Antibody Name:	Anti-SEPT5 Antibody
Antibody SKU:	CAB12953
Antibody Size:	20uL, 50uL, 100uL
Application:	WB IHC IF
Reactivity:	Human, Mouse, Rat
Host Species:	Rabbit
Immunogen:	Recombinant fusion protein containing a sequence corresponding to amino acids 1-369 of human SEPT5 (NP_002679.2).

Application:	WB IHC IF
Recommended Dilution:	WB 1:500 - 1:2000 IHC 1:100 - 1:200 IF 1:50 - 1:200
Reactivity:	Human, Mouse, Rat
Positive Samples:	Jurkat, NIH/3T3, Mouse brain, Mouse heart, Mouse kidney, Rat brain

Immunogen:	Recombinant fusion protein containing a sequence corresponding to amino acids 1-369 of human SEPT5 (NP_002679.2).
Purification Method:	Affinity purification
Storage Buffer:	Store at -20'C. Avoid freeze / thaw cycles. Buffer: PBS with 0.02% sodium azide, 50% glycerol, pH7.3.
Isotype:	IgG
Sequence:	MSTG LRYK SKLA TPED KQDI DKQY VGFA TLPN QVHR KSVK KGFD FTLM VAGE SGLG KSTL VHSL FLTD LYKD RKLL SAEE RISQ TVEI LKHT VDIE EKGV KLKL TIVD TPGF GDAV NNTE CWKP ITDY VDQQ FEQY FRDE SGLN RKNI QDNR VHCC LYFI SPFG HGLR PVDV GFMK ALHE KVNI VPLI AKAD CLVP SEIR KLKE RIRE EIDK FGIH VYQF PECD SDED EDFK QQDR ELKE SAPF AVIG SNTV VEAK GQRV RGRL YPWG IVEV ENQA HCDF VKLR NMLI RTHM HDLK DVTC DVHY ENYR AHCI QQMT SKLT QDSR MESP IPIL PLPT PDAE TEKL IRMK DEEL RRMQ EMLQ RMKQ QMQD Q
Gene ID:	5413
Uniprot:	Q99719
Cellular Location:	Cytoplasm, cytoskeleton
Calculated MW:	39kDa/42kDa
Observed MW:	43kDa

Synonyms:

SEPT5, CDCREL, CDCREL-1, CDCREL1, H5, HCDCREL-1, PNUTL1, septin-5

Background:

This gene is a member of the septin gene family of nucleotide binding proteins, originally described in yeast as cell division cycle regulatory proteins. Septins are highly conserved in yeast, Drosophila, and mouse and appear to regulate cytoskeletal organization. Disruption of septin function disturbs cytokinesis and results in large multinucleate or polyploid cells. This gene is mapped to 22q11, the region frequently deleted in DiGeorge and velocardiofacial syndromes. A translocation involving the MLL gene and this gene has also been reported in patients with acute myeloid leukemia. Alternative splicing results in multiple transcript variants. The presence of a non-consensus polyA signal (AACAAT) in this gene also results in read-through transcription into the downstream neighboring gene (GP1BB; platelet glycoprotein Ib), whereby larger, non-coding transcripts are produced.

UniProt Protein Function:	SEPT5: Filament-forming cytoskeletal GTPase. May play a role in cytokinesis (Potential). May play a role in platelet secretion. Belongs to the septin family. 2 isoforms of the human protein are produced by alternative splicing.
UniProt Protein Details:	Protein type:Cell cycle regulation; Hydrolase; Vesicle; Cytoskeletal Chromosomal Location of Human Ortholog: 22q11.21 Cellular Component: synaptic vesicle; cytoskeleton; plasma membrane; terminal button; cell cortex Molecular Function:GTPase activity; protein binding; GTP binding; structural molecule activity Biological Process: regulation of exocytosis; metabolic process; cytokinesis; synaptic vesicle targeting
NCBI Summary:	This gene is a member of the septin gene family of nucleotide binding proteins, originally described in yeast as cell division cycle regulatory proteins. Septins are highly conserved in yeast, Drosophila, and mouse and appear to regulate cytoskeletal organization. Disruption of septin function disturbs cytokinesis and results in large multinucleate or polyploid cells. This gene is mapped to 22q11, the region frequently deleted in DiGeorge and velocardiofacial syndromes. A translocation involving the MLL gene and this gene has also been reported in patients with acute myeloid leukemia. Alternative splicing results in multiple transcript variants. The presence of a non-consensus polyA signal (AACAAT) in this gene also results in read-through transcription into the downstream neighboring gene (GP1BB; platelet glycoprotein Ib), whereby larger, non-coding transcripts are produced. [provided by RefSeq, Dec 2010]
UniProt Code:	Q99719
NCBI GenInfo Identifier:	6685760
NCBI Gene ID:	5413
NCBI Accession:	Q99719.1
UniProt Secondary Accession:	Q99719,Q9Z2Q6, Q9JJM9,
UniProt Related Accession:	Q99719
Molecular Weight:	369
NCBI Full Name:	Septin-5
NCBI Synonym Full Names:	septin 5
NCBI Official Symbol:	SEPT5
NCBI Official Synonym Symbols:	H5; CDCREL; PNUTL1; CDCREL1; CDCREL-1; HCDCREL-1
NCBI Protein Information:	septin-5; peanut-like 1; platelet glycoprotein Ib beta chain; cell division control related protein 1
UniProt Protein Name:	Septin-5
UniProt Synonym Protein Names:	Cell division control-related protein 1; CDCrel-1; Peanut-like protein 1
UniProt Gene Name:	SEPT5
UniProt Entry Name:	SEPT5_HUMAN

	Western blot analysis of extracts of various cell lines, using SEPT5 antibody (CAB12953) at 1:3000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (CABS014) at 1:10000 dilution. Lysates/proteins: 25ug per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit (RM00020). Exposure time: 30s.
	Immunohistochemistry of paraffin-embedded rat brain using SEPT5 antibody (CAB12953) at dilution of 1:100 (40x lens).
	Immunohistochemistry of paraffin-embedded mouse brain using SEPT5 antibody (CAB12953) at dilution of 1:100 (40x lens).
	Immunofluorescence analysis of U-2 OS cells using 43713 antibody (CAB12953) at dilution of 1:100. Blue: DAPI for nuclear staining.