Name: Anti-SETDB1 Antibody (CAB6145)[KO Validated]
Brand: KO Validated Antibodies 1
SKU: CAB6145
Availability: OutOfStock

Antibody Name:	Anti-SETDB1 Antibody [KO Validated]
Antibody SKU:	CAB6145
Antibody Size:	20uL, 50uL, 100uL
Application:	WB IHC IF
Reactivity:	Human, Mouse, Rat
Host Species:	Rabbit
Immunogen:	Recombinant fusion protein containing a sequence corresponding to amino acids 1-397 of human SETDB1 (NP_001230420.1).

Application:	WB IHC IF
Recommended Dilution:	WB 1:500 - 1:2000 IHC 1:50 - 1:100 IF 1:50 - 1:100
Reactivity:	Human, Mouse, Rat
Positive Samples:	293T

Immunogen:	Recombinant fusion protein containing a sequence corresponding to amino acids 1-397 of human SETDB1 (NP_001230420.1).
Purification Method:	Affinity purification
Storage Buffer:	Store at -20'C. Avoid freeze / thaw cycles. Buffer: PBS with 0.02% sodium azide, 50% glycerol, pH7.3.
Isotype:	IgG
Sequence:	MSSL PGCI GLDA ATAT VESE EIAE LQQA VVEE LGIS MEEL RHFI DEEL EKMD CVQQ RKKQ LAEL ETWV IQKE SEVA HVDQ LFDD ASRA VTNC ESLV KDFY SKLG LQYR DSSS EDES SRPT EIIE IPDE DDDV LSID SGDA GSRT PKDQ KLRE AMAA LRKS AQDV QKFM DAVN KKSS SQDL HKGT LSQM SGEL SKDG DLIV SMRI LGKK RTKT WHKG TLIA IQTV GPGK KYKV KFDN KGKS LLSG NHIA YDYH PPAD KLYV GSRV VAKY KDGN QVWL YAGI VAET PNVK NKLR FLIF FDDG YASY VTQS ELYP ICRP LKKT WEDI EDIS CRDF IEEY VTAY PNRP MVLL KSGQ LIKT EWEG TWWK SRVE EVDG SLVR ILFL VLFF STIL EAEV GGGG T
Gene ID:	9869
Uniprot:	Q15047
Cellular Location:	Chromosome, Nucleus
Calculated MW:	44kDa/143kDa
Observed MW:	180KDa

Synonyms:	SETDB1, ESET, H3-K9-HMTase4, KG1T, KMT1E, TDRD21
Background:	This gene encodes a histone methyltransferase which regulates histone methylation, gene silencing, and transcriptional repression. This gene has been identified as a target for treatment in Huntington Disease, given that gene silencing and transcription dysfunction likely play a role in the disease pathogenesis. Alternatively spliced transcript variants of this gene have been described.

UniProt Protein Function:	SETDB1: a protein lysine methyltransferase that specifically trimethylates K9 of histone H3 (H3K9me3), a specific tag for epigenetic transcriptional repression by recruiting HP1 (CBX1, CBX3 and/or CBX5) proteins to methylated histones. Unlike SUV39H H3K9 methyltransferase, which functions mainly in heterochromatin regions such as pericentric heterochromatin, SETDB1 functions mainly in euchromatic regions, playing a central role in the silencing of euchromatic genes. H3K9me3 is coordinated with DNA methylation. Interacts with a variety of proteins, including transcription factors (ERG), histone deacetylases (HDAC1/2), DNA methyltransferases (DNMT3A/B) and transcriptional co-repressors (mSin3A/B, MBD1, KAP-1, the ATFa-associated modulator mAM). Its activity is dependent on MBD1 and is heritably maintained through DNA replication by being recruited by CAF-1. Contains Tudor and methyl-CpG-binding domains, which may coordinate binding to methylated histones and methylated DNA, respectively. Is targeted to histone H3 by TIF1B, a factor recruited by KRAB zinc-finger proteins. Recruited by DNMT3A to silenced promoters in cancer cells. May play a role in the pathogenesis of Huntington's disease, since levels of SETDB1 and H3K9me3 are both increased in diseased brains. Belongs to the histone-lysine methyltransferase family. Suvar3-9 subfamily. Three isoforms of the human protein are produced by alternative splicing.
UniProt Protein Details:	Protein type:Methyltransferase; Methyltransferase, protein lysine; EC 2.1.1.43; Amino Acid Metabolism - lysine degradation Chromosomal Location of Human Ortholog: 1q21 Cellular Component: nucleoplasm; intracellular membrane-bound organelle; cytoplasm; nuclear chromatin; plasma membrane; nucleus Molecular Function:protein binding; DNA binding; zinc ion binding; histone-lysine N-methyltransferase activity Biological Process: establishment and/or maintenance of chromatin architecture; transcription, DNA-dependent; negative regulation of transcription from RNA polymerase II promoter; inner cell mass cell proliferation
NCBI Summary:	This gene encodes a histone methyltransferase which regulates histone methylation, gene silencing, and transcriptional repression. This gene has been identified as a target for treatment in Huntington Disease, given that gene silencing and transcription dysfunction likely play a role in the disease pathogenesis. Alternatively spliced transcript variants of this gene have been described.[provided by RefSeq, Jun 2011]
UniProt Code:	Q15047
NCBI GenInfo Identifier:	25091210
NCBI Gene ID:	9869
NCBI Accession:	Q15047.1
UniProt Related Accession:	Q15047
Molecular Weight:	180kDa
NCBI Full Name:	Histone-lysine N-methyltransferase SETDB1
NCBI Synonym Full Names:	SET domain bifurcated histone lysine methyltransferase 1
NCBI Official Symbol:	SETDB1
NCBI Official Synonym Symbols:	ESET; KG1T; KMT1E; TDRD21; H3-K9-HMTase4
NCBI Protein Information:	histone-lysine N-methyltransferase SETDB1
UniProt Protein Name:	Histone-lysine N-methyltransferase SETDB1
UniProt Synonym Protein Names:	ERG-associated protein with SET domain; ESET; Histone H3-K9 methyltransferase 4; H3-K9-HMTase 4; Lysine N-methyltransferase 1E; SET domain bifurcated 1
UniProt Gene Name:	SETDB1
UniProt Entry Name:	SETB1_HUMAN

	Western blot analysis of extracts of 293T cells, using SETDB1 antibody (CAB6145) at 1:1000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (CABS014) at 1:10000 dilution. Lysates/proteins: 25ug per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit (RM00020). Exposure time: 30s.
	Immunohistochemistry of paraffin-embedded rat brain using SETDB1 antibody (CAB6145) at dilution of 1:100 (40x lens).