Name: Anti-SEPSECS Antibody (CAB7103)
Brand: Metabolism Antibodies 2
SKU: CAB7103
Availability: OutOfStock

Antibody Name:	Anti-SEPSECS Antibody
Antibody SKU:	CAB7103
Antibody Size:	20uL, 50uL, 100uL
Application:	WB IF
Reactivity:	Human, Mouse, Rat
Host Species:	Rabbit
Immunogen:	Recombinant fusion protein containing a sequence corresponding to amino acids 222-501 of human SEPSECS (NP_058651.3).

Application:	WB IF
Recommended Dilution:	WB 1:500 - 1:2000 IF 1:50 - 1:100
Reactivity:	Human, Mouse, Rat
Positive Samples:	293T, THP-1, Mouse kidney, Rat liver

Immunogen:	Recombinant fusion protein containing a sequence corresponding to amino acids 222-501 of human SEPSECS (NP_058651.3).
Purification Method:	Affinity purification
Storage Buffer:	Store at -20'C. Avoid freeze / thaw cycles. Buffer: PBS with 0.02% sodium azide, 50% glycerol, pH7.3.
Isotype:	IgG
Sequence:	STTS CFAP RVPD RLEE LAVI CANY DIPH IVNN AYGV QSSK CMHL IQQG ARVG RIDA FVQS LDKN FMVP VGGA IIAG FNDS FIQE ISKM YPGR ASAS PSLD VLIT LLSL GSNG YKKL LKER KEMF SYLS NQIK KLSE AYNE RLLH TPHN PISL AMTL KTLD EHRD KAVT QLGS MLFT RQVS GARV VPLG SMQT VSGY TFRG FMSH TNNY PCAY LNAA SAIG MKMQ DVDL FIKR LDRC LKAV RKER SKES DDNY DKTE DVDI EEMA LKLD NVLL DTYQ DASS
Gene ID:	51091
Uniprot:	Q9HD40
Cellular Location:	Cytoplasm
Calculated MW:	5kDa/55kDa
Observed MW:	56kDa

Synonyms:	SEPSECS, LP, PCH2D, SLA, SLA/LP
Background:	The amino acid selenocysteine is the only amino acid that does not have its own tRNA synthetase. Instead, this amino acid is synthesized on its cognate tRNA in a three step process. The protein encoded by this gene catalyzes the third step in the process, the conversion of O-phosphoseryl-tRNA(Sec) to selenocysteinyl-tRNA(Sec).

UniProt Protein Function:	SLA/LP: Converts O-phosphoseryl-tRNA(Sec) to selenocysteinyl- tRNA(Sec) required for selenoprotein biosynthesis. Defects in SEPSECS are the cause of pontocerebellar hypoplasia type 2D (PCH2D). PCH2D is a disorder characterized by postnatal onset of progressive atrophy of the cerebrum and cerebellum, microcephaly, profound mental retardation, spasticity, and variable seizures. Belongs to the SepSecS family. 3 isoforms of the human protein are produced by alternative splicing.
UniProt Protein Details:	Protein type:Transferase; EC 2.9.1.2; RNA-binding Chromosomal Location of Human Ortholog: 4p15.2 Cellular Component: cytoplasm; nucleus Molecular Function:protein binding; transferase activity, transferring selenium-containing groups; tRNA binding; pyridoxal phosphate binding Biological Process: selenocysteine incorporation Disease: Pontocerebellar Hypoplasia, Type 2d
NCBI Summary:	The amino acid selenocysteine is the only amino acid that does not have its own tRNA synthetase. Instead, this amino acid is synthesized on its cognate tRNA in a three step process. The protein encoded by this gene catalyzes the third step in the process, the conversion of O-phosphoseryl-tRNA(Sec) to selenocysteinyl-tRNA(Sec).[provided by RefSeq, Mar 2011]
UniProt Code:	Q9HD40
NCBI GenInfo Identifier:	62287911
NCBI Gene ID:	51091
NCBI Accession:	Q9HD40.2
UniProt Secondary Accession:	Q9HD40,Q0D2P3, Q17RT1, Q9NXZ5, Q9UGM9, Q9Y353, A8K8W1
UniProt Related Accession:	Q9HD40
Molecular Weight:	501
NCBI Full Name:	O-phosphoseryl-tRNA(Sec) selenium transferase
NCBI Synonym Full Names:	Sep (O-phosphoserine) tRNA:Sec (selenocysteine) tRNA synthase
NCBI Official Symbol:	SEPSECS
NCBI Official Synonym Symbols:	LP; SLA; PCH2D; SLA/LP
NCBI Protein Information:	O-phosphoseryl-tRNA(Sec) selenium transferase; O-phosphoseryl-tRNA(Sec) selenium transferase; SLA-p35; SLA/LP autoantigen; soluble liver antigen; liver-pancreas antigen; UGA suppressor tRNA-associated protein; tRNA(Ser/Sec)-associated antigenic protein; soluble liver antigen/liver pancreas antigen
UniProt Protein Name:	O-phosphoseryl-tRNA(Sec) selenium transferase
UniProt Synonym Protein Names:	Liver-pancreas antigen; LP; SLA-p35; SLA/LP autoantigen; Selenocysteine synthase; Sec synthase; Selenocysteinyl-tRNA(Sec) synthase; Sep-tRNA:Sec-tRNA synthase; SepSecS; Soluble liver antigen; SLA; UGA suppressor tRNA-associated protein; tRNA(Ser/Sec)-associated antigenic protein
Protein Family:	O-phosphoseryl-tRNA(Sec) selenium transferase
UniProt Gene Name:	SEPSECS
UniProt Entry Name:	SPCS_HUMAN

	Western blot analysis of extracts of various cell lines, using SEPSECS Antibody (CAB7103) at 1:1000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (CABS014) at 1:10000 dilution. Lysates/proteins: 25ug per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit (RM00020). Exposure time: 3s.
	Immunofluorescence analysis of U2OS cells using SEPSECS antibody (CAB7103). Blue: DAPI for nuclear staining.