Epigenetics & Nuclear Signaling Antibodies 4
Anti-SF1 Antibody (CAB6424)
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- SKU:
- CAB6424
- Product Type:
- Antibody
- Reactivity:
- Human
- Reactivity:
- Mouse
- Host Species:
- Rabbit
- Isotype:
- IgG
- Antibody Type:
- Polyclonal Antibody
- Research Area:
- Epigenetics and Nuclear Signaling
Description
| Antibody Name: | Anti-SF1 Antibody |
| Antibody SKU: | CAB6424 |
| Antibody Size: | 20uL, 50uL, 100uL |
| Application: | WB IHC |
| Reactivity: | Human, Mouse |
| Host Species: | Rabbit |
| Immunogen: | Recombinant fusion protein containing a sequence corresponding to amino acids 1-280 of human SF1 (NP_973727.1). |
| Application: | WB IHC |
| Recommended Dilution: | WB 1:500 - 1:2000 IHC 1:50 - 1:200 |
| Reactivity: | Human, Mouse |
| Positive Samples: | HepG2 |
| Immunogen: | Recombinant fusion protein containing a sequence corresponding to amino acids 1-280 of human SF1 (NP_973727.1). |
| Purification Method: | Affinity purification |
| Storage Buffer: | Store at -20'C. Avoid freeze / thaw cycles. Buffer: PBS with 0.02% sodium azide, 50% glycerol, pH7.3. |
| Isotype: | IgG |
| Sequence: | MATG ANAT PLDF PSKK RKRS RWNQ DTME QKTV IPGM PTVI PPGL TREQ ERAY IVQL QIED LTRK LRTG DLGI PPNP EDRS PSPE PIYN SEGK RLNT REFR TRKK LEEE RHNL ITEM VALN PDFK PPAD YKPP ATRV SDKV MIPQ DEYP EINF VGLL IGPR GNTL KNIE KECN AKIM IRGK GSVK EGKV GRKD GQML PGED EPLH ALVT ANTM ENVK KAVE QIRN ILKQ GIET PEDQ NDLR KMQL RELA RLNG TLRE DDNR ILRP WQSS ETRS ITNT TVCT |
| Gene ID: | 7536 |
| Uniprot: | Q15637 |
| Cellular Location: | Nucleus |
| Calculated MW: | 59kDa/61kDa/65kDa/67kDa/68kDa/71kDa |
| Observed MW: | 68kDa |
| Synonyms: | SF1, BBP, D11S636, MBBP, ZCCHC25, ZFM1, ZNF162 |
| Background: | This gene encodes a nuclear pre-mRNA splicing factor. The encoded protein specifically recognizes the intron branch point sequence at the 3' splice site, together with the large subunit of U2 auxiliary factor (U2AF), and is required for the early stages of spliceosome assembly. It also plays a role in nuclear pre-mRNA retention and transcriptional repression. The encoded protein contains an N-terminal U2AF ligand motif, a central hnRNP K homology motif and quaking 2 region which bind a key branch-site adenosine within the branch point sequence, a zinc knuckles domain, and a C-terminal proline-rich domain. Alternative splicing results in multiple transcript variants. |
| UniProt Protein Function: | SF1: a splicing factor necessary for the ATP-dependent first step of spliceosome assembly. Binds to the intron branch point sequence (BPS) 5'-UACUAAC-3' of the pre-mRNA. May act as transcription repressor. Phosphorylation of SF1 by cGMP-dependent protein kinase regulates spliceosome assembly. Binds U2AF2. Interacts with U1 snRNA. Binds EWSR1, FUS and TAF15. Six alternatively spliced isoforms have been described. |
| UniProt Protein Details: | Protein type:Transcription, coactivator/corepressor; Spliceosome; RNA splicing; RNA-binding Chromosomal Location of Human Ortholog: 11q13 Cellular Component: nucleoplasm; spliceosome; ribosome; nucleus Molecular Function:zinc ion binding; RNA binding; transcription corepressor activity Biological Process: nuclear mRNA 3'-splice site recognition; nuclear mRNA splicing, via spliceosome; Leydig cell differentiation; regulation of transcription, DNA-dependent; transcription, DNA-dependent; negative regulation of smooth muscle cell proliferation; male sex determination; spliceosome assembly; regulation of steroid biosynthetic process |
| NCBI Summary: | This gene encodes a nuclear pre-mRNA splicing factor. The encoded protein specifically recognizes the intron branch point sequence and is required for the early stages of spliceosome assembly. Alternate splicing results in multiple transcript variants. [provided by RefSeq, May 2010] |
| UniProt Code: | Q15637 |
| NCBI GenInfo Identifier: | 38258418 |
| NCBI Gene ID: | 7536 |
| NCBI Accession: | Q15637.4 |
| UniProt Secondary Accession: | Q15637,Q14818, Q14819, Q15913, Q8IY00, Q92744, Q92745 Q969H7, Q9BW01, Q9UEI0, B7Z1Q1, C9JJE2, |
| UniProt Related Accession: | Q15637 |
| Molecular Weight: | 65,371 Da |
| NCBI Full Name: | Splicing factor 1 |
| NCBI Synonym Full Names: | splicing factor 1 |
| NCBI Official Symbol: | SF1 |
| NCBI Official Synonym Symbols: | BBP; MBBP; ZFM1; ZNF162; D11S636; ZCCHC25 |
| NCBI Protein Information: | splicing factor 1; zinc finger protein 162; transcription factor ZFM1; zinc finger gene in MEN1 locus; mammalian branch point-binding protein |
| UniProt Protein Name: | Splicing factor 1 |
| UniProt Synonym Protein Names: | Mammalian branch point-binding protein; BBP; mBBP; Transcription factor ZFM1; Zinc finger gene in MEN1 locus; Zinc finger protein 162 |
| Protein Family: | Splicing factor |
| UniProt Gene Name: | SF1 |
| UniProt Entry Name: | SF01_HUMAN |
 | Western blot analysis of extracts of HepG2 cells, using SF1 antibody (CAB6424) at 1:1000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (CABS014) at 1:10000 dilution. Lysates/proteins: 25ug per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit (RM00020). Exposure time: 90s. |
 | Immunohistochemistry of paraffin-embedded mouse lung using SF1 Antibody (CAB6424) at dilution of 1:100 (40x lens). |
 | Immunohistochemistry of paraffin-embedded human gastric cancer using SF1 Antibody (CAB6424) at dilution of 1:100 (40x lens). |
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