Antibody Name:	Anti-SERPINI1 Antibody
Antibody SKU:	CAB15703
Antibody Size:	20uL, 50uL, 100uL
Application:	WB IHC IF
Reactivity:	Mouse, Rat
Host Species:	Rabbit
Immunogen:	Recombinant fusion protein containing a sequence corresponding to amino acids 181-410 of human SERPINI1 (NP_005016.1).

Application:	WB IHC IF
Recommended Dilution:	WB 1:500 - 1:2000 IHC 1:50 - 1:100 IF 1:50 - 1:100
Reactivity:	Mouse, Rat
Positive Samples:	Mouse brain, Rat brain

Immunogen:	Recombinant fusion protein containing a sequence corresponding to amino acids 181-410 of human SERPINI1 (NP_005016.1).
Purification Method:	Affinity purification
Storage Buffer:	Store at -20'C. Avoid freeze / thaw cycles. Buffer: PBS with 0.02% sodium azide, 50% glycerol, pH7.3.
Isotype:	IgG
Sequence:	INAV YFKG NWKS QFRP ENTR TFSF TKDD ESEV QIPM MYQQ GEFY YGEF SDGS NEAG GIYQ VLEI PYEG DEIS MMLV LSRQ EVPL ATLE PLVK AQLV EEWA NSVK KQKV EVYL PRFT VEQE IDLK DVLK ALGI TEIF IKDA NLTG LSDN KEIF LSKA IHKS FLEV NEEG SEAA AVSG MIAI SRMA VLYP QVIV DHPF FFLI RNRR TGTI LFMG RVMH PETM NTSG HDFE EL
Gene ID:	5274
Uniprot:	Q99574
Cellular Location:	Secreted
Calculated MW:	46kDa
Observed MW:	46kDa

Synonyms:

SERPINI1, PI12

Background:

This gene encodes a member of the serpin superfamily of serine proteinase inhibitors. The protein is primarily secreted by axons in the brain, and preferentially reacts with and inhibits tissue-type plasminogen activator. It is thought to play a role in the regulation of axonal growth and the development of synaptic plasticity. Mutations in this gene result in familial encephalopathy with neuroserpin inclusion bodies (FENIB), which is a dominantly inherited form of familial encephalopathy and epilepsy characterized by the accumulation of mutant neuroserpin polymers. Multiple alternatively spliced variants, encoding the same protein, have been identified.

UniProt Protein Function:	SERPINI1: Serine protease inhibitor that inhibits plasminogen activators and plasmin but not thrombin. May be involved in the formation or reorganization of synaptic connections as well as for synaptic plasticity in the adult nervous system. May protect neurons from cell damage by tissue-type plasminogen activator. Defects in SERPINI1 are the cause of familial encephalopathy with neuroserpin inclusion bodies (FEN1B). FEN1B is characterized clinically as an autosomal dominantly inherited dementia, histologically by unique neuronal inclusion bodies and biochemically by polymers of neuroserpin. Belongs to the serpin family.
UniProt Protein Details:	Protein type:Secreted; Secreted, signal peptide Chromosomal Location of Human Ortholog: 3q26.1 Cellular Component: extracellular space Molecular Function:serine-type endopeptidase inhibitor activity Biological Process: regulation of cell adhesion; central nervous system development; peripheral nervous system development Disease: Encephalopathy, Familial, With Neuroserpin Inclusion Bodies
NCBI Summary:	This gene encodes a member of the serpin superfamily of serine proteinase inhibitors. The protein is primarily secreted by axons in the brain, and preferentially reacts with and inhibits tissue-type plasminogen activator. It is thought to play a role in the regulation of axonal growth and the development of synaptic plasticity. Mutations in this gene result in familial encephalopathy with neuroserpin inclusion bodies (FENIB), which is a dominantly inherited form of familial encephalopathy and epilepsy characterized by the accumulation of mutant neuroserpin polymers. Multiple alternatively spliced variants, encoding the same protein, have been identified. [provided by RefSeq, Jul 2008]
UniProt Code:	Q99574
NCBI GenInfo Identifier:	3183087
NCBI Gene ID:	5274
NCBI Accession:	Q99574.1
UniProt Secondary Accession:	Q99574,Q6AHZ4, A8K217, D3DNP1,
UniProt Related Accession:	Q99574
Molecular Weight:	46,427 Da
NCBI Full Name:	Neuroserpin
NCBI Synonym Full Names:	serpin peptidase inhibitor, clade I (neuroserpin), member 1
NCBI Official Symbol:	SERPINI1
NCBI Official Synonym Symbols:	PI12; neuroserpin
NCBI Protein Information:	neuroserpin; PI-12; serpin I1; peptidase inhibitor 12; serine (or cysteine) proteinase inhibitor, clade I (neuroserpin), member 1
UniProt Protein Name:	Neuroserpin
UniProt Synonym Protein Names:	Peptidase inhibitor 12; PI-12; Serpin I1
Protein Family:	Neuroserpin
UniProt Gene Name:	SERPINI1
UniProt Entry Name:	NEUS_HUMAN

	Western blot analysis of extracts of various cell lines, using SERPINI1 antibody (CAB15703) at 1:1000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (CABS014) at 1:10000 dilution. Lysates/proteins: 25ug per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Enhanced Kit (RM00021). Exposure time: 90s.
	Immunohistochemistry of paraffin-embedded rat brain using SERPINI1 antibody (CAB15703) at dilution of 1:200 (40x lens).