Cell Cycle Antibodies 1

Anti-SEPT12 Antibody (CAB15564)

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SKU:
CAB15564
Product Type:
Antibody
Reactivity:
Human
Reactivity:
Mouse
Reactivity:
Rat
Host Species:
Rabbit
Isotype:
IgG
Antibody Type:
Polyclonal Antibody
Research Area:
Cell Cycle

Description

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Antibody Name:Anti-SEPT12 Antibody
Antibody SKU:CAB15564
Antibody Size:20uL, 50uL, 100uL
Application:WB
Reactivity:Human, Mouse, Rat
Host Species:Rabbit
Immunogen:Recombinant fusion protein containing a sequence corresponding to amino acids 189-358 of human SEPT12 (NP_653206.2).
Application:WB
Recommended Dilution:WB 1:200 - 1:2000
Reactivity:Human, Mouse, Rat
Positive Samples:Mouse brain, Rat brain
Immunogen:Recombinant fusion protein containing a sequence corresponding to amino acids 189-358 of human SEPT12 (NP_653206.2).
Purification Method:Affinity purification
Storage Buffer:Store at -20'C. Avoid freeze / thaw cycles. Buffer: PBS with 0.02% sodium azide, 50% glycerol, pH7.3.
Isotype:IgG
Sequence:VVPV IARA DSLT MEER EAFR RRIQ QNLR THCI DVYP QMCF DEDI NDKI LNSK LRDR IPFA VVGA DQEH LVNG RCVL GRKT KWGI IEVE NMAH CEFP LLRD LLIR SHLQ DLKD ITHN IHYE NYRV IRLN ESHL LPRG PGWV NLAP ASPG QLTT PRTF KVCR GAHD DSDD EF
Gene ID:124404
Uniprot:Q8IYM1
Cellular Location:Cell projection, Cytoplasm, cilium, cytoskeleton, flagellum, spindle
Calculated MW:35kDa/40kDa
Observed MW:41kDa
Synonyms:SEPT12, SPGF10, septin-12
Background:This gene encodes a guanine-nucleotide binding protein and member of the septin family of cytoskeletal GTPases. Septins play important roles in cytokinesis, exocytosis, embryonic development, and membrane dynamics. Multiple transcript variants encoding different isoforms have been found for this gene.
UniProt Protein Function:SEPT12: Filament-forming cytoskeletal GTPase. May play a role in cytokinesis (Potential). Defects in SEPT12 are the cause of spermatogenic failure 10 (SPGF10). An infertility disorder caused by spermatogenesis defects. It results in decreased sperm motility, concentration, and multiple sperm structural defects. The most prominent feature is a defective sperm annulus, a ring structure that demarcates the midpiece and the principal piece of the sperm tail. Belongs to the septin family. 2 isoforms of the human protein are produced by alternative splicing.
UniProt Protein Details:

Protein type:Cell cycle regulation; Cytoskeletal; Hydrolase

Chromosomal Location of Human Ortholog: 16p13.3

Cellular Component: cleavage furrow; microtubule cytoskeleton; midbody; perinuclear region of cytoplasm; spindle; stress fiber

Molecular Function:GDP binding; GTP binding; phosphoinositide binding; protein binding; protein homodimerization activity

Disease: Spermatogenic Failure 10

NCBI Summary:This gene encodes a guanine-nucleotide binding protein and member of the septin family of cytoskeletal GTPases. Septins play important roles in cytokinesis, exocytosis, embryonic development, and membrane dynamics. Multiple transcript variants encoding different isoforms have been found for this gene. [provided by RefSeq, Oct 2011]
UniProt Code:Q8IYM1
NCBI GenInfo Identifier:74750767
NCBI Gene ID:124404
NCBI Accession:Q8IYM1.1
UniProt Secondary Accession:Q8IYM1,Q0P6B0, Q1PBH0, Q96LL0,
UniProt Related Accession:Q8IYM1
Molecular Weight:35,191 Da
NCBI Full Name:Septin-12
NCBI Synonym Full Names:septin 12
NCBI Official Symbol:SEPT12  
NCBI Official Synonym Symbols:SPGF10  
NCBI Protein Information:septin-12
UniProt Protein Name:Septin-12
UniProt Gene Name:SEPT12  
UniProt Entry Name:SEP12_HUMAN
Anti-SEPT12 Antibody (CAB15564)Western blot analysis of extracts of various cell lines, using SEPT12 antibody (CAB15564) at 1:1000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (CABS014) at 1:10000 dilution. Lysates/proteins: 25ug per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit (RM00020). Exposure time: 1s.
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