Name: Anti-SEPT3 Antibody (CAB16551)
Brand: Cell Cycle Antibodies 1
SKU: CAB16551
Availability: OutOfStock

Antibody Name:	Anti-SEPT3 Antibody
Antibody SKU:	CAB16551
Antibody Size:	20uL, 50uL, 100uL
Application:	WB IF
Reactivity:	Human, Mouse, Rat
Host Species:	Rabbit
Immunogen:	Recombinant fusion protein containing a sequence corresponding to amino acids 179-358 of human SEPT3 (NP_663786.2).

Application:	WB IF
Recommended Dilution:	WB 1:500 - 1:2000 IF 1:50 - 1:200
Reactivity:	Human, Mouse, Rat
Positive Samples:	Mouse brain, Rat brain

Immunogen:	Recombinant fusion protein containing a sequence corresponding to amino acids 179-358 of human SEPT3 (NP_663786.2).
Purification Method:	Affinity purification
Storage Buffer:	Store at -20'C. Avoid freeze / thaw cycles. Buffer: PBS with 0.02% sodium azide, 50% glycerol, pH7.3.
Isotype:	IgG
Sequence:	SPTG HSLR PLDL EFMK HLSK VVNI IPVI AKAD TMTL EEKS EFKQ RVRK ELEV NGIE FYPQ KEFD EDLE DKTE NDKI RQES MPFA VVGS DKEY QVNG KRVL GRKT PWGI IEVE NLNH CEFA LLRD FVIR THLQ DLKE VTHN IHYE TYRA KRLN DNGG LPPG EGLL GTVL PPVP ATPC PTAE
Gene ID:	55964
Uniprot:	Q9UH03
Cellular Location:	Cell junction, Cytoplasm, cytoskeleton, synapse
Calculated MW:	9kDa/40kDa
Observed MW:	41kDa

Synonyms:	SEPT3, SEP3, bK250D10.3, septin 3
Background:	This gene belongs to the septin family of GTPases. Members of this family are required for cytokinesis. Expression is upregulated by retinoic acid in a human teratocarcinoma cell line. The specific function of this gene has not been determined. Alternative splicing of this gene results in two transcript variants encoding different isoforms.

UniProt Protein Function:	SEPT3: belongs to the septin family of GTPases. Members of this family are required for cytokinesis. Expression is upregulated by retinoic acid in a human teratocarcinoma cell line. The specific function of this gene has not been determined. This gene encodes three transcript variants resulting in three distinct isoforms.
UniProt Protein Details:	Protein type:Cytoskeletal; Hydrolase; Cell cycle regulation Chromosomal Location of Human Ortholog: 22q13.2 Cellular Component: cell junction; cytoplasm; cytoskeleton; synapse Molecular Function:GTP binding; protein binding Biological Process: cell cycle; cell division
NCBI Summary:	This gene belongs to the septin family of GTPases. Members of this family are required for cytokinesis. Expression is upregulated by retinoic acid in a human teratocarcinoma cell line. The specific function of this gene has not been determined. Alternative splicing of this gene results in two transcript variants encoding different isoforms. [provided by RefSeq, Jul 2008]
UniProt Code:	Q9UH03
NCBI GenInfo Identifier:	147744590
NCBI Gene ID:	55964
NCBI Accession:	Q9UH03.3
UniProt Secondary Accession:	Q9UH03,Q2NKJ7, Q59GF7, Q6IBZ6, Q8N3P3, Q9HD35, B1AHR0
UniProt Related Accession:	Q9UH03
Molecular Weight:	9,061 Da
NCBI Full Name:	Neuronal-specific septin-3
NCBI Synonym Full Names:	septin 3
NCBI Official Symbol:	SEPT3
NCBI Official Synonym Symbols:	SEP3; bK250D10.3
NCBI Protein Information:	neuronal-specific septin-3
UniProt Protein Name:	Neuronal-specific septin-3
UniProt Gene Name:	SEPT3
UniProt Entry Name:	SEPT3_HUMAN

	Western blot analysis of extracts of various cell lines, using SEPT3 antibody (CAB16551) at 1:1000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (CABS014) at 1:10000 dilution. Lysates/proteins: 25ug per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit (RM00020). Exposure time: 1s.
	Immunofluorescence analysis of L929 cells using 43711 antibody (CAB16551) at dilution of 1:100. Blue: DAPI for nuclear staining.
	Immunofluorescence analysis of U-2 OS cells using 43711 antibody (CAB16551) at dilution of 1:100. Blue: DAPI for nuclear staining.