Cell Cycle Antibodies 2
Anti-Septin-9 Antibody (CAB8657)
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- SKU:
- CAB8657
- Product Type:
- Antibody
- Applications:
- WB
- Reactivity:
- Human
- Reactivity:
- Mouse
- Reactivity:
- Rat
- Host Species:
- Rabbit
- Isotype:
- IgG
- Research Area:
- Cell Cycle
Description
| Antibody Name: | Anti-Septin-9 Antibody |
| Antibody SKU: | CAB8657 |
| Antibody Size: | 20uL, 50uL, 100uL |
| Application: | WB IF |
| Reactivity: | Human, Mouse, Rat |
| Host Species: | Rabbit |
| Immunogen: | Recombinant fusion protein containing a sequence corresponding to amino acids 1-110 of human SEPT9 (NP_001106964.1). |
| Application: | WB IF |
| Recommended Dilution: | WB 1:500 - 1:2000 IF 1:50 - 1:200 |
| Reactivity: | Human, Mouse, Rat |
| Positive Samples: | Jurkat, HeLa, 293T, U-87MG |
| Immunogen: | Recombinant fusion protein containing a sequence corresponding to amino acids 1-110 of human SEPT9 (NP_001106964.1). |
| Purification Method: | Affinity purification |
| Storage Buffer: | Store at -20'C. Avoid freeze / thaw cycles. Buffer: PBS with 0.02% sodium azide, 50% glycerol, pH7.3. |
| Isotype: | IgG |
| Sequence: | MEPP ASKV PEVP TAPA TDAA PKRV EIQM PKPA EAPT APSP AQTL ENSE PAPV SQLQ SRLE PKPQ PPVA EATP RSQE ATEA APSC VGDM ADTP RDAG LKQA PASR NEKA PV |
| Gene ID: | 10801 |
| Uniprot: | Q9UHD8 |
| Cellular Location: | Cytoplasm, cytoskeleton |
| Calculated MW: | 38kDa/41kDa/47kDa/52kDa/63kDa/64kDa/65kDa |
| Observed MW: | 75kDa |
| Synonyms: | SEPT9, AF17q25, MSF, MSF1, NAPB, PNUTL4, SINT1, SeptD1, septin-9 |
| Background: | This gene is a member of the septin family involved in cytokinesis and cell cycle control. This gene is a candidate for the ovarian tumor suppressor gene. Mutations in this gene cause hereditary neuralgic amyotrophy, also known as neuritis with brachial predilection. A chromosomal translocation involving this gene on chromosome 17 and the MLL gene on chromosome 11 results in acute myelomonocytic leukemia. Multiple alternatively spliced transcript variants encoding different isoforms have been described. |
| UniProt Protein Function: | Filament-forming cytoskeletal GTPase. May play a role in cytokinesis (Potential). May play a role in the internalization of 2 intracellular microbial pathogens, Listeria monocytogenes and Shigella flexneri. |
| NCBI Summary: | This gene is a member of the septin family involved in cytokinesis and cell cycle control. This gene is a candidate for the ovarian tumor suppressor gene. Mutations in this gene cause hereditary neuralgic amyotrophy, also known as neuritis with brachial predilection. A chromosomal translocation involving this gene on chromosome 17 and the MLL gene on chromosome 11 results in acute myelomonocytic leukemia. Multiple alternatively spliced transcript variants encoding different isoforms have been described.[provided by RefSeq, Mar 2009] |
| UniProt Code: | Q9UHD8 |
| NCBI GenInfo Identifier: | 18203688 |
| NCBI Gene ID: | 10801 |
| NCBI Accession: | AAH21192.1 |
| UniProt Secondary Accession: | Q9UHD8,Q96QF3, Q96QF4, Q96QF5, Q9HA04, Q9UG40, A8K2V3 B3KPM0, B4DTL9, B4E0N2, B4E274, B7Z654, |
| UniProt Related Accession: | Q9UHD8 |
| Molecular Weight: | 41,187 Da |
| NCBI Full Name: | Septin 9 |
| NCBI Synonym Full Names: | septin 9 |
| NCBI Official Symbol: | SEPT9 |
| NCBI Official Synonym Symbols: | MSF; MSF1; NAPB; SINT1; PNUTL4; SeptD1; AF17q25 |
| NCBI Protein Information: | septin-9 |
| UniProt Protein Name: | Septin-9 |
| UniProt Synonym Protein Names: | MLL septin-like fusion protein MSF-A; MLL septin-like fusion protein; Ovarian/Breast septin; Ov/Br septin; Septin D1 |
| UniProt Gene Name: | SEPT9 |
| UniProt Entry Name: | SEPT9_HUMAN |
 | Western blot analysis of extracts of various cell lines, using SEPT9 antibody (CAB8657) at 1:3000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (CABS014) at 1:10000 dilution. Lysates/proteins: 25ug per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit (RM00020). Exposure time: 5s. |
 | Confocal immunofluorescence analysis of U2OS cells using SEPT9 Polyclonal Antibody (CAB8657) at dilution of 1:100. Blue: DAPI for nuclear staining. |
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