Cell Biology Antibodies 5

Anti-SEPT8 Antibody (CAB15011)

(No reviews yet) Write a Review
SKU:
CAB15011
Product Type:
Antibody
Reactivity:
Human
Reactivity:
Mouse
Reactivity:
Rat
Host Species:
Rabbit
Isotype:
IgG
Antibody Type:
Polyclonal Antibody
Research Area:
Cell Biology

Description

system_update_altDatasheetsystem_update_altMSDS
Antibody Name:Anti-SEPT8 Antibody
Antibody SKU:CAB15011
Antibody Size:20uL, 50uL, 100uL
Application:WB IHC IF
Reactivity:Human, Mouse, Rat
Host Species:Rabbit
Immunogen:Recombinant fusion protein containing a sequence corresponding to amino acids 348-427 of human SEPT8 (NP_001287728.1).
Application:WB IHC IF
Recommended Dilution:WB 1:500 - 1:2000 IHC 1:50 - 1:200 IF 1:50 - 1:200
Reactivity:Human, Mouse, Rat
Positive Samples:U-251MG, HeLa, LO2, Mouse heart, Mouse kidney, Rat brain
Immunogen:Recombinant fusion protein containing a sequence corresponding to amino acids 348-427 of human SEPT8 (NP_001287728.1).
Purification Method:Affinity purification
Storage Buffer:Store at -20'C. Avoid freeze / thaw cycles. Buffer: PBS with 0.02% sodium azide, 50% glycerol, pH7.3.
Isotype:IgG
Sequence:NKVK ETEL ELKE KERE LHEK FEHL KRVH QEEK RKVE EKRR ELEE ETNA FNRR KAAV EALQ SQAL HATS QQPL RKDK DKKN
Gene ID:23176
Uniprot:Q92599
Cellular Location:Cytoplasm, cytoskeleton
Calculated MW:43kDa/49kDa/51kDa/55kDa
Observed MW:54kDa
Synonyms:SEPT8, 42980, SEP2, septin-8
Background:This gene is a member of the septin family of nucleotide binding proteins, originally described in yeast as cell division cycle regulatory proteins. Septins are highly conserved in yeast, Drosophila, and mouse, and appear to regulate cytoskeletal organization. Disruption of septin function disturbs cytokinesis and results in large multinucleate or polyploid cells. Multiple alternatively spliced transcript variants encoding different isoforms have been found for this gene.
UniProt Protein Function:SEPT8: Filament-forming cytoskeletal GTPase. May play a role in cytokinesis (Potential). May play a role in platelet secretion. Belongs to the septin family. 3 isoforms of the human protein are produced by alternative splicing.
UniProt Protein Details:

Protein type:Cytoskeletal; Hydrolase

Chromosomal Location of Human Ortholog: 5q31

Cellular Component: cytoskeleton; cytoplasm

Molecular Function:protein binding; GTP binding

Biological Process: cell cycle

NCBI Summary:This gene is a member of the septin family of nucleotide binding proteins, originally described in yeast as cell division cycle regulatory proteins. Septins are highly conserved in yeast, Drosophila, and mouse, and appear to regulate cytoskeletal organization. Disruption of septin function disturbs cytokinesis and results in large multinucleate or polyploid cells. Multiple alternatively spliced transcript variants encoding different isoforms have been found for this gene. [provided by RefSeq, Jul 2014]
UniProt Code:Q92599
NCBI GenInfo Identifier:45645200
NCBI Gene ID:23176
NCBI Accession:Q92599.4
UniProt Related Accession:Q92599
Molecular Weight:~ 55kDa
NCBI Full Name:Septin-8
NCBI Synonym Full Names:septin 8
NCBI Official Symbol:SEPTIN8  
NCBI Official Synonym Symbols:SEP2; SEPT8  
NCBI Protein Information:septin-8
UniProt Protein Name:Septin-8
UniProt Gene Name:SEPT8  
UniProt Entry Name:SEPT8_HUMAN
Anti-SEPT8 Antibody (CAB15011)Western blot analysis of extracts of various cell lines, using SEPT8 antibody (CAB15011) at 1:1000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (CABS014) at 1:10000 dilution. Lysates/proteins: 25ug per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit (RM00020). Exposure time: 10s.
Anti-SEPT8 Antibody (CAB15011)Immunohistochemistry of paraffin-embedded Human thyroid cancer using SEPT8 Rabbit pAb (CAB15011) at dilution of 1:100 (40x lens).
Anti-SEPT8 Antibody (CAB15011)Immunofluorescence analysis of C6 cells using 43716 antibody (CAB15011) at dilution of 1:100. Blue: DAPI for nuclear staining.
Anti-SEPT8 Antibody (CAB15011)Immunofluorescence analysis of L929 cells using 43716 antibody (CAB15011) at dilution of 1:100. Blue: DAPI for nuclear staining.
Anti-SEPT8 Antibody (CAB15011)Immunofluorescence analysis of U-2 OS cells using 43716 antibody (CAB15011) at dilution of 1:100. Blue: DAPI for nuclear staining.
View AllClose

0 Reviews

View AllClose

Related Products