Cell Biology Antibodies 11
Anti-SERPINA4 Antibody (CAB7320)
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- SKU:
- CAB7320
- Product Type:
- Antibody
- Reactivity:
- Human
- Reactivity:
- Mouse
- Reactivity:
- Rat
- Host Species:
- Rabbit
- Isotype:
- IgG
- Antibody Type:
- Polyclonal Antibody
- Research Area:
- Cell Biology
Description
| Antibody Name: | Anti-SERPINA4 Antibody |
| Antibody SKU: | CAB7320 |
| Antibody Size: | 20uL, 50uL, 100uL |
| Application: | WB IHC IF |
| Reactivity: | Human, Mouse, Rat |
| Host Species: | Rabbit |
| Immunogen: | Recombinant fusion protein containing a sequence corresponding to amino acids 178-427 of human SERPINA4 (NP_006206.2). |
| Application: | WB IHC IF |
| Recommended Dilution: | WB 1:500 - 1:2000 IHC 1:50 - 1:200 IF 1:50 - 1:100 |
| Reactivity: | Human, Mouse, Rat |
| Positive Samples: | Mouse pancreas, Rat pancreas |
| Immunogen: | Recombinant fusion protein containing a sequence corresponding to amino acids 178-427 of human SERPINA4 (NP_006206.2). |
| Purification Method: | Affinity purification |
| Storage Buffer: | Store at -20'C. Avoid freeze / thaw cycles. Buffer: PBS with 0.02% sodium azide, 50% glycerol, pH7.3. |
| Isotype: | IgG |
| Sequence: | TIQL INDH VKKE TRGK IVDL VSEL KKDV LMVL VNYI YFKA LWEK PFIS SRTT PKDF YVDE NTTV RVPM MLQD QEHH WYLH DRYL PCSV LRMD YKGD ATVF FILP NQGK MREI EEVL TPEM LMRW NNLL RKRN FYKK LELH LPKF SISG SYVL DQIL PRLG FTDL FSKW ADLS GITK QQKL EASK SFHK ATLD VDEA GTEA AAAT SFAI KFFS AQTN RHIL RFNR PFLV VIFS TSTQ SVLF LGKV VDPT KP |
| Gene ID: | 5267 |
| Uniprot: | P29622 |
| Cellular Location: | Secreted |
| Calculated MW: | 48kDa |
| Observed MW: | 49kDa |
| Synonyms: | SERPINA4, KAL, KLST, KST, PI-4, PI4 |
| Background: |
| UniProt Protein Function: | SERPINA4: Inhibits human amidolytic and kininogenase activities of tissue kallikrein. Inhibition is achieved by formation of an equimolar, heat- and SDS-stable complex between the inhibitor and the enzyme, and generation of a small C-terminal fragment of the inhibitor due to cleavage at the reactive site by tissue kallikrein. Belongs to the serpin family. |
| UniProt Protein Details: | Protein type:Secreted; Secreted, signal peptide Chromosomal Location of Human Ortholog: 14q32.13 Cellular Component: extracellular space Molecular Function:serine-type endopeptidase inhibitor activity |
| UniProt Code: | P29622 |
| NCBI GenInfo Identifier: | 68067608 |
| NCBI Gene ID: | 5267 |
| NCBI Accession: | P29622.3 |
| UniProt Secondary Accession: | P29622,Q53XB5, Q86TR9, Q96BZ5, |
| UniProt Related Accession: | P29622 |
| Molecular Weight: | 48,542 Da |
| NCBI Full Name: | Kallistatin |
| NCBI Synonym Full Names: | serpin peptidase inhibitor, clade A (alpha-1 antiproteinase, antitrypsin), member 4 |
| NCBI Official Symbol: | SERPINA4 |
| NCBI Official Synonym Symbols: | KAL; KST; PI4; KLST; PI-4; kallistatin |
| NCBI Protein Information: | kallistatin; serpin A4; kallikrein inhibitor; peptidase inhibitor 4; protease inhibitor 4 (kallistatin); serine (or cysteine) proteinase inhibitor, clade A (alpha-1 antiproteinase, antitrypsin), member 4 |
| UniProt Protein Name: | Kallistatin |
| UniProt Synonym Protein Names: | Kallikrein inhibitor; Peptidase inhibitor 4; PI-4; Serpin A4 |
| Protein Family: | Kallistatin |
| UniProt Gene Name: | SERPINA4 |
| UniProt Entry Name: | KAIN_HUMAN |
 | Western blot analysis of extracts of various cell lines, using SERPINA4 antibody (CAB7320) at 1:500 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (CABS014) at 1:10000 dilution. Lysates/proteins: 25ug per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit (RM00020). Exposure time: 1s. |
 | Immunohistochemistry of paraffin-embedded mouse brain using SERPINA4 antibody (CAB7320) at dilution of 1:100 (40x lens). |
 | Immunofluorescence analysis of MCF7 cells using SERPINA4 antibody (CAB7320). Blue: DAPI for nuclear staining. |
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