Cell Biology Antibodies 10

Anti-SEPHS1 Antibody (CAB6454)

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SKU:
CAB6454
Product Type:
Antibody
Reactivity:
Human
Reactivity:
Rat
Host Species:
Rabbit
Isotype:
IgG
Antibody Type:
Polyclonal Antibody
Research Area:
Cell Biology

Description

system_update_altDatasheetsystem_update_altMSDS
Antibody Name:Anti-SEPHS1 Antibody
Antibody SKU:CAB6454
Antibody Size:20uL, 50uL, 100uL
Application:WB IHC IF
Reactivity:Human, Rat
Host Species:Rabbit
Immunogen:Recombinant fusion protein containing a sequence corresponding to amino acids 1-392 of human SEPHS1 (NP_036379.2).
Application:WB IHC IF
Recommended Dilution:WB 1:500 - 1:2000 IHC 1:50 - 1:200 IF 1:10 - 1:100
Reactivity:Human, Rat
Positive Samples:BT-474, SW620, HepG2, Rat kidney
Immunogen:Recombinant fusion protein containing a sequence corresponding to amino acids 1-392 of human SEPHS1 (NP_036379.2).
Purification Method:Affinity purification
Storage Buffer:Store at -20'C. Avoid freeze / thaw cycles. Buffer: PBS with 0.02% sodium azide, 50% glycerol, pH7.3.
Isotype:IgG
Sequence:MSTR ESFN PESY ELDK SFRL TRFT ELKG TGCK VPQD VLQK LLES LQEN HFQE DEQF LGAV MPRL GIGM DTCV IPLR HGGL SLVQ TTDY IYPI VDDP YMMG RIAC ANVL SDLY AMGV TECD NMLM LLGV SNKM TDRE RDKV MPLI IQGF KDAA EEAG TSVT GGQT VLNP WIVL GGVA TTVC QPNE FIMP DNAV PGDV LVLT KPLG TQVA VAVH QWLD IPEK WNKI KLVV TQED VELA YQEA MMNM ARLN RTAA GLMH TFNA HAAT DITG FGIL GHAQ NLAK QQRN EVSF VIHN LPVL AKMA AVSK ACGN MFGL MHGT CPET SGGL LICL PREQ AARF CAEI KSPK YGEG HQAW IIGI VEKG NRTA RIID KPRI IEVA PQVA TQNV NPTP GATS
Gene ID:22929
Uniprot:P49903
Cellular Location:Cell membrane, Cytoplasm, Nucleus membrane
Calculated MW:35kDa/42kDa
Observed MW:43kDa
Synonyms:SEPHS1, SELD, SPS, SPS1, selenide
Background:This gene encodes an enzyme that synthesizes selenophosphate from selenide and ATP. Selenophosphate is the selenium donor used to synthesize selenocysteine, which is co-translationally incorporated into selenoproteins at in-frame UGA codons.
UniProt Protein Function:SEPHS1: Synthesizes selenophosphate from selenide and ATP. Belongs to the selenophosphate synthase 1 family. Class II subfamily.
UniProt Protein Details:

Protein type:Other Amino Acids Metabolism - selenoamino acid; Kinase, other; EC 2.7.9.3

Chromosomal Location of Human Ortholog: 10p14

Cellular Component: nuclear membrane; cytoplasm; plasma membrane

Molecular Function:protein binding; protein homodimerization activity; GTP binding; selenide, water dikinase activity; protein heterodimerization activity; ATP binding

Biological Process: protein modification process; phosphorylation

NCBI Summary:This gene encodes an enzyme that synthesizes selenophosphate from selenide and ATP. Selenophosphate is the selenium donor used to synthesize selenocysteine, which is co-translationally incorporated into selenoproteins at in-frame UGA codons. [provided by RefSeq, Sep 2010]
UniProt Code:P49903
NCBI GenInfo Identifier:27151792
NCBI Gene ID:22929
NCBI Accession:P49903.2
UniProt Secondary Accession:P49903,Q5T5U8, Q5T5U9, Q9BVT4, B4DWK0, D3DRS9, D6PSQ9
UniProt Related Accession:P49903
Molecular Weight:392
NCBI Full Name:Selenide, water dikinase 1
NCBI Synonym Full Names:selenophosphate synthetase 1
NCBI Official Symbol:SEPHS1  
NCBI Official Synonym Symbols:SPS; SELD; SPS1  
NCBI Protein Information:selenide, water dikinase 1; selenium donor protein 1; selenophosphate synthase 1; selenophosphate synthetase 1 +E9; selenophosphate synthetase 1 +E9a; selenophosphate synthetase 1 major; selenophosphate synthetase 1 delta E2; selenophosphate synthetase 1 delta E8
UniProt Protein Name:Selenide, water dikinase 1
UniProt Synonym Protein Names:Selenium donor protein 1; Selenophosphate synthase 1
UniProt Gene Name:SEPHS1  
UniProt Entry Name:SPS1_HUMAN
Anti-SEPHS1 Antibody (CAB6454)Western blot analysis of extracts of various cell lines, using SEPHS1 antibody (CAB6454) at 1:1000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (CABS014) at 1:10000 dilution. Lysates/proteins: 25ug per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit (RM00020). Exposure time: 30s.
Anti-SEPHS1 Antibody (CAB6454)Immunohistochemistry of paraffin-embedded human breast using SEPHS1 Antibody (CAB6454) at dilution of 1:100 (40x lens).
Anti-SEPHS1 Antibody (CAB6454)Immunohistochemistry of paraffin-embedded human leiomyoma of uterus using SEPHS1 Antibody (CAB6454) at dilution of 1:100 (40x lens).
Anti-SEPHS1 Antibody (CAB6454)Immunofluorescence analysis of HeLa cells using SEPHS1 antibody (CAB6454). Blue: DAPI for nuclear staining.
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system_update_altDatasheetHuman SEPHS1 Recombinant Protein Selenophosphate synthetase 1 (SEPHS1) belongs to the selenophosphate synthase 1 family, Class II subfamily. It has four different isofor