to the branchpoint sequence (BPS) in pre-mRNA. Sequence independent binding of SF3A/SF3B complex upstream of the branch site is essential, it may anchor U2 snRNP to the pre-mRNA. May also be involved in the assembly of the 'E' complex. Belongs to the SF3A2 family.
UniProt Protein Details:	Protein type:RNA splicing; RNA-binding; Spliceosome Chromosomal Location of Human Ortholog: 19p13.3 Cellular Component: nucleoplasm; small nuclear ribonucleoprotein complex; snRNP U2; spliceosome Molecular Function:protein binding; RNA binding Biological Process: mRNA processing; nuclear mRNA 3'-splice site recognition; nuclear mRNA splicing, via spliceosome; RNA splicing
NCBI Summary:	This gene encodes subunit 2 of the splicing factor 3a protein complex. The splicing factor 3a heterotrimer includes subunits 1, 2 and 3 and is necessary for the in vitro conversion of 15S U2 snRNP into an active 17S particle that performs pre-mRNA splicing. Subunit 2 interacts with subunit 1 through its amino-terminus while the single zinc finger domain of subunit 2 plays a role in its binding to the 15S U2 snRNP. Subunit 2 may also function independently of its RNA splicing function as a microtubule-binding protein. [provided by RefSeq, Jul 2008]
UniProt Code:	Q15428
NCBI GenInfo Identifier:	20141793
NCBI Gene ID:	8175
NCBI Accession:	Q15428.2
UniProt Secondary Accession:	Q15428,O75245, B2RBU1, D6W605,
UniProt Related Accession:	Q15428
Molecular Weight:	49kDa
NCBI Full Name:	Splicing factor 3A subunit 2
NCBI Synonym Full Names:	splicing factor 3a subunit 2
NCBI Official Symbol:	SF3A2
NCBI Official Synonym Symbols:	PRP11; SAP62; PRPF11; SF3a66
NCBI Protein Information:	splicing factor 3A subunit 2
UniProt Protein Name:	Splicing factor 3A subunit 2
UniProt Synonym Protein Names:	SF3a66; Spliceosome-associated protein 62; SAP 62
Protein Family:	Splicing factor
UniProt Gene Name:	SF3A2

Western blot analysis of extracts of various cell lines, using SF3A2 antibody (CAB10812) at 1:1000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (CABS014) at 1:10000 dilution. Lysates/proteins: 25ug per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit (RM00020). Exposure time: 3s.

Antibodies

SF3A2 Antibody (PACO12133)

Secondary Antibody

Anti-HRP Goat Anti-Rabbit IgG (H+L) Antibody (CABS014)