Cell Biology Antibodies 11

Anti-SETD3 Antibody (CAB8071)

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SKU:
CAB8071
Product Type:
Antibody
Applications:
WB
Applications:
IP
Reactivity:
Human
Reactivity:
Mouse
Host Species:
Rabbit
Isotype:
IgG
Research Area:
Cell Biology

Description

system_update_altDatasheetsystem_update_altMSDS
Antibody Name:SETD3 Rabbit Polyclonal Antibody
Antibody SKU:CAB8071
Antibody Size:20uL, 50uL, 100uL
Application:WB IP
Reactivity:Human, Mouse
Host Species:Rabbit
Immunogen:A synthetic peptide corresponding to a sequence within amino acids 1-100 of human SETD3 (NP_115609.2).
Application:WB IP
Recommended Dilution:WB 1:500 - 1:2000 IP 1:50 - 1:100
Reactivity:Human, Mouse
Positive Samples:H460, DU145, MCF7, Mouse heart
Immunogen:A synthetic peptide corresponding to a sequence within amino acids 1-100 of human SETD3 (NP_115609.2).
Purification Method:Affinity purification
Storage Buffer:Store at -20'C. Avoid freeze / thaw cycles. Buffer: PBS with 0.02% sodium azide, 50% glycerol, pH7.3.
Isotype:IgG
Sequence:MGKK SRVK TQKS GTGA TATV SPKE ILNL TSEL LQKC SSPA PGPG KEWE EYVQ IRTL VEKI RKKQ KGLS VTFD GKRE DYFP DLMK WASE NGAS VEGF EMVN
Gene ID:84193
Uniprot:Q86TU7
Cellular Location:Nucleus
Calculated MW:32kDa/33kDa/67kDa
Observed MW:70kDa
Synonyms:SETD3, C14orf154
Background:
UniProt Protein Function:SETD3: Histone methyltransferase that methylates 'Lys-36' of histone H3 (H3K36me). H3 'Lys-36' methylation represents a specific tag for epigenetic transcriptional activation. Belongs to the SETD3 family. 3 isoforms of the human protein are produced by alternative splicing.
UniProt Protein Details:

Protein type:Methyltransferase, protein lysine; EC 2.1.1.43; Methyltransferase

Chromosomal Location of Human Ortholog: 14q32.2

Cellular Component: nuclear chromatin

Molecular Function:histone lysine N-methyltransferase activity (H3-K4 specific); transcription coactivator activity; histone lysine N-methyltransferase activity (H3-K36 specific)

Biological Process: transcription, DNA-dependent; peptidyl-lysine di-methylation; positive regulation of transcription, DNA-dependent; peptidyl-lysine tri-methylation; histone H3-K4 methylation; peptidyl-lysine mono-methylation; positive regulation of muscle cell differentiation; histone H3-K36 methylation; positive regulation of transcription from RNA polymerase II promoter

UniProt Code:Q86TU7
NCBI GenInfo Identifier:40068481
NCBI Gene ID:84193
NCBI Accession:NP_115609.2
UniProt Secondary Accession:Q86TU7,Q0VAQ2, Q659C0, Q86TU8, Q96GY9, Q9H5U5, A0PJU3 A5PLP0, B4DZE8,
UniProt Related Accession:Q86TU7
Molecular Weight:67kDa
NCBI Full Name:histone-lysine N-methyltransferase setd3 isoform a
NCBI Synonym Full Names:SET domain containing 3
NCBI Official Symbol:SETD3  
NCBI Official Synonym Symbols:C14orf154  
NCBI Protein Information:histone-lysine N-methyltransferase setd3
UniProt Protein Name:Histone-lysine N-methyltransferase setd3
UniProt Synonym Protein Names:SET domain-containing protein 3
Protein Family:Histone-lysine N-methyltransferase
UniProt Gene Name:SETD3  
UniProt Entry Name:SETD3_HUMAN
Anti-SETD3 Polyclonal Antibody (CAB8071)Western blot analysis of extracts of various cell lines, using SETD3 antibody (CAB8071) at 1:1000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (CABS014) at 1:10000 dilution. Lysates/proteins: 25ug per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit (RM00020). Exposure time: 30s.
Anti-SETD3 Polyclonal Antibody (CAB8071)Immunoprecipitation analysis of 200ug extracts of HeLa cells, using 3 ug SETD3 antibody (CAB8071). Western blot was performed from the immunoprecipitate using SETD3 antibody (CAB8071) at a dilition of 1:1000.
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