KO Validated Antibodies 2

Anti-SEPHS1 Antibody (CAB19919)[KO Validated]

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SKU:
CAB19919
Product Type:
Antibody
Reactivity:
Human
Reactivity:
Rat
Host Species:
Rabbit
Isotype:
IgG
Research Area:
Cell Biology

Description

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Antibody Name:Anti-SEPHS1 Antibody [KO Validated]
Antibody SKU:CAB19919
Antibody Size:20uL, 50uL, 100uL
Application:WB IHC IF
Reactivity:Human, Rat
Host Species:Rabbit
Immunogen:Recombinant protein of human SEPHS1.
Application:WB IHC IF
Recommended Dilution:WB 1:500 - 1:2000 IHC 1:50 - 1:200 IF 1:10 - 1:100
Reactivity:Human, Rat
Positive Samples:293T
Immunogen:Recombinant protein of human SEPHS1.
Purification Method:Affinity purification
Storage Buffer:Store at -20°C. Avoid freeze / thaw cycles. Buffer: PBS with 0.02% sodium azide, 50% glycerol, pH7.3.
Isotype:IgG
Sequence:Email for sequence
Gene ID:22929
Uniprot:P49903
Cellular Location:Cell membrane, Cytoplasm, Nucleus membrane
Calculated MW:35kDa/42kDa
Observed MW:43KDa
Anti-SEPHS1 Antibody [KO Validated] (CAB19919)Western blot analysis of extracts from normal (control) and SEPHS1 knockout (KO) 293T cells, using SEPHS1 antibody (CAB19919) at 1:1000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (CABS014) at 1:10000 dilution. Lysates/proteins: 25ug per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit (RM00020). Exposure time: 1s.
Synonyms:SEPHS1, SELD, SPS, SPS1, selenide
Background:This gene encodes an enzyme that synthesizes selenophosphate from selenide and ATP. Selenophosphate is the selenium donor used to synthesize selenocysteine, which is co-translationally incorporated into selenoproteins at in-frame UGA codons.
UniProt Protein Function:SEPHS1: Synthesizes selenophosphate from selenide and ATP. Belongs to the selenophosphate synthase 1 family. Class II subfamily.
UniProt Protein Details:

Protein type:Other Amino Acids Metabolism - selenoamino acid; Kinase, other; EC 2.7.9.3

Chromosomal Location of Human Ortholog: 10p14

Cellular Component: nuclear membrane; cytoplasm; plasma membrane

Molecular Function:protein binding; protein homodimerization activity; GTP binding; selenide, water dikinase activity; protein heterodimerization activity; ATP binding

Biological Process: protein modification process; phosphorylation

NCBI Summary:This gene encodes an enzyme that synthesizes selenophosphate from selenide and ATP. Selenophosphate is the selenium donor used to synthesize selenocysteine, which is co-translationally incorporated into selenoproteins at in-frame UGA codons. [provided by RefSeq, Sep 2010]
UniProt Code:P49903
NCBI GenInfo Identifier:27151792
NCBI Gene ID:22929
NCBI Accession:P49903.2
UniProt Secondary Accession:P49903,Q5T5U8, Q5T5U9, Q9BVT4, B4DWK0, D3DRS9, D6PSQ9
UniProt Related Accession:P49903
Molecular Weight:392
NCBI Full Name:Selenide, water dikinase 1
NCBI Synonym Full Names:selenophosphate synthetase 1
NCBI Official Symbol:SEPHS1  
NCBI Official Synonym Symbols:SPS; SELD; SPS1  
NCBI Protein Information:selenide, water dikinase 1; selenium donor protein 1; selenophosphate synthase 1; selenophosphate synthetase 1 +E9; selenophosphate synthetase 1 +E9a; selenophosphate synthetase 1 major; selenophosphate synthetase 1 delta E2; selenophosphate synthetase 1 delta E8
UniProt Protein Name:Selenide, water dikinase 1
UniProt Synonym Protein Names:Selenium donor protein 1; Selenophosphate synthase 1
UniProt Gene Name:SEPHS1  
UniProt Entry Name:SPS1_HUMAN
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